invitrogen的bFGF不会配啊~

20063585liu

我们实验室刚买进一管bFGF，说明书看不太明白，有谁用过，想请教一下怎么稀释和保存，谢谢啦~~附上说明书: Reconstitute in 100 μl of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/ml of bFGF.  To avoid loss due to adsorption, prepare dilute solutions in appropriate assay buffer containing at least 0.1% BSA just prior to use.  Do not store in dilute solution.  For longer term storage,  aliquot into buffer containing 0.1% BSA and store in polypropylene vials at -20°C. Avoid repeated freezing and thawing.  In applications requiring long term use of this growth factor in cell cultures, it is advisable to refilter material after dilution in BSA-containing buffer, through a 0.22 micron low protein-binding filter.