体内细胞直接重编程包括离散过度和非多能性步骤

hyde

Direct in vivo cellular reprogramming involves transition through discrete, non-pluripotent steps) b' R+ B5 a2 T: d6 e( f+ Y  ?
原文来自于http://dev.biologists.org/content/138/8/1483
) O- O; c0 j0 g; z3 s由干细胞之家hyde，转帖请注明……  D, t+ y; w: \% f8 `4 ^2 \
总结：
6 K& A7 ^& j# {* D1 C7 u8 n  i/ ]/ E正常发育机体的细胞在组织损伤、特定人为处理或癌症等疾病过程中会发生性质上的变化。显然，在这个过程中除了组织细胞重编程成为ESC样细胞，还有组织细胞直接从一种组织细胞转变为另一种已知的组织细胞（have been described？）。直接进行细胞类型的转变为实施细胞治疗提供了一种可选的策略。但是我们对于细胞在直接转变过程中实际的变化过程，以及细胞直接转变后可能对组织器官造成的影响知之甚少。我们借助了自然情况下直接重编程的一个体内单细胞系统，在这个系统中一个C. elegans rectal cell 直接转变为一个motoneuron。我们深入分析了该过程中涉及到的细胞转变。我们发现在体内直接重编程的过程中重编程细胞通过中间形态进行转移。我们发现并研究了保守COE（？）转录因子UNC-3的一个突变体，这个突变体的细胞类型转变功能被抑制。我们确定细胞首先要完全消除最初的身份，然后逐步发生UNC-3依赖的转变过程并最终形成motoneuron。另外，与体外诱导重编程不同的是在体内自然条件下细胞逆转成一种失去分化能力的细胞（组织细胞）不会导致细胞潜力的增加（？）。我们的发现表明，细胞类型的直接转变是逐步完成的，而且这个去分化的过程能够在无细胞分裂的情况下发生。另外我们的结果表明体内重编程的机制能够恰当地限制细胞地潜能（防止细胞癌化……），这一发现对于再生医学有着重大的意义。* i: ?8 {# A* _3 ^3 x
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Summary
2 i/ L& [5 A  l( }$ j, [Cells can change identity during normal development, in response to tissue damage or defined artificial treatments, or during disease processes such as cancer. Strikingly, not only the reprogramming of tissue cells to an embryonic stem cell-like state, but also the direct conversion from one cell type to another have been described. Direct cell type conversion could represent an alternative strategy for cellular therapies. However, little is known about the actual cellular steps undertaken by a cell as it changes its identity and their possible consequences for the organism. Using an in vivo single-cell system of natural direct reprogramming, in which a C. elegans rectal cell transforms into a motoneuron, we present an in-depth analysis of the cellular transformations involved. We found that the reprogrammed cell transits through intermediate states during direct in vivo reprogramming. We identified and characterised a mutant in the conserved COE transcription factor UNC-3 in which this cellular transformation is blocked. We determined that complete erasure of initial identity first takes place, followed by stepwise, unc-3-dependent, redifferentiation into a motoneuron. Furthermore, unlike in vitro induced reprogramming, reversion to a dedifferentiated identity does not lead to an increase in cellular potential in a natural, in vivo context. Our findings suggest that direct cell type conversion occurs via successive steps, and that dedifferentiation can occur in the absence of cell division. Furthermore, our results suggest that mechanisms are in place in vivo to restrict cell potential during reprogramming, a finding with important implications for regenerative medicine. 4 u6 I% ^. I1 O1 ~" z1 X; [
有错勿怪，多多指正……并求原文……

sunsong7

谁可以提供一下原文？

xyklmu

回复 sunsong7 的帖子# z2 d6 D/ C4 t

9 I& P) G. X* q  gDirect in vivo cellular reprogramming involves transition through discrete, non-pluripotent steps78 ~. V, X+ [+ L
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原文如下：
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dabai22222

abstract有点意思，下来看看，谢了