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| 1 Laminin promotes formation of cord-like structures by Sertoli cells in vitro. 参考中文标题： 点击查看机器翻译 作 者： Hadley MA. Weeks BS. Kleinman HK. Dym M. 全文及相关链接：Elsevier Science 摘 要： Basement membranes are thin extracellular matrices which contact epithelial cells and promote their adhesion, migration, differentiation, and morphogenesis. These matrices are composed of collagen IV, heparan sulfate proteoglycan, laminin, and entactin as well as other minor components. Sertoli cells, like most epithelial cells, are in contact at their basal surface with a basement membrane. When cultured within three-dimensional basement membrane gels (Matrigel), Sertoli cells reorganize into cords that resemble testicular seminiferous cords found in the in vivo differentiating testis. Anti-laminin and anti-entactin antisera inhibit this cord morphogenesis by Sertoli cells whereas antisera against type IV and type I collagen, heparan sulfate proteoglycan, fibronectin, and preimmune sera had no effect. The RGD (RGDS-NH2) sequence, found in the cell binding domain of the integrin family of cell adhesion molecules as well as in the A chain of laminin and in entactin, effectively inhibited Sertoli cell cord formation at a concentration of 1.0 mg/ml but was unable to prevent Sertoli cell attachment at concentrations as high as 2.0 mg/ml. A synthetic pentapeptide from a cell-binding domain of the B1 chain of laminin. YIGSR-NH2, inhibited cord formation at a concentration of 0.25 mg/ml, but Sertoli cells were still adherent to the basement membrane matrix. At concentrations greater than 0.50 mg/ml, Sertoli cells detached. Antiserum against the YIGSR-NH2-containing sequence was also effective in inhibiting cord formation by Sertoli cells. Ligand (YIGSR-NH2 peptide) blot analysis of Sertoli cell lysates revealed an interaction with a major band at 60 kDa and with minor bands at 39 and 127 kDa. Furthermore, in Western blot analysis the anti-67-kDa laminin-binding protein antibody recognized a 59- to 60-kDa protein in Sertoli cells. The data indicate that laminin is involved in both Sertoli cell attachment and migration during formation of histotypic cord structures by these cells in culture. Two separate laminin cell-binding domains appear to be involved in Sertoli cell cord morphogenesis in vitro and are likely to participate in the formation of seminiferous cords in vivo. 参考中文摘要：基底膜薄细胞外基质的上皮细胞,促进其接触粘连、迁移、分化、和形态。这些矩阵是由胶原蛋白4、肝素硫酸蛋白多糖、层粘连蛋白,entactin以及其他小部件。牛肾上腺质嗜铬细胞,像大多数上皮细胞,在接触他们的基底表面基底膜上。当基底膜内三维培养凝胶(Matrigel),牛肾上腺质嗜铬细胞重组成类似于睪丸seminiferous连线绳索发现体内睾丸分化。Anti-laminin和anti-entactin antisera抑制这绳子的antisera牛肾上腺质嗜铬细胞形态而对IV型与I型胶原蛋白、蛋白多糖肝素硫酸、纤粘连蛋白、血清preimmune没有效果。RGD序列(RGDS-NH2)的,存在于细胞内的整合素家族结合细胞粘附分子以及一系列层粘连蛋白和entactin,牛肾上腺质嗜铬细胞形成有效地抑制脊髓浓度为1.0毫克/毫升但是却不能阻止牛肾上腺质嗜铬细胞连接的浓度高达2.0毫克/毫升。一种合成pentapeptide从cell-binding链的领地B1层粘连蛋白。YIGSR-NH2;抑制线形成浓度为0.25毫克/毫升,但牛肾上腺质嗜铬细胞仍附着于基底膜矩阵。在浓度大于0.50毫克/毫升,牛肾上腺质嗜铬细胞分离的。对YIGSR-NH2-containing序列检测也有效地抑制牛肾上腺质嗜铬细胞形成的绳子。配体(YIGSR-NH2肽)牛肾上腺质嗜铬细胞lysates上分析了一个互动具有较大的乐队在小乐队和60 kDa kDa 39和127年。此外,在西方分析67 -kDa抗吸laminin-binding蛋白抗体59 - 60-kDa承认牛肾上腺质嗜铬细胞中的蛋白质。数据表明我们牛肾上腺质嗜铬细胞对于两者来说都是需要连线和迁移形成histotypic绳索在这些细胞结构的文化。两个独立的领域层粘连蛋白cell-binding似乎牛肾上腺质嗜铬细胞形态参与绳子体外和有可能参与seminiferous体内形成连线。 |
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发表于 2011-11-3 13:17
发表于 2011-11-4 09:35
