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I have performed NSC culture of P0-brain and E14 spinal cord whcih never need enzyme to dissociate the neural tissue. the mechinal dissociation worked well with 1ml tip by gentle blow around 50-60 T. 5 `1 A c7 s; Y) {% M
" }( z2 d1 ~3 H& a7 R
After several passage, the Neurospheres turned to be very tight then need trypsin for dissociation. |
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