PDF电子书：RT-PCR Protocols 2nd ED 2010(MiMB 630)

jimmy88cn

% l% u6 T8 s5 u0 s6 ^

+ d6 v; m/ d9 b2010出版的 Methods in Molecular Biology 系列 vol.630
( ^" x4 K+ V0 X7 X& j7 r与本站朋友分享（请勿外传，以免引起不必要的版权纠纷)。5 X+ o/ _# K# A' q

5 u) d: w! K' g- p. P. b0 k: y* k, Y( Z4 q
RT-PCR Protocols
6 z) U) ^8 u' L$ Q( f0 ZSecond Edition
) p: k4 k- i+ G. ?: v/ `/ g8 |Edited by Nicola King- i- @5 p* q" y; V1 o2 f9 p( k
University of New England, School of Science and Technology, Armidale, Australia: w( a% o8 D' H  B" d3 B9 A
ISSN 1064-3745 e-ISSN 1940-6029+ u& u/ d  S+ A7 `
ISBN 978-1-60761-628-3 e-ISBN 978-1-60761-629-0
' z: q" N. \6 e7 d# I+ KDOI 10.1007/978-1-60761-629-0
6 O( ~+ O! W# M" Q3 i1 @- {# wSpringer New York Dordrecht Heidelberg London
8 h8 a( |' Y* i- s$ f0 Y7 NLibrary of Congress Control Number: 2010923597
6 k4 G4 j9 Z1 F7 ?9 l' s! W7 ^- j© Springer Science+Business Media, LLC 2010; C7 A, w/ ^3 Y7 g# N

1 X9 a, N$ X; z' g& HPreface$ u) \& z* q" `; t  E0 T
Thirty years ago, the investigation of gene sequences and, in particular, disease-causing; z3 T, q$ i) t; H4 |
mutations was a tedious, highly skilled operation; however, this field was revolutionized in* w7 Z+ A# ~& S( x
the mid-1980s with the introduction and subsequent development of a technique which  D3 M% M/ _2 @( H1 t
enabled the reliable amplification of minute quantities of starting DNA into readily detectable6 [& v4 F  F4 `( M
levels. Nowadays, this technique, otherwise known as reverse-transcription polymerase( F8 ?0 r6 S" N: S4 R) n$ J
chain reaction (RT-PCR), has become routine in most laboratories. Indeed, such
, G8 U( ~! I$ Cis the popularity of RT-PCR that a protocol has been published describing how this could
6 H3 [$ t6 K1 F8 ]3 wbe carried out in a kitchen using common household equipment. The aim of this volume2 s# n* i& ~$ v/ q! p) Q# B
is to translate RT-PCR theory into practice. To achieve this, a comprehensive guide to
9 U# W0 f# f- a. u- B2 M# Zcurrently available RT-PCR techniques is given in the form of user-friendly protocols.! d. s% P3 q1 _1 }' ~4 J+ L% a) B( r
These protocols contain precise information about all the necessary chemical, consumable,7 A+ U3 ^9 e9 z- S' ?9 P
and equipment resources and detailed instructions about how to perform each stage7 a) e- V" Q6 v& L& ~6 i
of the different methods. Furthermore, each protocol concludes with a comprehensive
& Y/ M7 M% L! C6 C- J) X, ^  unotes section, where authors provide helpful hints, trouble-shooting tips, and other mustknow+ g# x  w! z+ C# t/ d
information in a format which is accessible to the beginner.2 m8 G5 Y+ x4 X0 {7 r
RT-PCR protocols were the subject of an earlier edition in the Methods in Molecular: ]# S4 C6 P" H0 R0 T0 ]
Biology™ series, which was published in August 2002. In this second edition, some of the" N& T' s" E, z" B! L8 d8 L+ R3 f! A
contents of the previous edition are revisited bringing these technologies up to date, for5 v. h! q$ D/ C
example, competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR
  ?1 i' r$ H/ Y* m) s3 sfor cloning. In addition, the second volume also describes the newer technologies that have
' T8 [% w$ y" i* Qbeen developed and applied in the last 7 years including multiplex RT-PCR and RT-LATEPCR.
* `& x" _8 |5 R% k. l7 _This growth and development is reflected in the wide selection of basic RT-PCR5 A- }" R2 D+ Z3 C6 T3 {6 E! v
techniques which are presented in the first section entitled “The RT-PCR Detective: Hunting
9 H2 _+ r$ O5 |! u: }+ R$ ]Down the Right Method”. Arguably, however, the greatest advances in RT-PCR have come8 u& Y& o+ @: S0 N
in the field of real-time quantitative RT-PCR, and this, along with all the other means of9 I* S. Q$ E0 c6 D
quantifying PCR products, is explained in the second section, “The RT-PCR Mathematician:8 K7 \0 @- X% Z  P  i& k6 h( I
Assessing Gene and RNA Expression”. Finally, since designing RT-PCR experiments requires
. {8 u" S% y; D6 L+ U  g$ j' Tboth the correct recipe and the best ingredients, the last section, “The RT-PCR Master" i8 B3 P2 i7 I3 d, o
Chef: Finding the Best Ingredients,” is devoted to recent advances in some of the individual
; H% V2 d4 ^4 D+ @+ V) u3 o' u+ welements that go together to make the optimum RT-PCR reaction, e.g. RNA extraction,
* S; @  y7 y) m8 r/ uprimer design, and reverse transcription.0 d2 h/ w4 U3 R. f' e' |4 w
This volume is not intended to be a hard-core technical manual that is accessible to a
1 i, u$ W1 x' Dfew Nobel Laureate molecular biologists. Rather, the goal is that it should act as a handy. d6 J/ R9 d9 B2 m
companion to anyone who wants to explore the marvels of gene expression. This includes
% Y4 A; m- _0 U" e  nstudents and their tutors, researchers, laboratory managers, and technologists from many8 F% \+ p6 ]. d  h* Y, X
diverse disciplines ranging from biochemistry to zoology and forensics to physiology.
6 a/ z5 Z7 s  A* h+ z- Y  \. ~6 W7 x' {6 F. ~& T) S
Armidale, NSW . B2 q$ \( G. o; ]; ~  M
Nicola King) S( t# P2 Q; Y
: G- W& F1 Q" a$ t' V- I! @/ `- @
[hide][/hide]

ladybird

原来是这样  

安生

哈哈，看的人少，回一下  

apple0

看贴回复是好习惯  

www1202000

我的啦嘿嘿  

dr_ji

顶也～  

frogsays

不错，支持下  

dada

牛牛牛牛  

化药所

老大,我好崇拜你哟  

舒思

好帖，有才