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本帖最后由 细胞海洋 于 2010-2-9 10:31 编辑 9 A$ r2 v) R- T3 D' A' e, U2 {
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Head Neck. 2010 Jan 13. [Epub ahead of print]
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Single-marker identification of head and neck squamous cell carcinoma cancer stem cells with aldehyde dehydrogenase." N1 _; k9 d/ s' ?1 e4 B D
Clay MR, Tabor M, Owen JH, Carey TE, Bradford CR, Wolf GT, Wicha MS, Prince ME.& u a$ S! \% ?3 m9 Z
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Department of Molecular and Cell Biology, University of Wisconsin, Madison, Wisconsin.- I+ A1 A9 o+ q" u: q$ ^/ X$ j
1 U2 W6 |* L5 HBACKGROUND: In accord with the cancer stem cell (CSC) theory, only a small subset of cancer cells are capable of forming tumors. We previously reported that CD44 isolates tumorigenic cells from head and neck squamous cell cancer (HNSCC). Recent studies indicate that aldehyde dehydrogenase (ALDH) activity may represent a more specific marker of CSCs. METHODS: Six primary HNSCCs were collected. Cells with high and low ALDH activity (ALDH(high)/ALDH(low)) were isolated. ALDH(high) and ALDH(low) populations were implanted into NOD/SCID mice and monitored for tumor development. RESULTS: ALDH(high) cells represented a small percentage of the tumor cells (1% to 7.8%). ALDH(high) cells formed tumors from as few as 500 cells in 24/45 implantations, whereas only 3/37 implantations of ALDH(low) cells formed tumors. CONCLUSIONS: ALDH(high) cells comprise a subpopulation cells in HNSCCs that are tumorigenic and capable of producing tumors at very low numbers. This finding indicates that ALDH activity on its own is a highly selective marker for CSCs in HNSCC. (c) 2010 Wiley Periodicals, Inc. Head Neck, 2010.0 J/ E* F2 V- h* d* ?
+ ^6 N( Y4 q+ T" t( O1 ^PMID: 20073073 [PubMed - as supplied by publisher]
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发表于 2010-1-19 12:46
发表于 2010-1-20 08:31
