多能细胞中HIV-1整合位点偏好性的研究

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基于HIV-1的载体已被广泛应用到基因治疗中。在体细胞中，这些载体大部分整合到基因内，然而，还没有观察到明显的整合位点在打的染色体区域上的偏好性。最近，类似与胚胎干细胞（ES）的诱导多能干细胞（iPS）的出现是人们产生了在这些细胞中载体整合位点到底在哪里的疑问。这项研究中我们研究了基于HIV-1的载体在多能的ES样细胞中的整合位点的偏好性。我们发现大约30%的整合发生在端粒附近。我们分析了很多种体细胞的整合位点，根据我们和其他小组的报导，这种整合模式只在多能细胞系中存在。我们推断多能细胞可能存在独特的参与靶向整合的细胞内辅因子，而这些辅因子在体细胞内并不存在。  j' v$ \1 F1 M8 ^( M+ ?

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HIV-1 integration site preferences in pluripotent cells
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HIV-1-based vectors are widely used in gene therapy. In somatic cells, these vectors mainly integrate within genes. However, no distinct integration site preferences have been observed with regard to large chromosomal regions. The recent emergence of induced pluripotent stem (iPS) cells, similar to embryonic stem (ES) cells, has raised questions about where integration occurs in these cells. In this work we investigated the integration site preferences of HIV-1-based vectors in a pluripotent, ES-like cell line. We show that approximately 30% of the integrations occur in the vicinity of telomeres. We have analyzed integration sites in various somatic cells, as reported by us and other groups, and observed that this integration pattern is unique to the analyzed pluripotent cell line. We conclude that pluripotent cells may contain distinct cellular cofactors that participate in integration targeting and that are not present in somatic cells.
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# v1 V2 b: i8 V8 Vhttp://www.ncbi.nlm.nih.gov/site ... p;dopt=abstractplus9 u! I8 r6 v( ^, |5 O( s* I
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