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friendly0722

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葵花

沙发，先顶一下

liuyiyi

Tillmann S M.Isolation of ES-like cell lines from bovine and caprine preimplantation embryos. J Anim Breed Genet, 1996,

zhaichao_1

pradling A, Drummond-Barbosa D, Kai T. Stem cells and their niche. Nature. 2001;414(6859):98–104.* T: `0 Z/ i9 C, f
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Morrison SJ, Kimble J. Asymmetric and symmetric stem-cell divisions in development and cancer. Nature. 2006;441(7097):1068–74." [. a9 D2 n. {$ h- V( @0 ~* O# e

$ W5 Z/ |8 i: |6 C& d0 j  Y9 @7 p- qKai T, Spradling A. Differentiating germ cells can revert into functional stem cells in Drosophila melanogaster ovaries. Nature.2004;428(6982):564–9
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Nystul T, Spradling A. An Epithelial Nihce in the Drosophila ovary undergoes long-range stem cell replacement. Cell Stem Cell   2007;1(3):277–85.$ F) P7 ~% ~# x- ~1 L+ t, }

5 P( {  m5 v: A6 \White J, Dalton S. Cell cycle control of embryonic stem cells. Stem Cell Rev. 2
3 s/ L# R9 S+ G5 R. ~3 x2003;17:126–40.
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Niwa H. How is pluripotency determined and maintained? Develepment 2007;134:635–46.
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Boiani M, Scholer HR. Regulatory networks in embryo-& P- B4 a  B/ p1 ]9 k: P5 U0 i1 u
derived pluripotent stem cells. Nat Rev Mol Cell Biol. 2005;6:872–84.   $ j0 p$ n* H4 K/ U

+ v$ O. [2 w& ZOhtsuka S, Dalton S. Molecular and biologic properties of pluripotent embryonic stem cells. Gene Therapy. 2008;15:74–81.6 U- K, y5 W5 E% m5 y; g
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谢谢各位了！我的邮箱 zhaichao_1@126.com

hualin840518

2. Masaki H, T Ishikawa, S Takahashi et al. (2008). Heterogeneity of Pluripotent marker gene expression in Colonies generated in human iPS cell induction culture. Stem Cell Res 2:105–115.7 `5 \8 q  z, ]+ f2 I! J) L) o+ ?  a9 ^
3. Li XL, Y Eishi, YQ Bai, H Sakai, Y Akiyama, M Tani, T Takizawa, M Koike and Y Yuasa. (2004). Expression of the SRY-related HMG box protein SOX2 in human gastric carcinoma. Int J Oncol 24:257–263.( s/ X1 F0 _, ]: E* W
4. Sanada Y, K Yoshida, M Ohara, M Oeda, K Konishi and Y Tsutani. (2006). Histopathologic evaluation of stepwise progression of pancreatic carcinoma with immunohistochemical analysis of gastric epithelial transcription factor SOX2 comparison of expression patterns between invasive components and cancerous or nonneoplastic intraductal components. Pancreas 32:164–170.
* h+ t( N) b6 Y0 @% m5. Phi JH, SH Park, SK Kim, SH Paek, JH Kim, YJ LEe, BK Cho, C-K Park, D-H Lee and K-C Wang. (2008).( d7 N5 {2 e7 a
Sox2 expression in brain tumors a reflection of the neuroglial differentiation pathway. Am J Surg Pathol 32:103–112.
. ^- W" C# }+ Y1 v0 T% R6. Rowland BD and DS Peeper. (2006).
3 |7 r1 `9 y% l; m- ?5 pKLF4, p21 and context-dependent opposing forces in cancer.
! |/ y# u7 u! ~' I* xNature Rev Cancer6:11–231 n' l$ h0 _7 y) h3 Y8 Z

$ Z3 w2 ]# s+ V, w# G" C非常感谢！

labby

Asymmetric and symmetric stem-cell divisions in development and cancer

labby

Differentiating germ cells can revert into functional stem cells in Drosophila melanogaster ovaries( n1 O6 F4 V7 X; }

8 T; T% a0 Z8 }% Z4 I/ k+ F现只找到这篇了

zhbshi998

胚胎干细胞微环境及其对肿瘤的影响5 }5 A# s: n/ w6 q. F) j
Embryonic stem cell microenvironment's effects on tumors
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8 j- x+ p5 z4 Q7 f作 者： 陶丽 张伟伟 陈磊- P1 {1 _5 \2 G" C+ p
国际肿瘤学杂志，2010年

懵懂干细胞

回复 8# zhbshi998 ) {: w) l* v5 G% G8 l6 I

myrudy

1. Morphology of iPSCs during derivation: Oct4-GFP, AKP, karyotype.
/ v$ U) }8 C, l6 }$ c2. RT-PCR of known pluripotency genes, qPCR of endogenous and exogenous genes expression, staining of key factors and surface markers.
% y, Q7 Z  I2 M% E3. Southern blot, Bisulfite sequencing.
- e4 Q1 f2 Q  ]) |8 f; X5 l4.In vitro differentiation and teratoma formation.; ~1 E4 \# L! m- f8 N
5. Chimeric mice and tetraploid complemented mice generation.2 G5 h0 v! q/ X* ]& g8 L+ h
6. Genotyping of the iPS-mice.; ~3 H( d: S  u, y* Y( F. Z: `
7.Microarray analysis of gene expression.+ g. v. v* O5 G1 K1 \( t' V
麻烦帮帮忙，很急！万分感谢！！！