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Tissue Eng. 2006 Sep;12(9):2695-706.. j0 \6 G5 _4 ~, M
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文章题目 Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels.  u& D& {; B: r5 E/ `1 \" p% o- L
Hwang NS, Varghese S, Zhang Z, Elisseeff J.1 r, Q5 P9 T2 z

% h% M$ R& S! v2 b) W* y- O- HDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21218, USA.
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( X2 X% {. m. ]2 H' H0 }* ]/ W5 nAbstract
) X& C" [8 X; gHuman embryonic stem cells (hESCs) have the potential to self-renew and generate multiple cell types, producing critical building blocks for tissue engineering and regenerative medicine applications. Here, we describe the efficient derivation and chondrogenic differentiation of mesenchymal-like cells from hESCs. These cells exhibit mesenchymal stem cell (MSC) surface markers, including CD29, CD44, CD105, and platelet-derived growth factor receptor-alpha. Under appropriate growth conditions, the hESC-derived cells proliferated without phenotypic changes and maintained MSC surface markers. The chondrogenic capacity of the cells was studied in pellet culture and after encapsulation in poly(ethylene glycol)-diacrylate (PEGDA) hydrogels with exogenous extracellular proteins or arginineglycine- aspartate (RGD)-modified PEGDA hydrogels. The hESC-derived cells exhibited growth factor- dependent matrix production in pellet culture but did not produce tissue characteristic of cartilage morphology. In PEGDA hydrogels containing exogenous hyaluronic acid or type I collagen, no significant cell growth or matrix production was observed. In contrast, when these cells were encapsulated in RGDmodified poly(ethylene glycol)hydrogels, neocartilage with basophilic extracellular matrix deposition was observed within 3 weeks of culture, producing cartilage-specific gene up-regulation and extracellular matrix production. Our results indicate that precursor cells characteristic of a MSC population can be cultured from differentiating hESCs through embryoid bodies, thus holding great promise for a potentially unlimited source of cells for cartilage tissue engineering.: e- j2 ?5 ~! s; t: L

) G/ G. c8 W  d1 R& b  ]& KPMID: 16995803 [PubMed - indexed for MEDLINE]

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