PDF电子书：RT-PCR Protocols 2nd ED 2010(MiMB 630)

jimmy88cn

2 a, r& n0 J) F7 y  r* ]* u2 i& I, N/ |( J) Z
2010出版的 Methods in Molecular Biology 系列 vol.630
' J( c; x3 y3 }! S, ~# O与本站朋友分享（请勿外传，以免引起不必要的版权纠纷)。
% x* r4 o$ l6 O- O6 M4 U! X, M9 ?3 r' e" N

( G, C, {% C8 L2 O7 ~$ |5 uRT-PCR Protocols$ v/ {: A, m& K8 T) d5 n: Q8 T. [
Second Edition' Q  I: v( @0 B0 N9 ?' t( @/ N
Edited by Nicola King! ?8 \. {9 t) H- _. |: k7 M
University of New England, School of Science and Technology, Armidale, Australia
9 o3 ^2 ~7 j. Z! pISSN 1064-3745 e-ISSN 1940-6029( c4 m0 K0 g' X$ i. m8 I' q
ISBN 978-1-60761-628-3 e-ISBN 978-1-60761-629-0
$ p$ n2 P; @6 V4 zDOI 10.1007/978-1-60761-629-0/ J8 s" j7 y) G9 h# h  [* H
Springer New York Dordrecht Heidelberg London
8 p, L! p0 U8 X0 l' D; hLibrary of Congress Control Number: 20109235979 K$ \, q: V4 U& E. _& X% t
© Springer Science+Business Media, LLC 2010
2 O4 p6 b( j  \6 T; Y% s! o4 L4 H/ V
Preface
. _$ `6 d  C8 {1 Q! j" bThirty years ago, the investigation of gene sequences and, in particular, disease-causing
/ H6 D2 ?' u0 }) wmutations was a tedious, highly skilled operation; however, this field was revolutionized in- |- }# D8 h( j1 W: ^4 U
the mid-1980s with the introduction and subsequent development of a technique which, z% C' \: _, j' s7 r# y& f0 n
enabled the reliable amplification of minute quantities of starting DNA into readily detectable
' C, S- p* K- U, F( v9 s% ulevels. Nowadays, this technique, otherwise known as reverse-transcription polymerase( I1 i/ _. F1 G5 D* w
chain reaction (RT-PCR), has become routine in most laboratories. Indeed, such$ t- ^: S; j) Y- f! k; U
is the popularity of RT-PCR that a protocol has been published describing how this could
+ U$ X5 s$ D! `$ Q8 Lbe carried out in a kitchen using common household equipment. The aim of this volume
: K, X5 {- h/ g( J! Xis to translate RT-PCR theory into practice. To achieve this, a comprehensive guide to
# R* x5 g4 Q- U+ \currently available RT-PCR techniques is given in the form of user-friendly protocols.
9 z: \7 [4 t/ Y, L/ d$ tThese protocols contain precise information about all the necessary chemical, consumable,1 e& A7 o( p; {
and equipment resources and detailed instructions about how to perform each stage  H' U0 T+ n0 R  }
of the different methods. Furthermore, each protocol concludes with a comprehensive
. v( `% v, G. J4 z# x  {notes section, where authors provide helpful hints, trouble-shooting tips, and other mustknow
0 y' D# n8 h! G3 n9 l% @& cinformation in a format which is accessible to the beginner.- O; S. X' e; C+ ^$ m
RT-PCR protocols were the subject of an earlier edition in the Methods in Molecular" [# X1 l; q& h5 {
Biology™ series, which was published in August 2002. In this second edition, some of the
3 ^7 U! b" U# x8 y1 N! rcontents of the previous edition are revisited bringing these technologies up to date, for: ]) V: ~5 _* t  f$ b
example, competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR
  G% _1 n% b$ V# afor cloning. In addition, the second volume also describes the newer technologies that have7 r# ^3 O; L5 |% ~9 u! d0 f9 @2 s5 T
been developed and applied in the last 7 years including multiplex RT-PCR and RT-LATEPCR.; k3 J+ M# u* O1 B& m% w- ~* v5 d9 K
This growth and development is reflected in the wide selection of basic RT-PCR
$ |1 c4 z) P3 `  }6 Otechniques which are presented in the first section entitled “The RT-PCR Detective: Hunting. u, k( g" {. _2 J1 Z
Down the Right Method”. Arguably, however, the greatest advances in RT-PCR have come1 w: g; ?( G# Z# H" r
in the field of real-time quantitative RT-PCR, and this, along with all the other means of
. i2 Q5 T9 I$ s. s3 f2 s0 Hquantifying PCR products, is explained in the second section, “The RT-PCR Mathematician:2 [. p6 p1 a/ s# n& ^/ v2 \9 V
Assessing Gene and RNA Expression”. Finally, since designing RT-PCR experiments requires$ h/ y4 o7 R3 B
both the correct recipe and the best ingredients, the last section, “The RT-PCR Master8 Y5 A# t0 ^: t
Chef: Finding the Best Ingredients,” is devoted to recent advances in some of the individual  m9 d, G' z* u( j2 E4 o2 b
elements that go together to make the optimum RT-PCR reaction, e.g. RNA extraction,9 a- W# K' v: R/ Q6 z9 p
primer design, and reverse transcription.
: ~/ ?* a/ x% o) `1 ^- q: m: X$ P2 hThis volume is not intended to be a hard-core technical manual that is accessible to a
1 I  D$ i! y# h& l6 f# _! o( \0 jfew Nobel Laureate molecular biologists. Rather, the goal is that it should act as a handy
3 `+ o/ E2 s9 q5 D; c# M* ?' hcompanion to anyone who wants to explore the marvels of gene expression. This includes, u1 n$ Z' H9 @; Z+ N/ ^
students and their tutors, researchers, laboratory managers, and technologists from many! }( a9 p- B/ }3 ^5 `
diverse disciplines ranging from biochemistry to zoology and forensics to physiology.- V# e( e& g6 a0 x
, b% ^6 n# ^& W8 X
Armidale, NSW
8 L& V  ^: }% N7 JNicola King4 w2 {; R: L2 o( ^) G3 W

0 |: i4 u/ R1 A0 t! t[hide][/hide]

uyunbilig

谢谢分享

wzx6660

谢谢分享

maomaoma

谢谢分享！

woosheng

谢谢

flyingpnmc

谢谢

liangyinxie

回复 1# jimmy88cn
1 C  ~+ \( \- z8 ~( U5 v- z1 ]2 G. C/ W

/ T. k- q4 e* P: p+ i    thank you

maxmad

先谢谢！！支持！！！

maxmad

支持！！！！！正需要！！

angel-sakura

谢谢~~~过来支持下~