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Detecting interactions with membrane proteins using a membrane two-hybrid assay in yeast8 u9 [0 {; b+ {7 }, ^4 c
http://www.nature.com/nprot/journal/v5/n7/full/nprot.2010.83.html
. s# A6 A4 b  UProduction of polyclonal antibodies against protein antigens purified by electroelution from SDS-polyacrylamide gel
( _- \& j- W' ?3 p8 Q" {http://www.nature.com/protocolexchange/protocols/635
# V/ {. R/ @/ CCo-immunoprecipitation of human mitochondrial proteases AFG3L2 and paraplegin heterologously expressed in yeast cells
- q- k& ^3 q. khttp://www.nature.com/protocolexchange/protocols/632
* y. E7 M3 F7 \! h- S1 L2 TCo-immunoprecipitation of Platelet Factor 4 and RANTES from human platelets
$ X3 d6 u7 [2 n. M* \9 M" hhttp://www.nature.com/protocolexchange/protocols/535
% Z0 l9 s3 ~+ q' R+ ]/ l; ^Use of in vivo biotinylation to study protein–protein and protein–DNA interactions in mouse embryonic stem cells4 ~, z3 T: }+ O, q; o
http://www.nature.com/nprot/journal/v4/n4/full/nprot.2009.23.html
: X! ^# y; p7 A) oDetergent-resistant membrane isolation
2 w, ]0 M& I0 b; ^4 ]: A2 mhttp://www.nature.com/protocolexchange/protocols/5392 M- a- r+ Y/ |6 b
Analysis protein complexes by 1D-SDS-PAGE and tandem mass spectrometry
  d5 f* ?% \- zhttp://www.nature.com/protocolexchange/protocols/455

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5  Use of in vivo biotinylation to study protein–protein and protein–DNA interactions in mouse embryonic stem cells
; w; I/ Y+ R, p4 ]. f2 U

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