求助：Improved In Vitro Development Rates of Sheep Somatic Nuclear Transfer Embryos

jayhaoli01

求助：Improved In Vitro Development Rates of Sheep Somatic Nuclear Transfer Embryos by Using a Reverse-Order Zona-Free Cloning Method! z3 {% Y% V5 b

" j1 S' y. ^% G; @# Z" r8 g" aT.T. Peura. Cloning and Stem Cells. March 2003, 5(1): 13-24. doi:10.1089/153623003321512120. 2 F" q* A" K7 M& B; r) @3 N
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Published in Volume: 5 Issue 1: July 5, 2004* m+ B- C$ u& g! Z7 R* a) A

* m8 f1 [) F% ~/ Z( {" t( V/ rT.T. Peura
; g, ]3 R* d9 `. P; KSouth Australian Research and Development Institute, Turretfield Research Centre, Rosedale, Australia.
3 e  ]( U8 e% O3 S: u; {This paper describes a modified zona-free cloning protocol for examining the effects of short-term exposure of donor nuclei to maternal chromosomal components and associated factors. In vitro matured zona-free sheep oocytes were enucleated by micromanipulator-assisted aspiration either before or after fusion with adult fibroblast or granulosa donor cells. Subsequent kinetics of donor nuclei and maternal chromatin as well as in vitro embryo development rates were recorded. The effect of an additional activation stimulus in connection with the reverse-order cloning (fusion before enucleation) and the feasibility of manual enucleation by metal blade were also studied. As a result of the simultaneous fusion and activation, most donor nuclei remained in interphase but swelled in size. Maternal chromosomes reached anaphase II-telophase II stages within 1-2 h of activation, effectively facilitating telophase enucleation with both the micromanipulator-assisted aspiration and manual bisection. A significantly higher development rate to the blastocyst stage was achieved with the reverse-order protocol, suggesting further investigation into the possible role of oocyte nucleus-associated factors in reprogramming is warranted. Overall, the reverse-order zona-free cloning method was efficient in the production of transferable cloned sheep blastocysts and may offer yet another choice of methodology in the practical application of nuclear transfer technology. 1 x4 Q* R9 A/ h& E  B; [/ P8 o
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fengqingrusi

http://www.liebertonline.com/doi ... 1512120?cookieSet=1

fengqingrusi

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http://www.liebertonline.com/doi/pdf/10.1089/153623003321512120 % R% J8 }6 f* b% A
不知道你可不可以下载,不过我已经下载到了.你要是下载不到.我可以发给你.留下邮箱~~

fanfuzi09

贴附件吧。

jayhaoli01

3# fengqingrusi
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贴附件吧！我这里下载不了！

jayhaoli01

汗了！吉大校园网下不了！还得靠自己啊！下载下来了！贴上来！
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hualin840518

不错: p* Z6 I3 y! J8 j, B
辛苦了

franklinhg

不简单的 谢谢不简单的 谢谢不简单的 谢谢