求1988年Nature Biotechnology文章一篇

zlx0814

Nature Biotechnology 16, 642 - 646 (1998) 2 {' G2 n0 C0 A
doi:10.1038/nbt0798-6425 g3 }2 F. ]+ c& h  w( r  Z
  S# c$ o7 `3 M( d8 J" O: U8 c
: q$ n1 [  o' J/ j; O
Trasgenic bovine chimeric offspring produced from somatic cell-derived stem-like cellsJose B. Cibelli1, ,2, Stevem L. Stice2, Paul J. Golueke2, Jeff J. Kane2, Joseph Jerry1, Cathy Blackwell2, F. Abel Ponce de León2, ,3 & James M. Robl1, ,2, ,*! p$ b% a" W1 u/ V) B/ S1 K$ L; p

- p0 i, G; K  x8 [1 z; E
: x5 X  ~  t0 V8 }/ E--------------------------------------------------------------------------------
  A5 r2 u( n( ~& x& _! Z/ U# i, Y6 \$ _. g+ u
Abstract  We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endo-derm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.
' U0 c2 U+ v9 R
( v, L: u/ w: S+ X7 L 全文链接
7 r2 l% c) D' F* i6 Whttp://www.nature.com/nbt/journal/v16/n7/abs/nbt0798-642.html

inankai

Trasgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells
5 f6 K: n! O# ]' z0 r& ~) M1998

zlx0814

Thanks.