|
- 积分
- 48
- 威望
- 48
- 包包
- 69
|
本帖最后由 细胞海洋 于 2012-1-13 14:38 编辑 5 I9 T5 R. o3 h; U# ^6 Q
! }3 \; ^8 I: W* U) c) A+ ]/ R1 m) q5 @4 p
Springer出版社的新书，很多图，对做人多能干细胞的童鞋可能有用。
8 s$ ~8 x0 r0 @% o( aISBN 978-1-61779-547-3 e-ISBN 978-1-61779-548-0
7 I! @- h! W& i+ e6 {DOI 10.1007/978-1-61779-548-0
/ h6 g# o( c7 R$ K7 E& gSpringer New York Dordrecht Heidelberg London' m, N9 g" R# @. i4 ~2 n$ q7 {, Q1 @( X
Library of Congress Control Number: 2011941608
1 a# s g9 U/ \, h9 J% T© Springer Science+Business Media, LLC 2012: \; U: F$ s, A/ h
[hide][/hide]
N8 X. d8 A5 M: R. N0 T: X( C4 nContents：
6 Q+ I9 Q1 I$ B# V1Methods for the Derivation of Human Embryonic
9 \$ O* C4 I" R) t0 \Stem Cell Lines .......................................................................................... 1
2 m/ }' S8 }/ i2 C8 `6 j1.1 Introduction ........................................................................................ 1
2 R7 q: u( G# n' U6 R1 W1.2 Materials for ESC Line Derivation .................................................... 9
8 p- Y$ |1 n* ?' o& |1.3 Methods for hESC Isolation .............................................................. 9
2 L6 `$ i9 J7 G1.3.1 hESC Isolation by Immunosurgery........................................ 125 i4 j% v s" e3 q: n
1.3.2 Mechanical Removal of Trophectoderm ................................ 12
G+ \% \8 \. I+ p2 L1.3.3 Whole Embryo Approach for ESC Line Derivation .............. 13
. c/ M a$ |# e2 Y$ _4 yReferences ................................................................................................... 133 s, o3 }8 z' }5 ]
2 Morphology of Human Embryonic and Induced Pluripotent: i7 t$ P$ Y T- F: d
Stem Cell Colonies Cultured with Feeders ............................................. 15
& l) K( T& |) d2.1 Introduction ........................................................................................ 159 M. C) v& H& ]) v
2.2 Materials ............................................................................................ 16
7 f& }3 t5 o( t' s/ `2.2.1 For Mouse Embryonic Fibroblasts (MEFs)# Y( u& G3 W/ {5 A' t9 P1 }
and Foreskin Fibroblasts (HFFs) ........................................... 168 Q% t' u. k5 Z9 ?2 D H4 z
2.2.2 For hPSC Maintenance .......................................................... 17
9 z, l4 R- Q: q6 [; ` N6 a! l2.3 Methods ............................................................................................. 18
; u: x$ Y! M2 \7 S2 ~' |( U$ k& P2.3.1 Feeder Culture Methods ........................................................ 188 R# M8 `, F0 ^: q; r" g8 `
2.3.2 hPSC Culture ......................................................................... 22
4 ^5 \4 q6 T# w3 k" B; Y1 I9 ~# SReferences ................................................................................................... 382 I) T! V9 @( S6 a" c; K% P% X
3 Morphology of Human Embryonic Stem Cells and Induced
, c6 d- L' z: c( J0 Q' K1 _Pluripotent Stem Cells Cultured in Feeder
' D% ], k* i& _Layer-Free Conditions .............................................................................. 41
) q, a1 c* Y3 D A2 G6 h3.1 Introduction ........................................................................................ 41/ ~/ k# S( I1 h( x- }
3.2 Materials for Feeder Layer-Free Culture of hPSCs ........................... 43
5 K% I1 d: ]4 f3.2.1 Matrix Preparation ................................................................. 43
# G3 U: S9 _/ o$ h- Q, W0 u3.2.2 Culture Medium ..................................................................... 449 G: T- I$ T y4 S
3.3 Methods for hPSC Feeder Layer-Free Culture .................................. 44
( u4 H, N2 a2 s6 X+ E+ o. D1 `" ]3.3.1 Preparation of Matrix-Covered Plates ................................... 44
/ `! x' T5 F3 F* B2 _& ~) o5 ~; m3.3.2 Splitting, Freezing, and Thawing hPSCs ............................... 45- s/ G% D" ], x: T& }
3.3.3 Adaptation of PSCs to Feeder-Free Culture .......................... 456 |( N0 s, V9 b6 G9 ]( ]$ s5 Y
3.3.4 Routine Culture of hPSCs ...................................................... 46# ^. ~1 f# S: W' @. T( F( B
References ................................................................................................... 54
6 l4 x' @, l S. ]$ A' i4 R4 Morphology of Undifferentiated Human Embryonic
* b9 e2 x% I+ F B& f$ s3 d# Sand Induced Stem Cells Grown in Suspension# E1 P0 {9 i# w4 {2 R" E( G
and in Dynamic Cultures .......................................................................... 57; N6 Q! V: O. k6 f5 Z5 Q0 r, K
4.1 Introduction ........................................................................................ 572 j* ?; [3 Y5 p& `4 }
