试着提了两次大鼠原代BMSC，均以失败告终，前辈们给些建议吧，万分感谢

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MSC比较难养，易老化，大鼠的更难。MSC应该是单层梭状，圆形贴壁的细胞应该是造血干。你可以在培养基中加点L-谷氨酰胺，15%FBS试试

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6 ?8 O$ n5 \. V& }细胞需要谷氨酰胺合成核酸和蛋白质，谷氨酰胺缺乏要导致细胞生长不良甚至死亡

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回复 ivantanqi 的帖子
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4 }# x% F8 i0 I$ @Contamination  P- L; M' {2 A2 n# H% q/ N
Hematopoietic lineage cells can contaminate mMSC culture. mMSCs isolated by plastic adherence are heterogeneous and contaminated by hematopoietic cells5 due to the fact that the fraction of hematopoietic cells is higher in both initial and passage cultures of murine marrow cells. mMSCs support the growth and proliferation of hematopoietic cells by secreting growth factors, such as interlukin (IL)-6, IL-7, IL-8, IL-11, IL-12, IL-14 and IL-15, macrophage-CSF, LIF, FLt-3 ligand and stem cell factor. This problem was solved using frequent medium change that may prevent adherence of many of the non-MSCs and hematopoietic cells to the culture dishes.