一种用条件RNA干扰研究鼠基因功能的快速可扩展系统

mcherry.f

Dr. Scott Lowe is the leading scientist in RNAi mouse model establishment. The point they addressed most is how to construct such a model rapid and scalable. And the answer they gave is to use tetraploid complementation based way to construct transgenic mice directly, not the typical pro-nucleus injection. In this manner, they can construct the transgenic model directly from a genetic modified ESCs, which can be either manipulated originally or from other disease model, making multi-allelic manipulation achievable. For the application, besides its technical advantage, the most important thing is the gene studied can be loss-of-function reversible, then allow us to study its function in a specific window of development.