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invitrogen的bFGF不会配啊~ [复制链接]

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发表于 2011-11-2 09:05 |显示全部帖子 |倒序浏览 |打印
我们实验室刚买进一管bFGF,说明书看不太明白,有谁用过,想请教一下怎么稀释和保存,谢谢啦~~附上说明书: Reconstitute in 100 μl of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/ml of bFGF.  To avoid loss due to adsorption, prepare dilute solutions in appropriate assay buffer containing at least 0.1% BSA just prior to use.  Do not store in dilute solution.  For longer term storage,  aliquot into buffer containing 0.1% BSA and store in polypropylene vials at -20°C. Avoid repeated freezing and thawing.  In applications requiring long term use of this growth factor in cell cultures, it is advisable to refilter material after dilution in BSA-containing buffer, through a 0.22 micron low protein-binding filter.
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