临床干细胞分离方法讨论

yxc

1.主要依靠离心机的方法，才应该算是离心法
0 t" D1 Q4 {8 @6 g! q8 E; w) s, ]主要包括密度梯度离心法，包括Ficoll、peroll等分离液。, D: c7 q9 k/ Q9 h; F* V5 F  C; v$ Y
沉降法、负收集法是区别于密度梯度离心的特殊离心法，有各自的原理支持。& B6 \# A9 e% L( p  c  \
+ A5 g3 k" F: [& ]$ G, @
2.AXP Auto Xpress™ Platform系统是用来收集脐带血中的富含干细胞的部分，主要是为降低细胞采集期间受污染的几率提供一个密封及无菌的环境，与传统人工采集的原理并无本质区别，官网http://www.gelifesciences.com/ap ... t/LD_338528314-J4509 a. u5 b: o* u, r2 C# }
有pdf全文
1 o, V, ]4 J& _3 T. s  P
) E+ s) i. E; j8 U# P2 C3.对于多数刚开展干细胞研究的单位来说，密度梯度离心法是比较适合的，设备、试剂的获取、价位、可操作性比较理想，但对于计划今后大规模应用的单位，肯定有大厂家瞄准商机制造出更好的设备，期待权威分析。( I+ j, _. f) d4 U7 o3 d

yxc

ThermoGenesis AXP AutoXpress Platform

yxc

   关于负收集法和AXP自动系统两种方法所获取的干细胞的纯度，尚未见有人做出直接比较。4 ^2 i- c% }: y% [' M: l2 |
   AXP系统在国内主要应用于干细胞库建设，随着医院干细胞研究及临床应用的发展，将可能应用于外周血干细胞的采集，骨髓量小，一般不用吧。
3 L; e. ]& q; `' a$ g+ t) M   关于费用，希望是于成本呈正比的同时，尽量也与效果正相关。
8 S5 A' `  a/ j$ j; E1 V6 {- n   望指正。

yxc

回复 monday2019 的帖子; m6 y) S( E9 K8 v& [+ w, T
! \2 [7 E) z9 W8 C
   国内医院开展的干细胞治疗不仅有骨髓和外周血，还有脐带干细胞。
: O& J3 k" I; z5 d+ B3 B) G, f; C  你说的是MXP就是MarrowXpress™吧？没听说过国内的应用情况，估计还是受到骨髓的本身影响。* w3 m1 z2 T+ n7 }: Y

8 n3 t9 L8 i1 e1 A! b9 b2 m这是其介绍
3 ?: X1 e" @- a6 RAdvanced bone marrow processing, stem cell harvesting, and data tracking.
1 A/ J/ o. C1 P& @: n; gThe innovative MarrowXpress™ (MXP) System defines a new processing standard for isolating and retrieving stem cells from bone marrow aspirate. It’s an automated, fully closed, sterile system that volume-reduces bone marrow to a user-defined volume in 30 minutes, while retaining over 90% mononuclear cells (MNCs)**. Self-powered and microprocessor-controlled, the MarrowXpress platform contains flow control optical sensors which achieve precise separation.0 i  u( m) Q  j* p- {' U

; Y* m4 i5 P/ h9 I5 r' {1 M2 {, c7 h    Automated, closed system( r" K4 ~: k$ H% `' j
    Precise cell separation' T5 m0 e+ m" Y1 E  f
    Low residual red blood cell contamination
; ^0 ^: l' x7 U/ i' a8 Q0 _    Sterile in-line sampling
9 O  \% ]5 c$ z    Rapid sample processing
3 p# \$ r7 t6 g6 K9 h0 Z
, q" W/ f# j) }Hands-free operation — Automated, closed, sterile system.- `7 _+ E7 m# q5 B: \' M* G
4 e' `  V+ g4 V- B: ~4 R9 [' Q
High yields — Consistently high MNC recoveries and CD34+ recoveries.*
' D4 k4 e* g2 G( D; B
% a5 Y- m; |- T! kReliable volume reduction — Reproducible reduction of initial unit volume to a user-determined final volume.' k  [  {- o# [  ?  Q; P! p' y" Q, c
7 J: Z% m! s- R
Streamlines workflow — Simultaneous processing of up to six samples.
6 E) C% x) w+ B8 f$ z2 s1 v
! _$ P5 Z& N1 V% t1 g" RSafe operation —  In-line sterile sampling.
/ K4 D# M( z- C  Y+ u* ?# T( ?! U4 o$ _. l1 v
Easy data management — Quick and accurate data tracking with XpressTRAK™ software.4 @& r  q' M3 H% C$ V2 \' S

5 V5 E$ F4 T" ]- oRapid sample processing —  Sample separation and concentration complete in 30 minutes.2 ]7 ^. p8 R; ^# I6 Y) p
4 k  a$ w1 }2 O0 z5 j
Self-powered —  Powered by a rechargeable NiMH battery.; `1 ^4 x  Z  Q: ^& x$ V

4 b( H3 O9 A' C( Q8 o2 ]Real-time Communication — XpressLINK software provides the ability to link data from all AXP devices.2 r( Q$ Q# C7 u
Complete system solution — The MarrowXpress™ System consists of the docking station, processing set, and XpressTRAK™ software that assists with cGMP and cGTP compliance.' B4 d, t! b+ Y

yxc

干细胞实验及临床研究所包括的病种很广泛，包括用脐带干细胞，远远不只血液病。
, G, _% l. d& \1 ~2 \& j