基因修饰iPS细胞可望成为“癌战T细胞军火库”（附原文）

sunsong7

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Stem Cells, Potential Source of Cancer-Fighting T Cells
) e5 R- C0 |8 P  NScienceDaily (Sep. 21, 2011) — Adult stem cells from mice converted to antigen-specific T cells -- the immune cells that fight cancer tumor cells -- show promise in cancer immunotherapy and may lead to a simpler, more efficient way to use the body's immune system to fight cancer, according to Penn State College of Medicine researchers.5 E3 c: V: m% W. D
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9 w% ?: Y( y7 ~4 T5 W"Cancer immunotherapy is a promising method to treat cancer patients," said Jianxsun Song, Ph.D., assistant professor, microbiology and immunology. "Tumors grow because patients lack the kind of antigen-specific T cells needed to kill the cancer. An approach called adoptive T cell immunotherapy generates the T cells outside the body, which are then used inside the body to target cancer cells."* S) a1 k5 G& Z0 B3 _

% I1 l' P3 [3 G( U3 A8 w6 j- _It is complex and expensive to expand T cell lines in the lab, so researchers have been searching for ways to simplify the process. Song and his team found a way to use induced pluripotent stem (iPS) cells, which are adult cells that are genetically changed to be stem cells." S8 K, G/ C, C+ a/ n

% D- m, D3 z4 L3 e4 H  A8 J4 `: c"Any cell can become a stem cell," Song explained. "It's a very good approach to generating the antigen-specific T cells and creates an unlimited source of cells for adoptive immunotherapy."; X3 r+ D0 v; h

& w4 I2 T. Q% a9 A: {By inserting DNA, researchers change the mouse iPS cells into immune cells and inject them into mice with tumors. After 50 days, 100 percent of the mice in the study were still alive, compared to 55 percent of control mice, which received tumor-reactive immune cells isolated from donors.
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Researchers reported their results and were featured as the cover story in a recent issue of the journal Cancer Research.
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A limitation of this potential therapy is that it currently takes at least six weeks for the iPS cells to develop into T cells in the body. In addition, potential side effects need to be considered. iPS cells may develop into other harmful cells in the body.# {  r9 d- \, c; q/ Q
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Researchers are now studying how to use the process in human cells.$ [$ _& @0 p/ `# [
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Other researchers on this paper are Fengyang Lei, and Rizwanul Haque, Department of Microbiology and Immunology; Lynn Budgeon and Neil D. Christensen, Ph.D., Department of Pathology, Penn State College of Medicine.
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This study was funded through the Pennsylvania Department of Health using Tobacco Settlement Funds, the W.W. Smith Charitable Trust and the Melanoma Research Foundation.$ _8 N1 r' e& I) I

7 S6 x' `8 a5 o( d" Shttp://www.sciencedaily.com/releases/2011/09/110920111812.htm
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3 }: l& S( {0 c  P6 C# R4楼原文 感谢aidiulgy9517 提供

bigsnow55

很有意思！

naturalkillerce

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7 y1 R+ S5 P. m% @) t好新闻啊，总结概括如下：2 n: \. ~2 j$ E$ v
治疗癌症需要抗原特异性T细胞，但是癌症病人缺乏这些细胞，导致癌细胞不断生长。可以采用获得性T细胞免疫治疗，即在体外培养产生特异性T细胞，然后移入病人体内。但这种方法复杂而又昂贵。基于此，研究人员重编程小鼠体细胞得到的诱导性多功能干细胞(iPSCs)，然后在iPSCs中导入DNA片段，让iPSCs分化为抗原特异性T细胞，然后利用这些T细胞治疗患有癌症的小鼠，效果显著。当然还需评估可能的副作用，也需在人身上进行类似的免疫治疗验证。

aidiulgy9517

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& A) l& x6 \; q( i' w7 W$ y附上原文In vivo programming of tumor antigen-specific T lymphocytes from pluripotent stem cells to promote cancer immunosurveillance。2 }$ O9 n- o; G0 x1 X5 {/ K
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