Brain research：华西医院实现活体干细胞分子成像

细胞海洋

近日，我校华西医院放射科临床磁共振研究中心、生物材料工程研究中心和中山大学的相关研究团队合作完成一项脊髓损伤动物模型的磁共振分子影像实验研究，结果发表在脑研究领域知名刊物《Brain Research》（2011, in press）。
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分子影像研究是影像学发展的一个热点方向，能够对疾病的分子病理过程实现可视化的活体追踪。在当前的活体磁共振分子影像研究中，大多采用氧化铁纳米颗粒作为分子探针，但动物血液红细胞中的内源铁离子会干扰氧化铁纳米颗粒的信号，对于出血性的病灶损伤效果不好。磁共振研究中心的本项研究使用了临床常规使用的造影剂Gd-DTPA标记的脊髓间质干细胞，而后移植入脊髓损伤的大鼠模型。通过一系列体外的细胞组化实验、纵向活体磁共振成像检查，以及荧光显微成像实验证实，造影剂对干细胞的标记具有很高的转染效率，并表现出很高的在体存活率，有益于大鼠脊髓损伤修复。重要的是，通过这一磁共振分子成像技术，实现了对这一过程的活体监测。
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这项实验的重要意义在于，使用临床磁共振成像仪和造影剂实现了对小动物的分子成像，为今后人类神经与精神疾病的磁共振分子成像研究及临床应用奠定了基础。（生物谷Bioon.com）
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! _: [/ {: S* G生物谷推荐原文出处：
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Brain research   DOI: 10.1016/j.brainres.2011.03.032
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; m0 m2 ?) O; k0 e% MEvaluation of cell tracking effects for transplanted mesenchymal stem cells with jetPEI/Gd-DTPA complexes in animal odels of hemorrhagic spinal cord injury.7 f5 z; ~, J% j
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Yu Liu, Zhi-Jie He, Bo Xu, Qi-Zhu Wu, Gang Liu, Su Lui, Qian Zhong, David Deng, Hua Ai, Qiang Yue, Yi Wei, Shen Jun, Guangqian Zhou and Qi-Yong Gong' W4 X: T1 r( \  _) r0 n
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Cell tracking using iron oxide nanoparticles has been well established in MRI. However, in experimental rat models, the intrinsic iron signal derived from erythrocytes masks the labeled cells. The research evaluated a clinically applied Gd-DTPA for T1-weighted positive enhancement for cell tracking in spinal cord injury (SCI) rat models. MSCs were labeled with jetPEI/Gd-DTPA particles to evaluate the transfection efficiency by MRI in vitro. Differentiation assays were carried out to evaluate the differentiation ability of Gd-DTPA-labeled MSCs. The Gd-DTPA-labeled MSCs were transplanted to rat SCI model and monitored by MRI in vivo. Fluorescence images were taken to confirm the MRI results. Behavior test was assessed with Basso, Beattie, and Bresnahan (BBB) scoring in 6weeks after cell transplantation. The Gd-labeled MSCs showed a significant increase in signal intensity in T1-weighted images. After local transplantation, Gd-DTPA-labeled MSCs could be detected in SCI rat models by the persistent T1-weighted positive enhancement from 3 to 14days. Under electronic microscope, Gd-DTPA/jetPEI complexes were mostly observed in cytoplasm. Fluorescence microscopy examination showed that the Gd-labeled MSCs survived and distributed within the injured spinal cord until 2weeks. The Gd-labeled MSCs was identified and tracked with MRI by cross and sagittal sections. The BBB scores of the rats with labeled MSCs transplantation were significantly higher than those of control rats. Our results demonstrated Gd-DTPA is appropriate for cell tracking in rat model of SCI, indicating that an efficient and nontoxic label method with Gd-DTPA could properly track MSCs in hemorrhage animal models.

saberly

这个工作不错哈，我们也能做很棒的Image！！

yangwy028

好消息啊