
- 积分
- 198
- 威望
- 198
- 包包
- 761
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Differentiation of mouse embryonic stem cells and of human adult stem cells into adipocytes.
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2 k" t {. y/ S! Y3 B/ A" m8 W+ AWdziekonski B, Villageois P, Dani C.7 d* M. m5 e8 c5 E
- e/ T) r+ _. ], I, N) b' V0 oCNRS Université de Nice Sophia Antipolis, Nice, France.
- |% X# j5 a2 ]+ o' W3 sAbstract) |: k/ d) e! Q; V& S7 b
The authors describe protocols for culture conditions in which mouse ES cells can be maintained in an undifferentiated state or committed to undergo adipocyte differentiation at a high rate and in a highly reproducible fashion. There is also a protocol for maintaining and differentiating human adult stem cells, isolated form adipose tissue and from bone marrow, into adipocytes. These culture systems provide a powerful means for studying the first step of adipose cell development and a means to investigate effects of drugs on the biology of adipocytes. There are also protocols for detection of adipocytes and analysis of their gene expression. |
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