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本帖最后由 jun8013 于 2011-4-12 23:22 编辑 0 `# U/ s' J4 E; G: g) L
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3 Z( c& ?1 U6 Y8 `+ o8 fSSEA-1：CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.SSEA-4: The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.
; w5 o/ m3 |) n$ t( m; g4 D% ZTRA-1-81: The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 (Tumor Rejection Antigen 1-81) on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.0 P( J; z3 h; C) T7 a
CD338: CD338, known as ABCG2 (BCRP1), is a multi-drug resistance (MDR) family protein that is a member of ATP binding cassette (ABC) transporters. It is highly expressed on primitive ‘sidepopulation’ (SP) stem cells. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. The SP cells express low or undetectable levels of CD34. In the bone marrow, about 0.05% population of cells displays the low fluorescence and are highly enriched for repopulating cells. The highest levels of BCRP1 mRNA expression have been seen in KDR+ human stem cells. The expression of ABCG2 appears to be highly conserved as it has been identified in various species.) y9 L" p& t& T
CD49f: CD49f is a 120 kD integrin family member, also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin α6 function in vivo.
. S r5 i/ H7 G! j7 r; o4 ~CD324: The 67A4 antibody recognizes human CD324 also known as E-cadherin, cadherin-1, and UVO. CD324, a member of the cadherin superfamily, is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region with a predicted molecular weight of approximately 100 kD. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase 8, β-catenin, presenilin 1, casein kinase II , as well as other extracellular proteins including the EGF receptor. CD324 is phosphorylated on multiple residues (S857, S866, S870, S872), and can be proteolytically cleaved at reside D769 by caspase-3. The 67A4 antibody has been shown to be useful for flow cytometry.
: n% t" y2 C0 S6 G4 X3 ICD29: CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMß1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.
7 o/ P% f. G1 b* a! N* ECD24: CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.* T9 b' C9 ]* a9 P4 R" x f. p6 h: d; H
CD9: CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins.
$ `5 R- E, L& O$ N- QStat3: STAT 3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to cytokine receptor-associated kinase activity. Phosphorylation of STAT 3 induces nuclear translocation to activate transcription. STAT3 is expressed in brain, heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. STAT 3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT 3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT 3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT 3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by Rac1 protein. STAT 3 interacts with Rac1, STAT 1, and NCOA1 proteins. The Poly6246 antibody recognizes the C-terminal region of human, mouse, and rat STAT3 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry. The Poly6246 antibody does not react with STAT3b.7 `" T; P& x8 M8 c6 z/ g& ?: Q+ F% h
Nanog: Nanog (Homeo box transcription factor nanog) is a transcription factor, express in embryonic stem (ES) and carcinoma (EC) cells. Nanog binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Nanog involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. The poly6320 antibody recognizes human Nanog and has been shown to be useful for Western Blotting.
1 N: s8 T" y6 s9 @Sox2: This rabbit polyclonal antibody recognizes human SOX2 also known as SRY (sex determining region Y)-box 2 and ANOP3. SOX2 is a nucleus protein, predicted molecular weight 34 kD. SOX2 belongs to the SOX (SRY-box containing gene) gene family which encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. Defects in SOX2 are a cause of true or primary anophthalmos, also called anophthalmia or ANOP3. Anophthalmos is a developmental defect characterized by complete absence of the eyes (rare) or by the presence of vestigial eyes. The poly6309 antibody has been shown to be useful for Western blotting.+ D& I T/ u3 g: E1 W" X# U! K: J* q- Z
FOXD3: The FOXD3 polyclonal antibody recognizes human FOXD3 also known as Forkhead box protein D3 and HNF3/FH transcription factor genesis. FOXD3 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain, predicted molecular weight 48 kD. FOXD3 binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. FOXD3 is essential for multiple developmental processes in the vertebrate embryo, including neural crest development and maintenance of mammalian stem cell lineages. The poly6317 antibody has been shown to be useful for Western blotting.2 x& X* ^3 O+ y) ^/ S5 i
Oct3/4: Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and a central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct-4 is downregulated thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is nucleus protein, with a predicted molecular weight 39 kDa. The poly6318 antibody recognizes human Oct4 and has been shown to be useful for immunofluorescent staining.
% l, l5 U% p2 W2 c6 nc-Myc: The c-myc protein is a 62 kD nuclear factor that is ubiquitously expressed in the nucleus. c-myc is part of a heterodimeric complex with MAX that acts as a potent transcriptional activator. c-myc is modified by glycosylation and phosphorylation and has been shown to interact with a number of proteins including SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, and Sp1. c-myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that the observed protein size is approximately 41 kD. The 9E10 monoclonal antibody recognizes human myc and the 10 amino acid epitope tag of human c-myc. The 9E10 antibody has been shown to be useful in a number of applications including Western blotting, direct ELISA, flow cytometry, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), and immunoaffinity purification of proteins expressing the human c-myc tag.
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发表于 2011-4-12 23:21
发表于 2011-4-14 21:41