4.2 Materials for Suspension Culture of hPSCs ...................................... 58% I! s! _7 z: _5 J, M; q5 ?
4.2.1 Culture Medium ..................................................................... 58# P1 I! N( r7 p4 q% o
4.2.2 Splitting Medium ................................................................... 59" Q" ?. Q8 O3 w- Y% H' n& t
4.2.3 Freezing Medium ................................................................... 59
- @7 X. `0 |- K' q9 [4 T$ E4.3 Methods for Suspension Culture of hPSCs ....................................... 60/ @' \1 u, p. m' d; a+ \# H6 N! H
4.3.1 Creating a hPSC Suspension Culture .................................... 60% \+ _: H( ^$ O7 p9 X* \
4.3.2 Splitting hPSCs in Suspension ............................................... 60
5 y3 A# l' D- J" J. S1 G4.3.3 Freezing hPSCs in Suspension .............................................. 624 |. _) E7 G; c2 o/ C
4.3.4 Thawing hPSCs in Suspension .............................................. 64
1 H/ f) O4 b- g2 s# g9 e1 W4 C0 N# g4.3.5 Culturing hPSCs in a Dynamic System ................................. 64: U- @2 U n$ w4 p) P/ h: R( V
4.3.6 Routine Culture of hPSCs in Suspension .............................. 657 F4 R2 d6 o) T+ n2 ?) d0 o
References ................................................................................................... 71; I# A' r- _+ Y; m ~
5 Differentiation of Pluripotent Stem Cells In Vitro:. B7 B% t0 ?) V" C, q
Embryoid Bodies ....................................................................................... 73' `; m/ y2 g0 a4 t; c! s% B0 e
5.1 Introduction ........................................................................................ 73, l8 C& z5 G" f" A8 F: C# ]. r b( w
5.2 Materials for EB Formation ............................................................... 75& _# y9 b7 d' A% c/ o* H
5.2.1 Culture Medium Supplemented with Serum ......................... 75
- w% w+ Y( y$ \; J |5.2.2 Splitting Medium Based on Collagenase ............................... 76
- z: ?% D# Q9 S+ l4 x+ \% |- }5.3 Methods for EB Formation and Culture ............................................ 76
; X/ t* }+ K: F; {9 C. n5.3.1 EB Formation ......................................................................... 76
D* I) l: j p5.3.2 Routine Culture of EBs .......................................................... 761 W! u; C# j7 w' G
5.3.3 Culturing EBs in Spinner Flasks ............................................ 78( t+ ?& R. n l7 D
References ................................................................................................... 88
: ?0 {# ^+ E. d: _2 L1 I& d6 Differentiation of Pluripotent Stem Cells In Vivo:) D% T$ D# u0 Q3 m8 a
Teratoma Formation ................................................................................. 918 w' \$ e2 r. Y D W' @
6.1 Introduction ........................................................................................ 91! s# Y9 U" w( ]* J4 O
6.2 Materials for Teratoma Formation ..................................................... 93+ ~" J; B# C; \& R7 y+ E, y, d
6.2.1 Culture Medium ..................................................................... 934 [! Z6 m/ ?8 M( c' M' Y4 x
6.2.2 Syringe for Injecting Cells ..................................................... 93
u b$ X9 E1 ^' |6 v6.3 Formation of Teratomas ..................................................................... 93
1 E% S- o$ @; {5 y6.3.1 Protocol for Teratoma Formation .......................................... 93
( O! k8 F7 f4 l' p ]$ \2 u6.3.2 Routine Treatment of Mice and Teratoma ............................. 93
7 W! O5 n6 q9 A9 O( iReferences ................................................................................................... 103
