滋养层细胞的缺陷导致由体细胞核移植得到的克隆胚胎在体内发育受阻

laputave

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5 m- Q' ~$ k. p  ?7 R% IDefects in Trophoblast Cell Lineage Account forthe Impaired In Vivo Development of Cloned EmbryosGenerated by Somatic Nuclear Transfer! x, W; Y0 b: d3 U
滋养层细胞的缺陷导致由体细胞核移植得到的克隆胚胎在体内发育受阻  f/ ?8 r* S, o' T5 G- @+ P
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源自：Cell Stem Cell  Brief report) m' ^1 l' V, ]  f6 z2 n3 r+ T1 \

* Y; R; B8 @: E( p. ~0 H注明：由干细胞之间新闻小组成员laputave翻译，转帖请注明
1 }4 Z& a2 `% l      个人理解，翻译不妥之处还请各位老师不吝赐教。。谢谢咯。。(*^__^*)
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# S# O( ]$ Y2 g/ ~: R# L5 M' lJiangwei Lin,1,2 Linyu Shi,1 Man Zhang,1 Hui Yang,1 Yiren Qin,1 Jun Zhang,2 Daoqing Gong,2 Xuan Zhang,1 Dangsheng Li,37 b) ~7 H' S, o( e. @2 S
and Jinsong Li1,*8 y! t! C9 a3 {' s
1Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,
7 o: R. t4 B, fChinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200031, China: J' U$ v: ?" j* A/ G
2College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China
2 t7 g2 i% f; ~; |4 c5 X+ n3Shanghai Information Center for Life Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, China  G1 e7 U8 L- B% D4 ~* g( J6 r
*Correspondence: jsli@sibs.ac.cn- P+ Y+ H" w% ~
1.中科院上海生命科学研究院生物化学与细胞生物学研究所，分子细胞生物学实验室，上海岳阳路320号，200031* O. x' X4 o4 }* ~8 x
2.扬州大学动物科学与技术学院，扬州，225009
* G: b" |  t& x, I, T2 M3 o3.中科院上海生命科学院，上海生命科学信息中心，200031，联系方式：jsli@sibs.ac.cn5 m3 |; \% B2 W, G

+ L& W% T) O- ~- H- f  cSUMMARY
7 Y4 G# i$ i0 {/ |: K) l+ m! w  HThe low success rate of somatic nuclear transfer (NT)is hypothesized to be mainly due to functional defects in the trophoblast cell lineage rather than
& ]* M' V# f" ]( Ythe inner cell mass (ICM); this hypothesis, however,remains to be tested directly. Here we separated the ICMs from cloned blastocysts and aggregated5 G, L0 B0 E; o  w
the cloned ICM with two fertilization-derived (FD)tetraploid (4N) embryos. We found that the full-term development of cloned ICMs was dramatically4 m1 a/ N4 I1 h4 w, z6 N
improved after the trophoblast cells in the cloned blastocysts were replaced by cells from tetraploid embryos, thus providing direct evidence that defects, Y, z' r- W% z, b! b. V
in trophoblast cell lineage underlie the low success rate of somatic NT.9 j& M9 b5 x$ W1 G5 C$ l) G

1 e; |% P7 v/ M- c4 r1 K3 D, ~. x摘要
. a" X2 f* o, o) ?2 u& J+ F# b    体细胞核移植的低成功率的原因假设为滋养层细胞功能上的缺陷，而不是内细胞团的问题。然而，这个假设需要进一步的准确检验。我们从体细胞克隆的囊胚期胚泡中分离内细胞团，并将这些内细胞团细胞嵌合入受精来源的4倍体胚胎中。我们发现完整发育的克隆内细胞团细胞的发育在滋养层细胞被4倍体胚胎细胞取代后出现了戏剧性的改善，因此这为潜在于体细胞核移植之下的滋养层细胞缺陷提供了直接证据。
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laputave

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laputave

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宗介

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细胞海洋

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宗介

多谢了