PDF电子书：推荐-Animal Transgenesis and cloning电子书(2003版）

biored

8 e+ B' v- ]' [7 B' q0 f* S
6 E( i8 p0 ?& G, x1 F: i内容不错，介绍也比较详细，推荐转基因动物相关的看看！
& G0 [5 J9 E. X+ ^+ t7 b
8 C. f! f  y) d/ lLouis-Marie Houdebine
) A5 s$ g) m- F4 h6 W; p3 VInstitut National de la Recherche Agronomique,3 d8 ]4 Y/ X$ |" l
Jouy en Josas, France
* A9 @* [1 t  k1 h. Y
4 J$ c% R4 V0 N# I* n! B4 ?$ YIntroduction ix
: |# @2 j3 o( p  ?# @" `Abbreviations and Acronyms xiii
9 p; b# Y* B/ {1 S6 H" o( a1 From the gene to the transgenic animal 1" k3 W/ a1 c6 d0 a- d
1.1 Genome composition 1
) W- }- ]# Y3 r. Q, r1.2 Gene structure 4% K% a! L& _" l. [: j0 ^  Q+ X- Z
1.3 The number of genes in genomes 7- Y. x3 }, |: @: D  s7 {
1.4 The major techniques of genetic engineering 13+ c  ^( ]0 F4 t, o
1.4.1 Gene cloning 13! c2 E5 i$ e8 N8 G; z, d& p
1.4.2 DNA sequencing 143 x( k8 k" s' ~3 }: m- H
1.4.3 In vitro gene amplification 14
3 _8 x: y; r- V+ }% }- u. }+ p1.4.4 Gene construction 148 P5 L3 J/ L( b0 D& G; v  c: R
1.4.5 Gene transfer into cells 16' Z2 O+ ?4 ~# [9 K
1.5 The systematic description of genomes 21
- e+ H$ E7 L9 _" u1.6 Classical genetic selection 26+ @* I8 }* p) _; N5 l' v- w
1.7 Experimental mutation in genomes 27; X# b6 V9 A2 n2 {1 h+ |
1.7.1 Chemical mutagenesis 27. {. W; W+ u: ]  A: W# @! e
1.7.2 Mutagenesis by integration of foreign DNA 29
# _( U- l3 c5 r- B9 k' ]1.7.3 Mutagenesis by transgenesis 30' i2 y+ x8 c4 Y+ b% i6 ~" |
2 Techniques for cloning and transgenesis 33
" u! f) L$ c  {1 }" G( R* N& K5 l2.1 Cloning 33/ r8 A3 d. \5 e" p, y9 J% i5 ^
2.1.1 The main steps of differentiation 33
" R* g* E& b5 k$ h8 ~- T( ]2.1.2 Cloning by nuclear transfer 37
2 s0 H" S  T- }2.2 Gene therapy 48
; H; z' S% V# ]! i+ K$ ~' R2.2.1 The goals of gene therapy 489 |5 `4 P; R" a" B* h# ]
2.2.2 The tools of gene therapy 49
2 J6 x( `1 K& G# u  e: Q+ f9 L; R2.2.3 The applications of gene therapy 52
: U, ~$ F( q, c' w2.3 Techniques of animal transgenesis 542 V* v& _2 i! {7 o+ ?4 I, e
2.3.1 The aims and the concept of animal transgenesis 54
) ~1 Z9 K# w$ ?- @( g1 E% Y! V. C2.3.2 Gene transfer into gametes 60
% {; v) Q# v% M! P) d2.3.3 Gene transfer into embryos 65
* d7 L! y* k* ]- C+ e2.3.4 Gene transfer via cells 69: `2 S1 ^8 M" P. x! |- j' c
2.3.5 Vectors for gene addition 73
( l9 l6 c/ S6 N0 e2.3.6 Vectors for gene replacement 85
! h1 S# o' B5 P6 K2.3.7 Vectors for the rearrangement of
4 D) W: b( ], E; ctargeted genes 904 }$ E2 n0 I$ @
