PNAS：诱导iPS细胞为可分泌胰岛素的贝塔细胞(附原文）

细胞海洋

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5 @; w- z1 _8 Q8 K# DI型糖尿病（T1D）也被称为青少年糖尿病，它通常是由于胰腺中制造胰岛素的贝塔细胞死亡导致的。如果没有定期注射胰岛素，该病可能致命。Douglas Melton及其同事开发了一种策略，用于重新编程成年成纤维细胞，并把它们转变成诱导多能干细胞，从而利用患者自身的组织制造出贝塔细胞。9 U& v. c. f! O0 i; m- Q* f
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这些T1D干细胞与提供它们的来源的患者在遗传上相匹配，这可以有助于科学家研究该病的起源。这组作者还成功地诱导T1D干细胞成为了制造胰岛素的贝塔细胞——尽管这个过程效率不高。这组作者提出，这项研究为用T1D患者的组织培养制造胰岛素的贝塔细胞提供了概念验证。9 `9 \; F8 H1 T% D2 \4 }
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这组作者说，如果与防止患者的免疫系统排斥这些新的干细胞的技术结合起来，这些细胞可以用于移植，尽管它们的主要价值首先是作为研究糖尿病如何以及为何发生的工具。（生物谷Bioon.com）
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PNAS August 31, 2009, doi: 10.1073/pnas.0906894106% S) E$ [4 p5 N% Q! N- f
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Generation of pluripotent stem cells from patients with type 1 diabetes# e0 ^' j+ G+ |0 _1 H- e3 E
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René Maehra, Shuibing Chena, Melinda Snitowa, Thomas Ludwigb, Lisa Yagasakia, Robin Golandc, Rudolph L. Leibelc and Douglas A. Meltona,1
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% I% A2 j3 K( b# X4 X4 K9 s& {aDepartment of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138; and9 N( o* ~$ y* ]# X. K
bDepartment of Pathology and Cell Biology, and; ^4 u( Q' q- J# [) ~
cDivision of Molecular Genetics and Naomi Barrie Diabetes Center, College of Physicians and Surgeons, Columbia University, New York, NY 10032
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& w: b! t8 k! m& O9 p9 RType 1 diabetes (T1D) is the result of an autoimmune destruction of pancreatic β cells. The cellular and molecular defects that cause the disease remain unknown. Pluripotent cells generated from patients with T1D would be useful for disease modeling. We show here that induced pluripotent stem (iPS) cells can be generated from patients with T1D by reprogramming their adult fibroblasts with three transcription factors (OCT4, SOX2, KLF4). T1D-specific iPS cells, termed DiPS cells, have the hallmarks of pluripotency and can be differentiated into insulin-producing cells. These results are a step toward using DiPS cells in T1D disease modeling, as well as for cell replacement therapy.8 Z) ]- K" a; p4 t$ D! O8 G
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感谢胞友Jonathan提供原文

Jonathan

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附上全文

cdcliwei

好文章，好思路！

gaoyunhezuibang

xiexie

guanjiyu

感谢分享！