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本帖最后由 细胞海洋 于 2011-10-8 20:37 编辑
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A guide to commonly used techniques for protein interaction analysis. * s: f/ w; s( W
Designed to help you determinine which technique or combination of techniques is best suited for your needs.% I6 _& }8 Y3 q& J6 T M
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Guide Contents; K( a2 |' p2 v; G; G0 Z' o
0 r' b0 z1 H9 t5 KYeast Two-hybrid System
3 b- y1 {5 X5 c6 |1 uMammalian Two-hybrid System' z; a0 w; q0 T" N W
Pull-down Assays' @( O! u+ k' b2 T# j0 S% J( B
Co-immunoprecipitation
: {+ b! p" A' C, eCo-localization/FRET/BRET
/ J3 ~5 [, X% x4 N$ I$ Z' h% y& YChromatin Immunoprecipitation& f/ G( T8 B6 ^4 ^4 n6 P. J, ]3 d; D
Electrophoretic Mobility Shift Assay7 |/ P$ e2 W% y f, K7 [4 K4 i
Appendix5 A& F- F P0 [4 r" [- V4 m5 ?
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发表于 2011-10-8 14:56
