PDF电子书：RT-PCR Protocols 2nd ED 2010(MiMB 630)

jimmy88cn

2 U) q9 N% F9 d
! D; m0 _8 M, F2 \' l- y: N
2010出版的 Methods in Molecular Biology 系列 vol.630
! P. U* F2 y+ D, h: C5 q; {1 @与本站朋友分享（请勿外传，以免引起不必要的版权纠纷)。
0 p  ?( g1 u, k# d# l
: u5 b) a% r  o5 V4 K
, o- z1 ]& `0 ?( u- H/ \8 D9 lRT-PCR Protocols2 ~5 V& c* D( ^5 q' z  l! q
Second Edition& C6 a  z  ?2 c5 m6 n3 z! L
Edited by Nicola King, I' r( x1 \- d* c
University of New England, School of Science and Technology, Armidale, Australia: h" c" [2 X1 ?$ Q/ q) B
ISSN 1064-3745 e-ISSN 1940-60297 m3 k8 Q: Z7 _+ N9 U0 y
ISBN 978-1-60761-628-3 e-ISBN 978-1-60761-629-0
6 O* C- u7 @: f$ t' BDOI 10.1007/978-1-60761-629-0
6 P. \9 m! b0 y* P* o3 ?( e  A1 [Springer New York Dordrecht Heidelberg London' C* p6 Q/ L2 E8 i% w
Library of Congress Control Number: 2010923597
" t" E+ n, o2 [. \% r© Springer Science+Business Media, LLC 2010  m3 V1 t! q4 }
0 R+ Z) E8 c9 X' l5 n
Preface" L2 i/ A! r" V/ ?
Thirty years ago, the investigation of gene sequences and, in particular, disease-causing
& C6 _! L2 n1 {, m) x; L. w, Ymutations was a tedious, highly skilled operation; however, this field was revolutionized in
0 W+ q; X1 P; n) \6 ]the mid-1980s with the introduction and subsequent development of a technique which! X) H' u5 s2 j9 \0 [
enabled the reliable amplification of minute quantities of starting DNA into readily detectable5 s3 p" L0 j3 {
levels. Nowadays, this technique, otherwise known as reverse-transcription polymerase
: @, z+ H( @% L' lchain reaction (RT-PCR), has become routine in most laboratories. Indeed, such$ K  S3 K  U2 L$ m% M5 f  a
is the popularity of RT-PCR that a protocol has been published describing how this could8 g2 a* d% z- g8 y# ]  [, V1 c% `
be carried out in a kitchen using common household equipment. The aim of this volume* A" O) H, ~: z; Z0 E: e
is to translate RT-PCR theory into practice. To achieve this, a comprehensive guide to
* m) {- Y/ Q* Z% B+ g( Wcurrently available RT-PCR techniques is given in the form of user-friendly protocols.' r0 u7 f1 p( u* S( h; `6 g
These protocols contain precise information about all the necessary chemical, consumable,
7 ?0 ^3 }1 W. L* T8 Iand equipment resources and detailed instructions about how to perform each stage7 h% D* h( \5 ~2 b8 h: _7 h
of the different methods. Furthermore, each protocol concludes with a comprehensive
8 |) a( v' N" knotes section, where authors provide helpful hints, trouble-shooting tips, and other mustknow9 s% Y7 S! m4 [7 a0 j$ a
information in a format which is accessible to the beginner.
; s* O; k) @2 BRT-PCR protocols were the subject of an earlier edition in the Methods in Molecular
9 ]! a. \& w: ^/ q) H# UBiology™ series, which was published in August 2002. In this second edition, some of the
9 z  u# s# M7 I' _6 Gcontents of the previous edition are revisited bringing these technologies up to date, for
' Q, Y9 S: I" \* y* l( H- y: eexample, competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR
- I  O: t( i; X9 x# H9 U+ ~for cloning. In addition, the second volume also describes the newer technologies that have
" g' g5 T$ ?. Q; U2 w$ _* D- d$ zbeen developed and applied in the last 7 years including multiplex RT-PCR and RT-LATEPCR.
8 ~" Z4 D2 L! B  xThis growth and development is reflected in the wide selection of basic RT-PCR
1 K4 D5 ~& D+ p0 E% q% `! N- ]techniques which are presented in the first section entitled “The RT-PCR Detective: Hunting
1 z( u* }* L1 D) p. ~Down the Right Method”. Arguably, however, the greatest advances in RT-PCR have come
# u2 N1 l( X& r: rin the field of real-time quantitative RT-PCR, and this, along with all the other means of
' ?0 {  r+ O$ w/ G8 [+ v* N) Bquantifying PCR products, is explained in the second section, “The RT-PCR Mathematician:
' Q# r1 `: ~, [! ^Assessing Gene and RNA Expression”. Finally, since designing RT-PCR experiments requires
/ \4 I8 l1 [$ }9 ^8 F4 N/ Nboth the correct recipe and the best ingredients, the last section, “The RT-PCR Master
0 r2 Z4 c* Z. E1 e' }Chef: Finding the Best Ingredients,” is devoted to recent advances in some of the individual
7 t' W8 ?3 S; V' {, Celements that go together to make the optimum RT-PCR reaction, e.g. RNA extraction,$ R; a0 @. z! w! P" A7 k, w
primer design, and reverse transcription.
' q& e( G5 [, I1 d" j1 d, ^5 q3 _This volume is not intended to be a hard-core technical manual that is accessible to a$ O% l! O' Q: s
few Nobel Laureate molecular biologists. Rather, the goal is that it should act as a handy8 t/ s: f5 c* y0 h6 ]8 L/ J" M! w, u
companion to anyone who wants to explore the marvels of gene expression. This includes9 B. J/ y, r6 Y+ H0 y. B( T
students and their tutors, researchers, laboratory managers, and technologists from many7 A/ _# N+ e& {2 w5 L
diverse disciplines ranging from biochemistry to zoology and forensics to physiology.
' F  _. O; ^) i7 T" Y# ^/ s9 w
9 \2 L; r4 b* R5 H7 g% x* }/ |Armidale, NSW
$ i. h# k8 q3 j) F, a- X5 ~Nicola King9 T& N' A" N; Y8 l6 ]1 t
$ l5 P; p, p. l
[hide][/hide]

ladybird

原来是这样  

安生

哈哈，看的人少，回一下  

apple0

看贴回复是好习惯  

www1202000

我的啦嘿嘿  

dr_ji

顶也～  

frogsays

不错，支持下  

dada

牛牛牛牛  

化药所

老大,我好崇拜你哟  

舒思

好帖，有才