PDF电子书：Manipulating the Mouse Embryo: A Laboratory Manual (Third Edition)

hualin840518

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' s- n0 |, @4 `悬赏求Manipulating the Mouse Embryo: A Laboratory Manual (Third Edition)！（冷泉港实验室出版）以前有两个老版本，求第三版！$ E8 y9 j3 H( ?1 J
Subject Area(s):  Biotechnology; Developmental Biology; Mouse Biology; Laboratory Manuals/Handbooks1 o& ~: P: r* e( W3 X6 ^

: u$ K0 j4 t: G1 n# jBy Andras Nagy, Samuel Lunenfeld Research Institute; Marina Gertsenstein, Samuel Lunenfeld Research Institute; Kristina Vintersten, European Molecular Biology Laboratory; Richard Behringer, University of Texas M.D. Anderson Cancer Center ' f6 L! j; b  K- n5 H
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Description
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Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting–edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields.
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$ J) h% {/ z: U, ^& [8 EKey features of the third edition:
- @8 k$ R6 r, [/ k% |7 R•New authors are leading authorities in mouse development and genetics, as were authors of prior editions.! K% d2 j" q' R- g1 N
•All new illustration and new design: All illustrations have been redrawn; extensive use of new illustrations and photographs of techniques and results; a new, contemporary design presents techniques more clearly.  j+ N; o, O# R! N, [
•New explanatory text: More background information for techniques is presented as well as more extensive troubleshooting information.3 t2 t9 Z5 V) I) @
•New, functional organization and techniques.
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Contents
: Q* Z" B" c( I7 A5 l- mChapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, and Future2 n; h3 F! @$ X$ {
Chapter 2. Summary of Mouse Development
6 k$ O3 {5 Q7 w* d6 G$ wChapter 3. Production of Transgenic and Chimeric Mice: General Issues0 w2 y) q. [5 N+ P: F, B* w
Chapter 4. Recovery and In Vitro Culture of Pre-implantation–Stage Embryos+ G% p/ C6 w2 `
Chapter 5. Isolation, Culture, and Manipulation of Post-implantation Embryos/ K! @* {$ Z/ h- b* y: ^  I0 e
Chapter 6. Surgical Procedures
. z( a$ ]' j' LChapter 7. Production of Transgenic Mice' I: p1 ]3 C6 L+ Q* \: G- _! P
Chapter 8. Isolation, Culture, and Manipulation of Blastocyst–derived Stem Cells
1 O+ S/ v% U& cChapter 9. Vector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations( a+ P1 n5 h! e. w
Chapter 10. Introduction of Foreign DNA into Embryonic Stem Cells
9 i5 q1 p7 Z) f* e0 a% NChapter 11. Production of Chimeras; [& F2 N3 ]$ M# @
Chapter 12. Detection and Analysis of Mouse Genome Alterations and Specific Sequences
9 E. q! V2 ]5 ^; wChapter 13. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning
3 {2 {( M; r# a" x& xChapter 14. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection
$ R6 w# [5 v+ V# d, K! R& i% [Chapter 15. Cryopreservation, Rederivation, and Transport of Mice. }/ _+ Q$ x8 M$ C" B- `+ S
Chapter 16. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems ( N7 |2 z# _% k. b8 x1 g. A
Chapter 17. Setting up and Equipping a Micromanipulation Lab1 B6 w( ^; z8 z; ~7 G1 p
Appendices   
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Manipulating the Mouse Embryo: A Laboratory Manual (Third Edition) [Hardcover]/ X2 z* U* v8 |' m4 Q* s# N" v
Manipulating the Mouse Embryo: A Laboratory Manual
) K+ q/ A+ R, c/ _5 W6 p; V(Second Edition) [Plastic comb binding]

renee

感觉好像在哪里看过了，汗~  

dmof

感谢党和人民的关爱~~~  

老农爱科学

文笔流畅，修辞得体，深得魏晋诸朝遗风，更将唐风宋骨发扬得入木三分，能在有生之年看见楼主的这个帖子。实在是我三生之幸啊。  

分子工程师

谢谢哦  

sky蓝

21世纪，什么最重要——我！  

桦子

不错啊! 一个字牛啊!  

lalala

看完了这么强的文章，我想说点什么，但是又不知道说什么好，想来想去只想  

甘泉

这贴？不回都不行啊  

marysyq

看贴回复是好习惯