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| Two-Phase Analysis of Molecular Pathways Underlying iPS Cell Induction. 参考中文标题： 点击查看机器翻译 作 者： Lin Z. Perez P. Lei D. Xu J. Gao X. Bao J. 全文及相关链接：John Wiley & Sons, Inc. Swets Information Services 摘 要： Induced pluripotent stem (iPS) cells can be reprogrammed from adult somatic cells by transduction with Oct4, Sox2, Klf4, and c-Myc, but the molecular cascades initiated by these factors remain poorly understood. Impeding their elucidation is the stochastic nature of the iPS induction process, which results in heterogeneous cell populations. Here we have synchronized the reprogramming process by a two-phase induction: an initial stable intermediate phase following transduction with Oct4, Klf4, and c-Myc, and a final iPS cell phase following overexpression of Sox2. This approach has enabled us to examine temporal gene expression profiles, permitting the identification of Sox2 downstream genes critical for induction. Furthermore, we have validated the feasibility of our new approach by employing it to confirm that downregulation of TGF-[gerds] signaling by Sox2 proves essential to the reprogramming process. Thus, we present a novel means for dissecting the details underlying the induction of iPS cells, an approach with significant utility in this arena and the potential for wide-ranging implications in the study of other reprogramming mechanisms. 参考中文摘要：诱导多功能干细胞(iPS)细胞可以被编程从成人体细胞与Oct4由转导,Sox2,Klf4,赵,但分子梯级由这些因素开始仍知之甚少。说明是妨害他们的随机性质的iPS诱导过程,结果在混合细胞群。这里我们有同步过程的重组两相诱导:最初的稳定的中间阶段,之后Oct4转导,Klf4,赵,和一份期末iPS细胞阶段特Sox2之后。这种方法已使我们的检查时间基因表达谱数据,通过健全,Sox2下游基因识别关键的感应。此外,我们已经验证新方法的可行性,我们利用这两种已经明确的确认TGF - - - - - -[gerds]信号Sox2必不可少的重组的证明过程。因此,提出了一种新的手段潜在的解剖细节iPS细胞的诱导方法,实用,在这个舞台上有显著的影响,而且可能广泛研究的其他重组的机制。出 处： Stem cells (Dayton, Ohio). 2011年 相关链接： PubMed Google学术搜索 Google引文 外部引文 相关文献 引文追踪(Google) |
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发表于 2011-10-16 23:16
