调节表达microRNAs-126/126*能抑制HES形成血细胞

hyde

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Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cells.% z) ]4 \2 G, B/ t% @6 f) i: @* Z
Huang X, Gschweng E, Van Handel B, Cheng D, Mikkola HK, Witte ON.  ^* _7 w% X; E/ {- j
Howard Hughes Medical Institute, UCLA, Los Angeles, United States;
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# C, {( {9 h! \1 d" M  e说明：原文来源自
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6 J1 B+ o% s7 V" ^1 C1 i. {http://bloodjournal.hematologyli ... od-2010-08-302711v1# q! A3 k2 S- D- ~8 e) E' F
由干细胞之家新闻小组成员hyde翻译（转帖请注明）8 e+ L% u4 S7 |% @

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miRs在细胞分化和细胞特性的决定中扮演了重要的角色，但是到目前为止，关于其对人类造血细胞系诱导分化的作用仍然知之甚少。hESCs提供了一个模型系统用于研究人类早期的造血过程。我们将hESCs诱导形成胚体（此过程不懂请指教），比较CD34(+) EB cells（胚体中的造血干细胞）和未分化hESCs的miR表达情况。在CD34(+) 细胞中上调的七种 miRs中MiRs-126/126*富集最多。它们的表达与造血转录因子RUNX1, SCL and PU.1的动力学相关联。为了明确MiRs-126/126*在血细胞生成中的作用，我们改造了hESC使其过表达与强力霉素关联的miRs-126/126*（不懂？），分析它们的血细胞分化过程。在胚体形成分化和克隆体形成的过程中诱导miRs-126/126*，降低了红细胞生成相关的细胞克隆，这表明在红细胞形成中miRs-126/126*起到了抑制性的作用。PTPN9蛋白酪氨酸磷酸酶，与红细胞生成相关细胞克隆的生长和扩增有密切的关系，是miR-126的一个靶点。对PTPN9进行修复可以部分缓解 miRs-126/126*.造成的对血细胞生成的抑制性作用。我们的研究结果表明miRs-126/126*对于血细胞的形成起到了负面的调节作用，首次证明了miR在hESCs造血分化中的作用。
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MicroRNAs (miRs) play an important role in cell differentiation and maintenance of cell identity, but relatively little is known of their functional role in modulating human hematopoietic lineage differentiation. Human embryonic stem cells (hESCs) provide a model system to study early human hematopoiesis. We differentiated hESCs by embryoid body (EB) formation and compared the miR expression profile of undifferentiated hESCs to CD34(+) EB cells. MiRs-126/126* were the most enriched of the seven miRs that were up-regulated in CD34(+) cells, and their expression paralleled the kinetics of hematopoietic transcription factors RUNX1, SCL and PU.1. To define the role of miRs-126/126* in hematopoiesis, we created hESCs overexpressing doxycycline-regulated miRs-126/126* and analyzed their hematopoietic differentiation. Induction of miRs-126/126* during both EB differentiation and colony formation reduced the number of erythroid colonies, suggesting an inhibitory role of miRs-126/126* in erythropoiesis. PTPN9, a protein tyrosine phosphatase that is required for growth and expansion of erythroid cells, is one target of miR-126. PTPN9 restoration partially relieved the suppressed erythropoiesis caused by miRs-126/126*. Our results define an important function of miRs-126/126* in negative regulation of erythropoiesis, providing the first evidence for a role of miR in hematopoietic differentiation of hESCs.8 q/ Z" M2 Y6 M0 u# ?2 w' b2 f7 w5 N/ A
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求原文？, l* a: G  P9 S, V
有些过程机理不懂所以语言不谢欠妥当。
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6楼原文 感谢hansey 提供

hyde

那个MiRs-126/126* were the most enriched 是不是未分化的细胞中的现象？

ndwzf

very good

xiaxin1900

求原文，谢谢

xiaxin1900

谁有原文啊，拜托发一份，十分感谢。邮箱373657996@qq.com

hansey

原文：