PDF电子书：推荐-Animal Transgenesis and cloning电子书(2003版）

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内容不错，介绍也比较详细，推荐转基因动物相关的看看！" v9 x) y- x. R- b- X

( n7 l* }: j2 o- C8 Q- ULouis-Marie Houdebine
+ q1 V" i1 A) S5 P! X8 oInstitut National de la Recherche Agronomique,
1 R" S3 P1 x: u# l/ o& _; @Jouy en Josas, France
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Introduction ix
+ w) l- p' g- f: i& S0 G. NAbbreviations and Acronyms xiii4 `4 j1 J; }! \( |; v, J
1 From the gene to the transgenic animal 1( f, c' S6 E7 O& Q- H
1.1 Genome composition 1
3 c, N+ P" H: e( z% v! Q7 {* [8 A1.2 Gene structure 4
1 l; Y* x5 T) o" {! Q1.3 The number of genes in genomes 7) b  D2 f" Q4 Q, D# q: _
1.4 The major techniques of genetic engineering 13% W% G* H+ T$ u7 e( F" Z# C
1.4.1 Gene cloning 13
) ~, K5 g0 j2 m1.4.2 DNA sequencing 14* a$ r  q* d8 _5 w9 g# r
1.4.3 In vitro gene amplification 14
" q. y6 p5 C9 D) h- q( ?1.4.4 Gene construction 14
# y7 C; @) t, Y0 D* y1.4.5 Gene transfer into cells 16! G) i( d' A: O, S% z. R- L
1.5 The systematic description of genomes 21
: g' `) i$ p  A) W1.6 Classical genetic selection 269 t0 f7 j' X$ F+ U
1.7 Experimental mutation in genomes 27
0 {3 t3 I: e' k: B) z9 V6 h" F1.7.1 Chemical mutagenesis 27
+ R! N( s/ R, Y: ?2 O1.7.2 Mutagenesis by integration of foreign DNA 29
9 ?3 q! a( h& ^! X- s: W  L, v0 b1.7.3 Mutagenesis by transgenesis 30/ v* Y4 `0 T6 u9 n: D# G
2 Techniques for cloning and transgenesis 338 T  ^2 B# ^& p' v7 w# ?2 y( m
2.1 Cloning 33
0 e' ?% w) d% l* C: {5 i3 Y2.1.1 The main steps of differentiation 332 n4 S6 K! X2 g8 G6 E  C" t
2.1.2 Cloning by nuclear transfer 37
% T6 P/ F# c) f3 z2.2 Gene therapy 48
. z. p) `  i+ x! b2.2.1 The goals of gene therapy 48& j  K0 O* g9 A$ a. J: Q2 M$ \
2.2.2 The tools of gene therapy 49' g" u1 G* z& _7 w$ T) u
2.2.3 The applications of gene therapy 52
5 {' w' A5 r5 ^2.3 Techniques of animal transgenesis 545 T  K+ t7 F* ~
2.3.1 The aims and the concept of animal transgenesis 54
* E% c$ s8 [. k2.3.2 Gene transfer into gametes 60
+ y4 z( V3 g; s% h, B2.3.3 Gene transfer into embryos 654 k! z9 q. `) r& y# x: T6 E1 w" O
2.3.4 Gene transfer via cells 69
  B' H! }. z9 l( i2 _- T0 ]0 {2.3.5 Vectors for gene addition 73( D: G: s$ _1 q4 r
2.3.6 Vectors for gene replacement 85
7 L1 S3 D8 ~& E' x) ~* [2.3.7 Vectors for the rearrangement of# }% N; C( i% p1 V6 |' ]: b
targeted genes 902 u" |/ O9 ?+ `( l0 v
2.3.8 Targeted integration of foreign genes 97, J5 z8 ]& u4 L& t, Q) X) m
2.3.9 Non-classical vectors for the recombination/ r  ^/ d+ B9 _* k7 b. _
of targeted genes 105
+ L' ]5 j8 J, H2.3.10 Vectors for gene trap 106
  j( k( f  r: Y) u) ?2.3.11 Vectors for the expression of transgenes 116
' r# f2 Y7 ~9 H6 _/ T! e: v3 Applications of cloning and transgenesis 137+ b$ d" m3 W: o- _
3.1 Applications of animal cloning 137" X  M9 r% H) u+ ]" U, i
3.1.1 Basic research 137
6 E7 e8 l" n: w# {  a3.1.2 Transgenesis 142, x! Z% K3 j/ u) q* A
3.1.3 Animal reproduction 143
4 O! s% R3 O8 T1 ], n6 D  y3 X9 q1 ^3.1.4 Human reproduction 144
6 Y  `( w% g1 _" `) N3.1.5 Therapeutic cloning 144# {9 p0 I' F' t  f: w# M( I
3.1.6 Xenografting 150
1 F$ N3 T; w4 W" i3.2 Applications of animal transgenesis 153
" x: X" Q+ U, p+ ^3.2.1 Basic research 1535 y9 A; V; ^5 y$ u
3.2.2 Study of human diseases 154! H5 s: _* ]2 P# X9 B- d5 ]
3.2.3 Pharmaceutical production 159* |% S; z/ A" E3 t: }7 k6 m
3.2.4 Xenografting 162
, i# ]  Z7 h  ~7 |3.2.5 Breeding 163
' T  @/ {5 k2 h& ^4 Limits and risks of cloning, gene therapy
9 |+ c; S7 ?7 t1 v$ M9 ]0 |and transgenesis 171% m2 a& K9 y7 ?; `
4.1 Limits and risks of cloning 1734 W+ a- o: }/ a2 A1 b( Z) ]! V
4.1.1 Reproductive cloning in humans 173; I( P: {- u& R* `& S: P
4.1.2 Reproductive cloning in animals 175
- J+ b; d3 Y% E+ H4.1.3 Therapeutic cloning 176
5 N- Y3 G) J8 I: l& k9 Z4.2 Limits and risks of gene therapy 177
) C% X  Z8 Y& i4.3 Limits and risks of transgenesis 178* v. {$ w2 m" X4 B& {" t) Z
4.3.1 Technical and theoretical limits 178
6 X& v! \  L( D0 t4.3.2 Biosafety problems in confined areas 179
+ |! ]4 @$ ?) s, i7 H- W7 S" ]" H4.3.3 The intentional dissemination of transgenic6 ~- `, F& [; P* g5 M
animals into the environment 181) a1 g5 n( Y: B9 ]3 H0 _& V0 e
4.3.4 The risks for human consumers 184- @1 f+ A; ~% H' T3 d, l
4.3.5 Transgenesis and animal welfare 1853 U3 J! K) s  f9 W; w
vi CONTENTS
% S& s* S( Z5 `: x1 V0 y8 L4.3.6 Patenting of transgenic animals 187" }: V, L. S- E' x+ V) {
4.3.7 Transgenesis in humans 1888 O% B6 E0 n5 h2 }, D% [! u% g
Conclusion and Perspectives 191
5 k% k) O6 v$ S  w1 S. YReferences 199
; T# W, w# x0 Z/ sIndex 2174 S( h* s$ _5 X3 E# O# C
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