CpG: 完全重叠 – 阻止酶切 【什么意思啊？】

wnavy200299

如题：CpG: 完全重叠 – 阻止酶切    什么意思啊？7 p$ d: j, C: i- q" E
          什么叫：CpG: 完全重叠 ？
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2011-6-14 20:55 上传

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就是这个酶  ：http://www.fermentas.com/cn/prod ... -enzymes/er062-pvui

mcherry.f

For specific restriction enzymes, as PuvI you mentioned here, are sensitive to DNA methylation. For eukaryotes and prokaryotes, there are different DNA methylation system and result in different DNA methylation style. If you want to use PvuI, the concerns are:0 Q2 W6 ?% \; l; N& q
(1) Dam insensitive: the PvuI site in your plasmid, even stocked in Dam+ E.coli can be digested entirely.
0 H* B; s+ B7 w0 u& z* Z5 d(2) CpG sensitive: the PvuI site in eukaryotic genomic DNA cannot be cut by PvuI due to the CpG methylation (except CpG islands)." ^$ D8 Z4 T0 ~
For details, I suggest you to read the technical reference provided by NEB. & M5 Z# M, m: y/ I0 G0 d: m
URL: http://www.neb.com/nebecomm/tech ... cpg_methylation.asp

wnavy200299

回复 mcherry.f 的帖子
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eukaryotic genomic DNA cannot be cut 。2 X* |: F% A+ Z( v/ H
就是说在真核中，DNA被甲基化，不能被切开。- H9 D% ~  e3 c4 X
我是在大肠杆菌中扩增质粒后，进行酶切，应该没事吧？
  y4 Y. k/ [) W4 K, t谢谢~~~

mcherry.f

回复 wnavy200299 的帖子" {- g& _+ R2 m8 ?. M: K6 d

) c$ e# T; w: T; F- @) EYeah, this enzyme is not sensitive to the Dam system in E.coli.