PDF电子书：Current Protocols in Stem Cell Biology 2007

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FOREWORD8 {# O4 O5 ~. v& r7 [
Stem cell biology is emerging as a field in biology with tremendous therapeutic potential.
  _6 F4 X8 k+ n& X3 |Making this potential a reality requires an international effort. The recognition" S6 B8 W* r9 ]4 f
that such a promising yet multifaceted discipline needs fostering led to the establishment$ \% P" T, Y) c. ]/ ^) ]2 L
of the International Society for Stem Cell Research (ISSCR). Central to the efforts of the$ Q2 q7 a' r4 T* t4 T! |  L; Y! V
ISSCR is the development of tools to ensure the success of stem cell researchers. What
. ~: u' A5 H# Y4 @1 bbetter way to do this than to collaborate with Current Protocols to develop this valuable+ M0 g6 M8 [$ v; X7 P
compendium of protocols in stem cell biology?
' V* N& ]0 Z/ o. u" U* QStem cell researchers have developed a number of breakthrough techniques, from the. y0 q/ x& P, e- K) M$ @0 N
derivation and manipulation of pluripotent embryonic stem cells to purification and, b* v( c6 y* Q3 A5 O8 \3 ~) K6 }. U# D
transplantation of tissue-restricted stem cells from adult organs. A number of laboratories5 g7 i3 y9 a, d9 \5 h" b
have become leaders in the field as a result of developing such techniques. The more
* k! u$ w3 D+ M/ _  Sefficient scientists are at implementing new and powerful methodologies in their own
* W1 t) z% |& t) b% \laboratories, the faster stem cell biology will advance our understanding of normal
, e8 E, _& l1 P& w3 H- p( Jdevelopment and lead to the development of therapies. Thus, the availability of quality1 A4 `0 Q; F+ Y( N4 S7 v
protocols will have amajor impact on the success of the entire field. Current Protocols has* y! H1 A* y7 M  l! {; ?  w
long been the premier volume for proven in-depth protocols regarding many aspects of
/ T/ I4 q% k! r- ^  jbiology, and this volume on stem cell biology will prove a valuable addition to researchers/ Y$ {. @' n2 e7 G5 e! x* Q
worldwide.: }& K0 n' e- g7 R/ x( e1 U
Experiments in stem cell biology must be interpreted with great caution as well as7 I& g- j9 ^) F! w' A, |4 ^3 S
openness to alternative explanations. For example, the recently discovered phenomenon7 `' [' R6 |" u+ i
of cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral
8 o" ~" o0 V* Z- [tissues were initially misinterpreted as evidence for stem cell trans-differentiation.
8 T, Q* S' ]' ^, ?. CIt is very important that this compendium of protocols highlight potential pitfalls as well
! u4 G2 t0 w; G4 pas maintain the opportunity for clarification and correction when the need arises. The* c; P6 o& W: G9 z
fact that these protocols will be provided online will help ensure that researchers always
  D5 G0 i, n; R- g7 a  J% h4 ^9 R! _! ?have the latest, most up-to-date protocols available to them.# M5 u6 C/ t: \2 i" h
The stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine6 X1 P9 e# a+ N1 H  K
push to share information and interact so that the field can move forward quickly. There: i8 v( R# z6 u
is a drive to develop not only excellent basic research skills but to bring the findings, Q2 D! B( m; i4 K- G- _# G
to clinical use so that patients can benefit. As a Hematology Attending Physician at% ]1 t  ?8 Y* f- d5 H/ \
Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia3 C, _4 |2 n7 [* t: F* j
and am drawn by the need to translate our research findings into therapies to help treat a
& f# N( j. O! s+ v$ {" rnumber of diseases. The promise is great, but we need to deliver, and I believe Current4 {1 O7 b) k; B5 Y
Protocols in Stem Cell Biology will help tremendously.
1 z2 H: [6 j  fLeonard I. Zon
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