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本帖最后由 sunyucheng 于 2010-5-12 21:56 编辑 * O# Q+ k- b' N! O$ u# b+ M
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Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4.
' z. o$ ~0 x7 v5 ]- j+ x G. Grant Welstead, Tobias Brambrink, Rudolf Jaenisch
* ]8 x6 J$ x" U6 `! _Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology.
0 w3 e0 l' X5 o; [内容:0:10 Cellular Reprogramming10: Q5 ~9 @; h n; n) R
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3:30 Introduction210) U2 r2 O( h7 S. Y, e' y! a5 m" c; y
" ?+ E& Q) L/ D! I' Z4:32 Transfecting HEK293T Cells and Purifying Lentivirus272
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2 h1 f0 {9 _5 o! x% O, o8 Q9 N+ f/ ~6:08 Infection of MEFs with Lentivirus368: S3 Y$ D0 J/ n( e9 T- c0 t) N3 l
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8:56 Picking up the iPS Colonies536
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# p5 l+ m5 K% W) o+ G3 K" H( H11:15 Expansion of iPS Cells675
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* V# m3 h8 }1 @5 g' [; Z12:32 Conclusion752
% l, ^1 J& K0 E7 F6 X0 }& b1 w' F; Fhttp://www.jove.com/index/Details.stp?ID=734 |
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