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本帖最后由 细胞海洋 于 2011-8-18 16:35 编辑
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季旭东1 , 高 超2 , 杨月景3 , 刘计荣4: P* o+ G/ B& B. X3 k1 |9 e2 m
(11 郑州大学口腔医学院口腔内科 河南郑州 450052 ;21 郑州市儿童医院神经内科 河南郑州 450053 ;
. j. w% r7 U* Q0 y- W' \, F% I31 郑州大学第二附属医院消化内科 河南郑州 450003 ;41 郑州大学基础医学院 河南郑州 450052); Y# r" P8 t5 |7 N6 r+ y
摘要: 目的: 体外诱导人脐血单个核细胞(mononuclear cells ,MNCs) 中的神经干细胞(neural stem cells ,NSCs) 向. r9 f7 }* b/ z, O' G, X5 N+ Q C. F
神经细胞分化的研究。方法: 体外培养中用表皮生长因子(epithelium growth factor ,EGF) 和碱性成纤维细胞2 _* P; A# N. S' q6 H1 j
生长因子(basic fibroblast growth factor ,bFGF) 诱导人脐血NSCs 向神经细胞的分化。结果: 在EGF 和bFGF 作
5 C+ Q. v5 ?1 U% g. m$ o5 _用下,实验组Nestin 阳性细胞数量显著增多;在EGF 和bFGF 作用下,Nestin 的mRNA 表达PCR 条带变量两组6 G4 ?1 V" W( F& s9 W0 \
间有显著性差异。结论: 细胞因子EGF 和bFGF 在体外能促进脐血MNCs 中NSCs 的分化,联合应用较单独
2 ?3 X2 E7 E! Q b9 P应用具有更强的促进脐血MNCs 向神经样细胞分化作用。; K* t; w7 \$ o
关键词: 脐血; 神经干细胞; EGF; bFGF ^" b3 F9 B' Z4 o, u1 ?* h
中图分类号: R32912 文献标识码: A
! V4 A) x- Z* D/ d0 ~& y3 XInvestigation of differentiation derived from human umbilical$ ? u2 \) l9 X M; V
cord blood into neuron-like cells0 {, u, ]' E& x, _* c I
J I Xu-dong1 , GAO Chao2 , YANG Yue-jing3 , LIU Ji- rong48 n0 ]% u. c8 m' x+ v
( 1. Department of Oral Medicine , The College of Stomatology of Zhengzhou University , Zhengzhou 450052 , China ; 2. Department of Nerve
4 [, a9 I- |$ O* m B0 RMedicine Chidren’s Hospital of Zhengzhou , Zhengzhou 450053 , China ; 3. Department of Digestion , The Secend Affiliated Hospital of
/ O; Z1 s' H0 f/ q1 M4 dZhengzhou University , Zhengzhou 450052 , China ; 4. Department of Pathophysiology , Basic Medical School of Zhengzhou University ,) }, ?* B6 e' N, ^( v
Zhengzhou 450052 , China)
3 I0 r6 d; @5 T% v& y4 [& vAbstract : Objective : To investigate differentiation of nerve stem cells in mononuclear cells derived1 z6 ^4 i5 G7 L0 i2 i* u
from human umbilical cord blood into neuron-like cells in vitro. Methods : To induce the differentiation
8 c+ D8 Z! r2 c! rof nerve stem cells into neuron-like cells with epithelium growth factor ( EGF) and basic fibroblast j- M, l) v( R( O1 L) n: _: y
growth factor (bFGF) . Results : In the role of EGF and bFGF ,the nember of nestin’s positive cell is
- A$ I& u0 c7 Q; ?2 {significant increasing in the experimental group. The belt of PCR which is expressed by nestin’s mRNA
9 D% x# }* B" mis more brighter in experimental group ,there are significant difference in two groups. Conclusion : EGF
. D" p Y5 w4 w. Y! d: F7 Cassociated with bFGF can improve the differentiation of nerve stem cells into neuron-like cells.8 u0 T0 i- _! t5 ^1 f6 [
Keywords : mononuclear ; NSCs ; EGF ; bFGF
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发表于 2009-3-2 11:36
