|
  
- 积分
- 0
- 威望
- 0
- 包包
- 483
|
作者：Agnieszka Stokowskia,b,c, Songtao Shid, Tao Sunc, Peter Mark Bartolde, Simon Andrea Koblara,b, Stan Gronthosc作者单位：aAustralian Research Council, Centre for Molecular Genetics of Development,bSchool of Molecular and Biomedical Science (Genetics), andeColgate Australian Clinical Dental Research Centre, Dental School, University of Adelaide, Adelaide, South Australia, Australia;cMesenchymal Stem Cell Group, Divisio ! h! A4 ~+ M% c1 }0 j
2 J) {. x8 e& O+ G6 b8 y7 j& k: `, O
5 q7 k/ L& N( R. ^
) X5 D0 {0 O7 N1 @( s2 x3 {
# Z. f# ]2 x8 t7 v+ o 4 |3 G$ y$ G: ~
8 h+ R+ r$ f" g9 S5 E5 o G; b 8 L: y0 u, m8 j' V/ e/ q
) G0 T6 \9 ]6 P, Q- p
& V% F5 G' S1 N' d1 q9 V 7 ?; n* w* T0 C2 v9 p7 K
8 _7 p" G! w0 W5 C- `( W
4 V( f, l p8 @* f# P
【摘要】
7 D. ^# v7 A3 d# A/ ?; W/ a! z E- R Human adult dental pulp stem cells (DPSCs) reside predominantly within the perivascular niche of dental pulp and are thought to originate from migrating neural crest cells during development. The Eph family of receptor tyrosine kinases and their ligands, the ephrin molecules, play an essential role in the migration of neural crest cells during development and stem cell niche maintenance. The present study examined the expression and function of the B-subclass Eph/ephrin molecules on DPSCs. Multiple receptors were primarily identified on DPSCs within the perivascular niche, whereas ephrin-B1 and ephrin-B3 were expressed by the surrounding pulp tissue. EphB/ephrin-B bidirectional signaling inhibited cell attachment and spreading, predominately via the mitogen-activated protein kinase (MAPK) pathway for forward signaling and phosphorylation of Src family tyrosine kinases via reverse ephrin-B signaling. DPSC migration was restricted through unidirectional ephrin-B1-activated EphB forward signaling, primarily signaling through the MAPK pathway. Furthermore, we observed that ephrin-B1 was downregulated in diseased adult teeth compared with paired uninjured controls. Collectively, these studies suggest that EphB/ephrin-B molecules play a role in restricting DPSC attachment and migration to maintain DPSCs within their stem cell niche under steady-state conditions. These results may have implications for dental pulp development and regeneration.
3 z: v7 f* ~; M' ?6 X 【关键词】 Eph Ephrin MSC Dental pulp stem cell Migration Adhesion Spreading/ M# x7 \$ T, K+ `9 t2 O
( [7 O$ U4 \5 j' v& F0 ?1 G! I
【参考文献】8 w/ x* F! k& P1 ^
& \0 y: N0 f( F& T% d$ D m5 V. I2 @$ Q, O r& b
Chai Y, Jiang X, Ito Y et al. Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis. Development 2000;127:1671¨C1679.; l2 O$ Z* h( v3 m: U
9 y) d& K% z4 b2 k0 g( y) I4 _2 @2 f3 B
Miura M, Gronthos S, Zhao M et al. SHED: Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A 2003;100:5807¨C5812.' ?6 r7 r! c B
: P! }4 Y- g! @: [9 e9 u( ]% s
Gronthos S, Mankani M, Brahim J et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A 2000;97:13625¨C13630.
, c* h+ b5 v3 }* U3 [5 `. J
( m+ ?4 H, m* HGronthos S, Brahim J, Li W et al. Stem cell properties of human dental pulp stem cells. J Dent Res 2002;81:531¨C535.
, C+ o( z& C* `8 x# ?" d) c+ Z; V) `# o) U. V7 y
Shi S, Gronthos S. Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res 2003;18:696¨C704.
3 k$ U: I! ]. {7 z1 O" Z; B
, ^- F8 \4 M5 C2 X* lSchor AM, Canfield AE. Ostergenic potential of vascular pericytes. In: Beresford J, Owen M, eds. Marrow Stromal Cell Culture. 1st ed Cambridge, U.K: Cambridge University Press,1998;.
% B# K% l" e) |, m; U% t B! L8 e
* Z7 P7 z4 r, U: w" lBatouli S, Miura M, Brahim J et al. Comparison of stem-cell-mediated osteogenesis and dentinogenesis. J Dent Res 2003;82:976¨C981.1 O2 ^) i* b g$ x* _& c( d0 _0 Y6 Y
1 A4 u5 [* {$ O" u) |Tecles O, Laurent P, Zygouritsas S et al. Activation of human dental pulp progenitor/stem cells in response to odontoblast injury. Arch Oral Biol 2005;50:103¨C108.
0 a6 f1 T, N$ U* W& o0 ~0 H% K( q1 E3 M+ p3 \
Fuchs E, Segre JA. Stem cells: A new lease on life. Cell 2000;100:143¨C155.
# \8 C1 [) k1 U% F6 s: d3 H( } t* O6 H8 e0 c: `
Conover JC, Doetsch F, Garcia-Verdugo JM et al. Disruption of Eph/ephrin signaling affects migration and proliferation in the adult subventricular zone. Nat Neurosci 2000;3:1091¨C1097.. K3 P; _" i8 }9 r
9 B6 Y' s4 ~- q$ j6 }' S
Holmberg J, Armulik A, Senti KA et al. Ephrin-A2 reverse signaling negatively regulates neural progenitor proliferation and neurogenesis. Genes Dev 2005;19:462¨C471.. t: D$ V/ }. Y
# p" m! N) Y6 u7 N
Luukko K, Loes S, Kvinnsland IH et al. Expression of ephrin-A ligands and EphA receptors in the developing mouse tooth and its supporting tissues. Cell Tissue Res 2005;319:143¨C152.4 Q& G/ k8 `2 i+ P, _% O' B7 R4 q
9 ^% l) }9 ?1 J" Y! ~2 O: B- z2 q+ mGale NW, Flenniken A, Compton DC et al. Elk-L3, a novel transmembrane ligand for the Eph family of receptor tyrosine kinases, expressed in embryonic floor plate, roof plate and hindbrain segments. O |
|
发表于 2009-3-5 01:00
发表于 2015-5-21 19:01
