日本科学家发布---“胚胎干细胞和人工多能干细胞”诱导分化的方法（附日文原文）

hyde

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8 \1 {; L' ]  OMETHOD FOR INDUCING DIFFERENTIATION OF EMBRYONIC STEM CELLS OR ARTIFICIAL PLURIPOTENT STEM CELLS3 ^) p3 [0 ~1 h1 X( s& U
http://www.wipo.int/pctdb/en/wo. ... &DISPLAY=STATUS* v% A; O+ {+ m( G+ w
Abstract:        & n5 p6 @! l/ L9 J& i+ k4 |9 c9 e$ p/ i
(EN) Differentiation of large volumes of embryonic stem cells or artificial pluripotent stem cells can be easily induced by the following method. Using a substrate surface wherein there is a cell nonadhesive region enclosing a cell adhesive region, (1) cells are caused to adhere to the cell adhesive region on the substrate surface under a differentiation-inducing medium, (2) then the cells are propagated on the cell adhesive surface until confluency is reached, (3) culture is continued further such that the cells form three-dimensional layers from the boundary with the cell nonadhesive region enclosing the cell adhesive region, and (4) the multiple layered portions that have formed inside the cell adhesive region are allowed to eventually merge such that the entire cell adhesive region creates a thick cell aggregate.: ~1 c' n% t! z, r& G$ ]/ Q& e1 T

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! O- U. n+ E) `+ |. @2 |4楼原文 感谢woosheng 提供

FYH

有文章吗？谢谢　

hyde

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暂时没有原文
1 _5 l# R* x) k) t" w4 ^是通过google的邮箱订阅获得信息，没有搜到原文^^^
6 I: s- m4 A! Y" O还没理解摘要的意思，求解释？

woosheng

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& W2 R0 G# B! n7 `5 A9 G( y5 R  V这是一篇专利，原文是日文。
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deron

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厄，，原文有没有人会翻译？……

deron

大量胚胎干细胞或者人工诱导多功能干细胞的分化可以简单概括为以下方法6 F/ x# B! h; E5 D/ A: c! I
用一个底表面——一个不具有细胞黏附性的区域环绕一个具有细胞黏附性的区域做成的底表面，2 j4 h! {/ l, b5 P$ H
（1）细胞在分化诱导培养基作用下在黏附性区域贴壁
  l/ |$ D3 |, Q$ e（2）随后细胞就在细胞黏附区域繁殖至到达克隆8 ~5 ]  \; H9 \
（3）继续培养致使细胞在与周围非黏附性区域交界处形成三维立体结构层
2 q% q6 w& p6 t  A6 N. |' R9 Y! C! a（4）在黏附性区域内部形成的多层部分可以最终融合使得整个细胞黏附区域形成一个厚的细胞集合。9 V( \* y" ~/ O1 _) [
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没做过分化，还真是搞不明白……

bypw

这是篇日文啊，看不懂啊，有没有英语的啊