表达人PD-L1的porcine 与人异种器官移植

hyde

大学终于毕业了，一大堆破事……回归呀（不求加分，但求补缺）
5 D9 C" l9 U$ `  w  ^) UXenotransplantation May Be A Step Closer As A Result Of Genetic Alterations In Pig Tissue
/ ?6 S$ a3 Z& w- ~+ h' X原文来自于http://www.medicalnewstoday.com/releases/230228.php
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【有点标题党的感觉】
( U! |; Z5 V: J- KA genetic discovery by Chinese scientists may one day allow pig tissue to be transplanted successfully into humans. Their research presented in the Journal of Leukocyte Biology represents a major step forward toward filling the shortage of vital organs for human transplantation. At the core of their work, they showed that altering or overexpressing the human programmed death ligand-1 (PD-L1) molecule in the endothelial cells of pig arteries reduces the conditions that lead to rejection. This strongly suggests that humans could receive altered porcine organs with fewer complications.
+ e# k) y) ^0 e& p4 O& X( o中国研究者发现让猪血管内皮细胞过表达（为什么要altering，表示不知）人程序性死亡配体 (PD-L1)可以减弱异种移植时产生的排除反应……一大堆对于人器官移植的意义（鼓掌……）。
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"Genetically engineered pigs may someday overcome the severe donor organ shortage, and save human lives," said Qing Ding, Ph.D., co-study author from the Shanghai Institute of Immunology at the Shanghai Jiaotong University School of Medicine in Shanghai, China. 1 F, p$ _6 x( t6 {) j3 y

, K3 K# z3 e: NTo make the discovery, scientists conducted experiments using two groups of pig vascular endothelial cells. The first group was genetically engineered to express human PD-L1, while the second group was normal. When both sets of cells were exposed to human lymphocytes, lower rejection response occurred in the group with the altered gene, while higher rejection responses were seen in the normal cells. Study results suggest that human PD-L1 could be used as a novel therapeutic agent to enhance tolerance of xenotransplants and also supports the possibility of using human PD-L1 transgenic pigs as xenotransplant donors. Using this type of genetic engineering technique could potentially overcome current challenges related to successful pig/human transplant rejection. , m  D0 o+ N% O# ]+ l. Y
【好像是体外实验……】猪血管内皮细胞分为两组，移植进行基因改造，另一组是不处理的对照。将这些细胞与人淋巴接触，发现实验组的排斥反应较弱……. G  w1 G: A. M7 r2 y- `' m% ^
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"Xenotransplantation has the potential to fill a huge gap between the number of available human donor tissue and number of needy recipients," said John Wherry, Ph.D., Deputy Editor of the Journal of Leukocyte Biology, "but our understanding of the pathways that might enhance the acceptance and physiological function of organs from animals such as pigs remains incomplete. The study by Dr. Ding and his colleagues is a very substantial step forward in defining a key immunoregulatory pathway that could be targeted in this setting." ! {& q  i+ k: F; }, t; K* P8 ~& P
【一堆老生常谈的意义……】
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Qing Ding, Liming Lu, Xiaorong Zhou, Yun Zhou, and Kuang-Yen Chou. Human PD-L1-overexpressing porcine vascular endothelial cells induce functionally suppressive human CD4+CD25hiFoxp3+ Treg cells . J Leukoc Biol July 2011 90:77-86; doi:10.1189/jlb.1210691. 7 V( e, S' b9 x( K* V  ]5 _  N7 x

2 M1 |0 j' |4 i- G4 B表示还需要瞟一眼原文
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旅行爱

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