MEF 到底是一种什么性质的细胞？是单一的细胞？肌纤维细胞，神经纤维细胞？

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MEF里不会是一种纯细胞，但随着代数的增加会变纯2 F# Z' s# C/ d6 f$ d; ~
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最近的一篇文章有没有看过（附件中）, }) [5 P% C4 I* k2 G" E9 I
Multilineage-differentiating stress-enduring (Muse)2 M$ e/ D& r6 O5 p
cells are a primary source of induced pluripotent7 W  ~$ m) Z8 M# p
stem cells in human fibroblasts
/ `, q5 M) _! t; WShohei Wakaoa,1, Masaaki Kitadaa,1, Yasumasa Kurodaa, Taeko Shigemotoa, Dai Matsusea, Hideo Akashia,- h8 c, O6 o5 o# N" Z9 H4 ?
Yukihiro Tanimuraa, Kenichiro Tsuchiyamaa,b, Tomohiko Kikuchia, Makoto Godac, Tatsutoshi Nakahatad,
6 r5 k1 h! A0 XYoshinori Fujiyoshic, and Mari Dezawaa,2
6 x3 ]# p, J4 `! g/ }: W: xDepartments of aStem Cell Biology and Histology, and bDermatology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8575,7 R' f' C. @( y& W2 [# U
Japan; cDepartment of Biophysics, Graduate School of Science, Kyoto Univerysity, Kyoto 606-8502, Japan; and dCenter for iPS Cell Research and Application,
! P& u. w4 N$ u  I1 T+ ZKyoto University, Kyoto 606-8501, Japan
% h* u8 Y! \6 |+ H! qEdited by Darwin J. Prockop, Texas A & M Health Science Center, Temple, TX, and approved May 4, 2011 (received for review January 19, 2011)" H0 i& f( {7 d
The stochastic and elite models have been proposed for the- I4 P* E& _- F; N
mechanism of induced pluripotent stem (iPS) cell generation. In1 v0 W* X, D7 z  t) `( k! t
this study we report a system that supports the elite model. We
2 r9 t5 N, d/ W  E% O) M5 jpreviously identified multilineage-differentiating stress-enduring' [# f/ A  m3 m4 g- p
(Muse) cells in human dermal fibroblasts that are characterized. n7 f' j; z! t& B
by stress tolerance, expression of pluripotency markers, selfrenewal,
) @8 \0 q" ?% f5 `! Oand the ability to differentiate into endodermal-, mesodermal-,
# Z0 d9 N; F  N$ }' a$ i- `and ectodermal-lineage cells from a single cell. They can
4 {) u+ \$ C8 i  Y6 ^" G; wbe isolated as stage-specific embryonic antigen-3/CD105 doublepositive) k9 l  e: ]' S8 K( ^
cells. When human fibroblasts were separated into Muse
( Z" I! `- U& n$ Z2 qand non-Muse cells and transduced with Oct3/4, Sox2, Klf4, and  C- E1 q  I7 L4 y% B2 h
c-Myc, iPS cells were generated exclusively from Muse cells but. ~2 Y9 Y6 l: [1 W
not from non-Muse cells. Although some colonies were formed; p- j8 F' I6 Z# S
from non-Muse cells, they were unlike iPS cells. Furthermore, epigenetic; [2 q) G5 p9 @: ?- t( G7 E
alterations were not seen, and some of the major pluripotency
! o+ \  G! B; E/ X( r- T* gmarkers were not expressed for the entire period during
; f- `. P5 m6 X$ k. k+ I% SiPS cell generation. These findings were confirmed further using
  \; ], C% O& o6 Ncells transduced with a single polycistronic virus vector encoding
$ M" }* u  k* L6 }7 Uall four factors. The results demonstrate that in adult human fibroblasts! t4 Z  s9 W/ B" I! F+ I
a subset of preexisting adult stem cells whose properties are
# [+ N" X; B  o9 Isimilar in some respects to those of iPS cells selectively become iPS1 |: U4 C" O; n  x& ~4 u. d
cells, but the remaining cells make no contribution to the generation0 A: b3 r4 n% N9 N' T, W- C
of iPS cells. Therefore this system seems to fit the elite model4 n" b; u0 G. ~% `8 v
rather than the stochastic model.
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( q2 W' ^7 L+ S: A' Y- e" l5 r在原始Fibroblast中用特定Marker分离出一群细胞，只有该种细胞可以被诱导为iPS，基本可以回答你的问题吧，MEF中可见一斑