2.3.8 Targeted integration of foreign genes 971 V: J# {3 }+ J" q- v. S+ b
2.3.9 Non-classical vectors for the recombination
4 w7 e/ L$ P, lof targeted genes 105
" @" Z+ y. X# ?, O/ A) K2.3.10 Vectors for gene trap 106
9 K0 o% ]7 [, h' G2.3.11 Vectors for the expression of transgenes 1160 |3 Q& P2 Z7 Z# s) N
3 Applications of cloning and transgenesis 1377 ~8 Q* ~5 ]2 C, q# s6 m6 i
3.1 Applications of animal cloning 137
' L/ e' a& ]* a7 r% e3.1.1 Basic research 137
8 \" x  F# o, ?3.1.2 Transgenesis 142% H9 _; O5 s9 b  e  y
3.1.3 Animal reproduction 143
' w- ]! w( p. D& E5 M5 o% C3.1.4 Human reproduction 144! t* R9 [' }3 W! i: O/ n
3.1.5 Therapeutic cloning 144
; p. `4 ]1 X' ^* o" b. O  r3.1.6 Xenografting 150
7 i8 H, h! J0 ~: I' h2 f3.2 Applications of animal transgenesis 153
0 A$ Y" M) g/ E, G5 \3.2.1 Basic research 153
1 \& h  e; [0 Q5 X  J3.2.2 Study of human diseases 154
* m; M5 d' m1 ^5 c9 b9 X' v3.2.3 Pharmaceutical production 159" y8 r- W0 z/ Z4 \# {8 {2 c
3.2.4 Xenografting 1627 k( L! @5 C3 H! _1 \
3.2.5 Breeding 1630 H: i  Z" l- X
4 Limits and risks of cloning, gene therapy
6 R* k1 d* c" U* l7 _and transgenesis 171
8 B8 M6 P* D3 ^4.1 Limits and risks of cloning 173
  j! }: T. b$ \: H% k# ?4.1.1 Reproductive cloning in humans 173
5 U4 M9 f0 B" Y8 x* J! }4.1.2 Reproductive cloning in animals 175
" K$ B4 L5 c3 f# z6 Z4.1.3 Therapeutic cloning 1760 v( d6 y7 }( F8 L6 C
4.2 Limits and risks of gene therapy 1777 g: X* ^3 N( e2 U% E
4.3 Limits and risks of transgenesis 178
. m  d( d7 G$ p4.3.1 Technical and theoretical limits 178
. n1 `: w6 ~! `* u4 ^4.3.2 Biosafety problems in confined areas 179' X7 _( r  `2 Y+ p1 \+ g
4.3.3 The intentional dissemination of transgenic
* R$ k" b6 {1 H+ h. N. Nanimals into the environment 181
6 j' J& M8 C) h. e; n3 F4.3.4 The risks for human consumers 1845 v9 O+ u. }& t- }
4.3.5 Transgenesis and animal welfare 185
4 U$ O0 c  k/ M, `1 Uvi CONTENTS1 }4 G$ \) ^" t" i
4.3.6 Patenting of transgenic animals 187
7 r: f7 h& s  g4.3.7 Transgenesis in humans 188
& s: M3 c% k( [& O( L* T: d2 d/ jConclusion and Perspectives 1911 ]9 \' |8 J( E$ k. m2 @
References 199
/ U- M0 j  ?% p. v+ h' {Index 217, p: z% n3 I  f, Z' `

4 \9 o2 E$ t! H[hide][/hide]

xiaomao_zhoubo

谢谢

yibo10679

xiexie

woosheng

谢谢

baixueyao029

kankan

baixueyao029

能不能取消收费，每次遇到收费，扣老多包子不说，还下不下来，郁闷，不知道哪出错了！

wgydys

thanks

lifeida

谢谢

gzclover

非常赞的书籍，谢谢啦！

hyde

SEESEE# _  a5 z% s, ~9 Z+ A4 s( [1 h