间充质干细胞的冻存——低DMSO、无血清

deron

主题：应用低浓度DMSO和明确组分冻存液对人骨髓间充质干细胞的冻存4 `5 x6 S' C  R0 V7 `! V

* e+ c/ M! S: ~8 C) W) K说明：原文来源自http://onlinelibrary.wiley.com/doi/10.1002/btpr.464/abstract，由干细胞之家新闻小组成员deron翻译（转帖请注明）。% n0 w1 K6 W3 U

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本研究致力于研究定义明确的无血清冻存液（DMSO浓度水平7.5%、5%、2.5%依次降低），与聚乙烯乙二醇（PEG）或海藻糖联合应用于人骨髓来源的间充质干细胞（hBMSCs）冻存的可行性。hBMSCs是细胞治疗与组织工程干细胞应用的主要来源。此次试验应用的是约1°C/min的标准实验室冷冻方案，并探究了1,2-丙二醇在hBMSCs冻存中的效用。我们测量了解冻后细胞活性和早期凋亡情况、细胞代谢活性和生长动力，并在冻存后检测了细胞形态和向成骨、脂肪及软骨分化的能力。结果显示在7.5%DMSO、2.5%PEG和2%牛血清白蛋白中（冻存后）解冻的hBMSCs，与常规10%DMSO解冻的细胞对比，活性分别是82.9 ± 4.3% 和82.7 ± 3.7%。另外，5% DMSO+5% PEG和7.5% 1,2-丙二醇+2.5% PEG 在有2%白蛋白存在时，对hBMSCs的冻存效果更好。在向所有检测冻存液中加入白蛋白之后，我们观察到了细胞活力的增强。
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6 L- t: t" E8 L7 I; J原摘要：The aim of this study is to investigate the feasibility of using well defined, serum-free freezing solutions with a reduced level of dimethylsulfoxide (DMSO) of 7.5, 5, and 2.5% (v/v) in the combination with polyethylene glycol (PEG) or trehalose to cryopreserve human bone marrow-derived mesenchymal stem cells (hBMSCs), a main source of stem cells for cell therapy and tissue engineering. The standard laboratory freezing protocol of around 1°C/min was used in the experiments. The efficiency of 1,2-propandiol on cryopreservation of hBMSCs was explored. We measured the post-thawing cell viability and early apoptotic behaviors, cell metabolic activities, and growth dynamics. Cell morphology and osteogenic, adipogenic and chondrogenic differentiation capability were also tested after cryopreservation. The results showed that post-thawing viability of hBMSCs in 7.5% DMSO (v/v), 2.5% PEG (w/v), and 2% bovine serum albumin (BSA) (w/v) was comparable with that obtained in conventional 10% DMSO, that is, 82.9 ± 4.3% and 82.7 ± 3.7%, respectively. In addition, 5% DMSO (v/v) with 5% PEG (w/v) and 7.5% 1,2-propandiol (v/v) with 2.5% PEG (w/v) can provide good protection to hBMSCs when 2% albumin (w/v) is present. Enhanced cell viability was observed with the addition of albumin to all tested freezing solutions.
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原文链接：http://onlinelibrary.wiley.com/doi/10.1002/btpr.464/abstract（求原文）

deron

现阶段细胞的冻存液的配制主要基于DMSO+血清+培养基，众所周之DMSO对细胞是有毒副作用的，而血清的组分复杂，对细胞有不可预知性的影响，因而本文的低DMSO和无血清（但是有白蛋白）很有噱头，自然就惹人注目。

sunsong7

很有价值的工作，求原文

农夫有点田

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学习了 多谢了

tjutiger

求原文，谢谢！！！

deron

偶的叮当……
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wxdang

谢谢分享啊