人的脂肪干细胞好养吗

SUSHI妈妈

上了一年的博士,结果怀孕生孩子,实验什么都没弄,老板说做脂肪干细胞,结果被同学打击说最好不要碰干细胞,不知道都分化成什么了,请问大家人的脂肪干细胞好养吗

大少爷

脂肪细胞培养
' `- O8 c6 _/ Z; r) s! [- J3 b　　将吸脂来源的脂肪组织用D-Hanks 冲洗，去除残留的血细胞和组织碎片，把脂肪组织放入离心管中，记录原始体积。向组织中加入等体积的0.1%Ⅰ型胶原酶，放入37℃气浴振荡器中消化30 min。然后将消化好的组织静置5 min，待其分层后，用吸管吸取位于悬液上层的脂肪细胞，将含有脂肪细胞的悬液以1000 r/min(167g)，离心2 min 后弃掉下层悬液得到脂肪细胞。 ) G+ j7 ^* \% W
脂肪干细胞的分离和培养
2 L! B1 i  r! g    同上述脂肪细胞的分离一样，将脂肪组织冲净，称重后消化。当消化完成后，弃掉上层未消化的脂肪组织，将下层的细胞悬液以1500 r/min(375g)，离心10 min 后弃上清，得到离心管底部的脂肪干细胞团，将其重悬，密度调整到1×105mL-1，然后接种在培养面积为25 cm2 的细胞培养瓶中。 当干细胞长满培养瓶后按一传二进行传代，具体的传代方法是：首先，用D-Hanks 冲洗一遍细胞，然后加入2 mL 的混合酶溶液(0.25%胰酶和0.02%EDTA)消化，当在显微镜下观察到细胞发生明显的变形，缩成球状后，立即用含有10%胎牛血清的培养基中止消化，并用吸管轻轻吹打瓶底部，确保细胞完全脱落分离到悬液中，再把细胞悬液置于离心机上，以1000 r/min (167g)，离心5 min。最后，将离心得到的细胞团重悬，调整密度为合适密度进行接种。

jqm12345

不好养，最好不要养，很麻烦的

beginner

养了半年，感觉干性不是很好维持。其他目前没体会。

ygplay

本条信息也许对你有参考！' l% }: R, O/ R- w
HOUSTON - (June 16, 2011) - Innovative adult stem cell research by scientists at The University of Texas Health Science Center at Houston (UTHealth) could aid efforts to apply the brakes to stem cells that produce the type of fat ringing the waists of millions. Research appeared today online ahead of the print issue of Cell Stem Cell.
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) X+ I; @- T+ r" t/ dThe scientists have developed a bold approach for targeting fat-generating stem cells that one day could aid in the delivery of drugs that slow the cells’ ability to direct fat expansion. Because these cells are also used in regenerative medicine, this approach may have wide applications. The cells are termed adipose stem cells.
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The researchers used small artificial proteins (peptides) in a mouse model to identify a marker on the surface of adipose stem cells. Markers are molecules specifically expressed on individual cell types. The scientists screened about 100 billion peptides before finding one that was specific for mouse and human adipose stem cells.
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$ J5 b8 b5 v* U1 L; ]9 K4 z“This marker, called delta-decorin, is specifically expressed on the surface of adipose stem cells, which are responsible for the production of white adipose tissue,” said Mikhail Kolonin, Ph.D., the study’s senior author and assistant professor of molecular medicine at the UTHealth Medical School. “This is the first prospective marker to be discovered for this particular type of adult stem cell.”
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9 Q5 T; i. n# |' _% ^9 M7 Q% aAlexes Daquinag, Ph.D., the study’s first author and postdoctoral fellow at the UTHealth Medical School, said, “In obesity, you have an overgrowth of white adipose tissue. It is made of adipocytes (fat cells) and one way to stop obesity is to target the progenitor cells – adipose stem cells.”  h- P" _8 I2 `2 |- \% F( m4 U
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Kolonin added, “The existing approaches to identify adipose stem cells are based on combinations of semi-specific markers and are unreliable. Now we are able to target these cells with a new peptide probe for the purpose of tracing them in the body or controlled elimination.”
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The researchers report that delta-decorin, a modification of a previously defined protein, interacts with another clinically important protein called resistin on the surface of adipose stem cells. “The decorin-resistin connection is particularly interesting because both decorin and resistin have been previously implicated in Type-2 diabetes and other inflammation-related disease,” Kolonin said.
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" {7 }' I! F- F“The next step will involve preclinical tests to see if we can develop the identified peptide into compounds that target these adipose stem cells,” Kolonin said. “By depleting the adipose tissue progenitors, we may be able to provide long-term control of white fat.”
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* M. ]# J- z% _9 J7 C0 EKolonin believes this approach could complement an anti-obesity compound he developed at The University of Texas MD Anderson Cancer Center before joining the UTHealth faculty. The compound called “Adipotide” works by targeting the blood vessels in fat and is licensed to Ablaris Therapeutics, Inc.& o9 s2 s7 v) X' g; z
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“Helping people lose weight might also reduce their risk of cardiovascular diseases, certain types of cancer, Type 2 diabetes and other obesity-related diseases,” said Kolonin. Previous studies in Kolonin’s laboratory have demonstrated the role of adipose stem cells in cancer progression.0 X6 _, k( O. ~7 h$ }" @8 L) \

& l; k$ d) T% Y* ~, U3 q) E4 [Other collaborators from the UTHealth Medical School include Paul Simmons, Ph.D., C. Harold and Lorine G. Wallace Distinguished University Chair and director of the Centre for Stem Cell Research; Yan Zhang, M.D., Ph.D., research scientist; and Felipe Amaya-Manzanares, research associate.
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. \/ h6 R+ {5 M, I% VThe study is titled “An Isoform of Decorin is a Resistin Receptor on the Surface of Adipose Progenitor Cells.” The work was supported by Komen for the Cure, the American Heart Association and the Cancer Prevention & Research Institute of Texas.' @* ^) w2 n% i9 f
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Kolonin’s laboratory is in the university’s Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases. He is on the faculty of the UTHealth Graduate School of Biomedical Sciences at Houston and is a John S. Dunn Research Scholar.
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天枰2012

不知道，你的实验结束了没有，结果怎样？我也再养，很不稳定，出来的话可以请教哈，呵呵！

sushimama

刚做实验没多久,现在细胞养到二\三代就都冻存起来了,为了方便下面的诱导分化,现在正在筛选药物浓度,qq13452114,经常联系

我的干细胞

我的人脂肪干细胞养了很长时间了，我觉得还挺稳定的，状态也不错。大家多交流