干细胞之家 - 中国干细胞行业门户第一站

 

 

搜索
干细胞之家 - 中国干细胞行业门户第一站 干细胞之家论坛 干细胞文献资源库 文献互助区 求助一篇文献:nucleosome organization reconstituted
朗日生物

免疫细胞治疗专区

欢迎关注干细胞微信公众号

  
查看: 7778|回复: 0
go

[已解决求助] 求助一篇文献:nucleosome organization reconstituted [复制链接]

Rank: 2

积分
74 
威望
74  
包包
458  
楼主
发表于 2011-5-30 16:35 |显示全部帖子 |倒序浏览 |打印
Science. 2011 May 20;332(6032):977-80." i& I0 Z8 i* o/ b3 I) s" w
A packing mechanism for nucleosome organization reconstituted across a eukaryotic genome.1 i/ q* Z' E( c  S
Zhang Z, Wippo CJ, Wal M, Ward E, Korber P, Pugh BF.0 [3 U& Q) v& c- ?% O# c+ C
SourceCenter for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
+ e8 e. C* z+ d/ P- `: e; \; w, J" P) k9 d. g4 |
Abstract/ R2 \. _, P( K
Near the 5' end of most eukaryotic genes, nucleosomes form highly regular arrays that begin at canonical distances from the transcriptional start site. Determinants of this and other aspects of genomic nucleosome organization have been ascribed to statistical positioning, intrinsically DNA-encoded positioning, or some aspect of transcription initiation. Here, we provide evidence for a different explanation. Biochemical reconstitution of proper nucleosome positioning, spacing, and occupancy levels was achieved across the 5' ends of most yeast genes by adenosine triphosphate-dependent trans-acting factors. These transcription-independent activities override DNA-intrinsic positioning and maintain uniform spacing at the 5' ends of genes even at low nucleosome densities. Thus, an active, nonstatistical nucleosome packing mechanism creates chromatin organizing centers at the 5' ends of genes where important regulatory elements reside.
5 ^" J( K/ j9 h$ m6 M6 o6 i3 E- k% o7 b' W/ ^
‹ 上一主题|下一主题
你需要登录后才可以回帖 登录 | 注册
验证问答 换一个

Archiver|干细胞之家 ( 吉ICP备2021004615号-3 )

GMT+8, 2025-6-11 05:20

Powered by Discuz! X1.5

© 2001-2010 Comsenz Inc.