PDF电子书：Human Cell Culture Protocols 3e （2012） Humana press

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PDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press0 h7 H/ \0 {3 U0 `3 m9 }

6 d3 I8 @! z$ N( b主要内容如下：
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& D0 z2 I* h5 @$ `, j& t Contents
: A: D/ }5 _, R* uPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii3 `/ d4 R/ }3 f2 p4 v) B9 A
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi' _' N$ Q! H: L# Q
  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  18 T' @: Y1 Z& ^( E
Christina Philippeos, Robin D. Hughes, Anil Dhawan, % n; J1 o) o+ Z# N, m
and Ragai R. Mitry( C( s3 A4 L) C+ |4 i# h2 |/ J
  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate . d& a2 y7 C. h: b+ ~
into Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  15
% b, P- E: T9 ILing Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn
3 Y( R; I- h8 x  3  Isolation, Cultivation, and Application of Human Alveolar 1 K5 ?. {4 B, g
Epithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31
4 U/ ~( Z% v; ~, O3 ?Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, 3 t0 U+ l( U* |& n6 [2 B
Hanno Huwer, and Claus-Michael Lehr$ ~1 {% ]5 ~  X/ {* |
  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  43# @3 y' s- C, s. w" i/ {* K! X
Peyman Björklund and Per Hellman
8 L( ^' H, R: N8 J  5  Functional Analysis of Human Islets of Langerhans Maintained
6 @+ Y' _6 g! Cin Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  55+ m  d% _. C$ O( D
Shanta J. Persaud, Bo Liu, and Peter M. Jones
5 j/ |7 L8 x% P, \  6  Conversion of Non-endocrine Human Pancreatic Cells
3 G& e7 l/ G% `6 h; kto Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73
1 h6 J/ b9 M5 J) wMin Zhao and Guo Cai Huang
# ]1 _3 x& l; M4 I3 }( n/ `  7  Evaluation of Cytochrome P450 Activities in Human 7 E9 U4 |2 b2 ?
Hepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  876 \0 J% t; M+ P1 r) P/ i, Q
María José Gómez-Lechón, Agustín Lahoz, José V. Castell,
& l9 @+ n0 N/ tand María Teresa Donato
( c- {. ^& _6 ?8 y* V  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  99
% i7 w) D) S- X3 z0 E* KDaniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka,
9 h& O( W: r, j$ X! WPeter Büchler, Michael Schoenberg, Jörg Kleeff, 8 L: T" \6 t# A. F/ W6 c9 }
Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy,
1 e3 ~' H+ k( a* ^Jan Hengstler, and Andreas K. Nussler
7 R. ~" I- r& M  9  Preclinical Testing of Virotherapeutics for Primary and Secondary
1 C6 R: s% @" N' `Tumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  121
7 M, A8 T) r4 s2 W& B' \Martina Zimmermann, Timo Weiland, Michael Bitzer, & T# C! H9 K% D4 i
and Ulrich M. Lauer
( q6 i" q8 X1 ^" s% P10  Isolation and Functional Studies of Human Fetal Gastric 2 ]; r, R4 H+ |
Epithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137, G# m2 C, B! `# ~0 G
Pierre Chailler, Jean-François Beaulieu, and Daniel Ménard
8 i+ I, l1 E7 ?& P1 N, {11  Isolation, Characterization, and Culture of Normal Human 6 k/ u1 S( u/ ]( g" t7 D, f+ ^
Intestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  157
, C9 p' d( s5 [% _6 O8 d% _' `Jean-François Beaulieu and Daniel Ménard
2 J/ h7 F7 [, i: A/ r6 y) u  Px Contents
; t6 b/ D. Y: f! p7 ^' z12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells 0 ]* X2 _  m. R
and Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  175
; _) k; j# r' L! ^  t4 jClaire C. Sharpe and Mark E.C. Dockrell
4 O, q. h. [- T7 U13  Glomerular Epithelial and Mesangial Cell Culture   ^2 V8 b1 O2 |, s1 I0 N
and Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  187+ q+ d2 h* ]' |  G2 N1 O
Heather M. Wilson and Keith N. Stewart
  p' D5 k( u% c: _- M14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  203, q7 P9 |7 U6 e$ _
Kirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried' L4 }4 C3 L& t* {! V- ^
15  Primary Culture of Human Adipocyte Precursor Cells:
: F  _" T0 k* j" G, e& MExpansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  215$ F) v  |. w1 T9 `/ S* P
Thomas Skurk and Hans Hauner
* x7 b* ^! K8 U1 h" V16  Primary Culture of Ovarian Cells for Research on Cell Interactions 4 P0 P4 p+ o! w) l' E7 S& `
in the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  227
3 @, `* V; {! Z% F$ Q- \* \Jerzy F. Galas2 s/ A  y" F3 @# r4 G+ f8 j
17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2516 g, n) P. I, x5 a
Diane Proudfoot and Catherine Shanahan
! X6 k1 D9 j+ h1 {- ]0 x18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  265
( h3 e" ]4 _& ^2 j2 ?Richard C.M. Siow
  C) X$ ~# R5 P% i. x8 e19  Human Peripheral Blood Mononuclear Cell Culture for Flow # D4 Z; p: `5 Z7 c
Cytometric Analysis of Phosphorylated Mitogen-Activated
% ?2 K- K* t7 e, n( x5 dProtein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  275
5 `  g- I$ e6 M% }' Y( IAthanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, * u2 U  j+ ~6 |; S% X0 q+ x
and Dimitrios P. Bogdanos
( ?, @$ @8 A3 ]1 ^20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  2876 j! r7 Z  M  J% ~! ]/ R
Haibin Su, Maria Serena Longhi, Pengyun Wang, / Y5 {: _0 I5 a8 ]
Diego Vergani, and Yun Ma( y* s3 ^1 U0 P: n
21  Human Chondrocyte Cultures as Models of Cartilage-Specific . x! K5 n4 Y4 ~
Gene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  301
7 H9 i8 J. ~! KMiguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, ) L% A0 o5 f' _/ ^) `7 L
Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring7 f7 H, |# l9 W
22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  337: o' H5 c* l$ ^! }* i4 l" M6 K
Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, 5 Y( |8 v; k  Z9 _1 ]% I+ _
and James A. Gallagher
+ ^6 }# R& J0 v# V23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  357
8 M( x  r9 Y/ T, H1 d, UAnkita Agrawal, James A. Gallagher, and Alison Gartland
: \: d! E4 ~5 q% }6 H24  Effects of Temperature Generated from the Holmium: YAG Laser ; ?5 J1 g, x' O% Z0 M2 Z3 O3 D3 `
on Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377
2 D8 ]* d& U" m$ u1 k' R/ xMoustafa I. Hafez, Anne Sandison, Richard R.H. Coombs,
8 e  N, k, ]+ G, h" \Ian D. McCarthy, and Al-Shymaa M. Hafez : ^1 v: s- [  n* N
25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  385
' c' D% b  N5 b. N* |0 }Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia5 A9 L9 D9 M, C: q0 A- ~# J3 a
26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  393
* c$ F" z4 a. N! a0 @5 M0 q2 nRobert Thomas and Elizabeth Ratcliffe
2 D$ p+ I! x3 E6 H5 r* n27  Culturing and Differentiating Human Mesenchymal Stem Cells . B% T6 r" q( C
for Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  407
/ R0 ^( b  i% x! q1 m) {: S' FWilliam R. Otto and Catherine E. Sarraf # a7 D; ?: L+ A- O% B0 R3 Z
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  427
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