Islet 1心脏祖细胞可以发育为血管内皮细胞、心肌细胞、窦房结细胞

zhangjinxy

Dev Biol. 2007 April 1; 304(1): 286–296.1 y: }" E* B, c
    Islet1 (Isl1) is a LIM homedomain protein that plays a pivotal role in cardiac progenitors of the second heart field. Here, lineage studies with an inducible isl1-cre demonstrated that most Isl1 progenitors have migrated into the heart by E9. Although Isl1 expression is downregulated in most cardiac progenitors as they differentiate, analysis of an isl1-nlacZ mouse and coimmunostaining for Isl1 and lineage markers demonstrated that Isl1 is expressed in distinct subdomains of the heart, and in diverse cardiovascular lineages. Isl1 expression was observed in myocardial lineages of the distal outflow tract, atrial septum, and in sinoatrial and atrioventricular node. The myocardialized septum of the outflow tract was found to derive from Isl1 expressing cells. Isl1 expressing cells also contribute to endothelial and vascular smooth muscle lineages including smooth muscle of the coronary vessels.) C* R. Z: o) w$ {' ]( d3 J
      Our data indicate that Isl1 is a specific marker for a subset of pacemaker cells at developmental stages examined, and suggest genetic heterogeneity within the central conduction system and coronary
3 g0 g. h9 w3 `, Zsmooth muscle. Our studies suggest a role for Isl1 in these distinct domains of expression within the heart.

junjunxu

心肌干细胞的分离与培养，方法？楼主是否有，谢谢！

zhangjinxy

抱歉，现在准备阶段，还没开始动手做实验

zhangjinxy

抱歉，现在准备阶段，还没开始动手做实验

xuhuanyu

共同学习
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