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本帖最后由 inankai 于 2011-3-10 11:26 编辑
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* v& C+ u8 f# W' iSolubility /Stability:
- Y8 b: S4 h, ?6 m( ]* q& ORetinol is practically insoluble in water or glycerol. It is soluble in absolute ethanol, methanol, chloroform, ether, fats and oils.7 Stock solutions of Retinol (1 mg/ml) were prepared in ethanol, diluted in DMSO under low light conditions and stored at -50°C under nitrogen in brown glass vials.11 Retinol solutions (50 uM) were sterile filtered before use.11 Retinol both as a solid and in solution is readily oxidized in air and inactivated by UV light. To reduce photodestruction of Retinol, manipulations of Retinol solutions can be performed under yellow or red light.12,13 Solutions may be stabilized by dissolving in oil, by the addition of anti-oxidant compounds including α-tocopherol, BHT or hydroquinone or by conversion to the palmitate and acetate esters.3, 22 It is recommended to prepare solutions fresh for optimal quality. However, if absolutely necessary, store solutions in the dark under an inert atmosphere at least at - 20°C preferably at -70°C. Solvents preferred for storage are peroxide-free ethyl ether, acid-free acetone or ethyl acetate. For short term storage, ethanol is suitable as a solvent for spectroscopic analysis.4 Newest stability studies showed that air contact is the most influential factor of loosing actity (highly air-sensitive), after 2 hours exposition to air about 1⁄4 quarter of the activity is lost. Daylight and water decrease as well the activity but with less strong impact. The temperature was not such important factor it was observed that sample could stay for several days at room temperature without remarkable loss of activity.& F. r5 u- B9 J, ~8 `$ B& d
* A* G, Z. X9 Z. g$ L1 c
附件是Sigma的说明。9 Q; u& h" S1 N1 Y
拿到任何药物，首先要做的是把说明书读上3遍。如果没有说明书，sigma的datasheet是最好的指南。 |
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发表于 2011-3-9 12:36
