最新iPS诱导肝脏再生又出新成果！（2010年8月25）

shihuijunm

3 Q8 O7 T) z& U) T( f  j, BTitle：Induced pluripotent stem cell–derived hepatocytes have the functional and proliferative capabilities needed for liver regeneration in mice  s# k' @; Z* y! s7 _) N4 J  U
Author：Silvia Espejel, Garrett R. Roll, K. John McLaughlin,Andrew Y. Lee,Jenny Y. Zhang,  Diana J. Laird, Keisuke Okita,Shinya Yamanaka, and Holger Willenbring) R! t, b) T( D8 x* W8 `
Abstract：The ability to generate induced pluripotent stem (iPS) cells from a patient’s somatic cells has provided a foundation for organ regeneration without the need for immune suppression. However, it has not been established that  the differentiated progeny of iPS cells can effectively reverse failure of a vital organ. Here, we examined  whether iPS cell–derived hepatocytes have both the functional and proliferative capabilities needed for liver regeneration in mice with fumarylacetoacetate hydrolase deficiency. To avoid biases resulting from random genomic integration, we used iPS cells generated without viruses. To exclude compensation by hepatocytes not derived from iPS cells, we generated chimeric mice in which all hepatocytes were iPS cell derived. In vivo analyses showed that iPS cells were intrinsically able to differentiate into fully mature hepatocytes that provided full liver function. The iPS cell–derived hepatocytes also replicated the unique proliferative capabilities of normal hepatocytes and were able to regenerate the liver after transplantation and two-thirds partial hepatectomy. Thus, our results establish the feasibility of using iPS cells generated in a clinically acceptable fashion for rapid and stable liver regeneration.

shihuijunm

shihuijunm

补充相关文献一篇 没来的及看 都是新的 大家学习下
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shihuijunm

回复 10# sannia
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    我也是新手 不是做肝脏的 准备做动物方面的iPS,才刚开始接触这一领域，还没多少头绪呢 ，向大家学习~