2 ^- |* e+ ?# p6 n: r% N& |) z) G7 Immunostaining ........................................................................................ 105
9 \9 }* ^2 K, \# z7.1 Introduction ........................................................................................ 105, \9 Q+ G% n9 i3 i( M
7.2 Materials and Solutions for Immunostaining .................................... 1113 c; ?+ q @# U+ {2 G4 v
7.2.1 Materials and Solutions for Immunohistochemistry# }4 o9 p! [2 H' m0 t
of Paraffi n-Embedded Tissues ............................................... 111% I. s9 ]% V- k. S
7.2.2 Materials and Solutions for Immunofl uorescence ................. 111
; w0 E1 N/ [; j, z6 [& n; T7.3 Immunostaining Procedures .............................................................. 112
# t- [' B; `, j3 p7.3.1 Immunohistochemistry of Paraffi n-Embedded Tissues ......... 112& h/ h6 K* l% D9 K
7.3.2 Immunofl uorescence of Cultured Cells ................................. 113
E5 u3 `. O. k# V1 k/ T" F4 xReferences ................................................................................................... 113
9 g* U1 k- O5 y k7 Z& o0 }8 Karyotype and Fluorescent In Situ Hybridization$ f8 D4 I9 G7 M4 O! @- a
Analysis of Human Embryonic Stem Cell and Induced
3 u- z% k! b8 G5 h# F- |Pluripotent Stem Cell Lines ..................................................................... 115+ k* H3 k: m o5 F! y; g4 @: g
8.1 Introduction ........................................................................................ 1154 u/ A/ S) V) t+ Q9 h5 @0 a
8.1.1 Karyotype Analysis ............................................................... 115
: [+ }+ T3 S V: D% F8.1.2 FISH Analysis ........................................................................ 121. t3 e" K+ s% m& H; U A1 A% ^
8.2 Materials for Harvesting Cells for Karyotyping( I6 b0 I# {8 e/ a
and FISH Analysis ............................................................................. 124
0 ?8 s6 i% \0 u6 F2 r( ?0 g& K8.2.1 Reagents ................................................................................. 1243 U/ H' E6 }- \: k
8.2.2 Solutions ................................................................................ 124: i" s' O F7 l% H& G$ w
8.3 Procedure of Harvesting Cells for Karyotyping
2 p6 g4 l( l+ aand FISH Analysis ............................................................................. 124) Q6 o8 b$ w# ~4 Y
References ................................................................................................... 1268 {) U Y1 U. l' f; A
9 Method for the Derivation of Induced Pluripotent
- Z( E2 F9 W' {) n2 YStem Cells from Human Hair Follicle Keratinocytes ............................ 127. c& U7 u6 M4 ?0 B
9.1 Introduction ........................................................................................ 1273 h- w$ d: H b6 U
9.2 Materials ............................................................................................ 129) L( l& w) ?; U' M7 h$ F7 K9 c( P P. Q
9.2.1 NIH-3T3/293T Cells .............................................................. 129
) E: J) `+ _( k9.2.2 Keratinocyte Derivation from Plucked Hair Follicles ........... 129
- Y! A: T+ K* R3 s1 @) W6 A7 `9.3 Methods ............................................................................................. 130+ M* P( Z7 G. u9 ~
9.3.1 NIH-3T3 and 293T Culture Methods .................................... 1302 @1 z# H9 k+ L1 U2 ]2 a8 }
9.3.2 Keratinocyte Culture Methods ............................................... 132% C G) J' a# f+ T9 L0 R% k& u' T
9.3.3 Preparation of the STEMCCA Virus for Infection ................ 133
* X; n& T6 t' y' d# A" z9.3.4 Derivation of iPSCs from Hair Keratinocytes ....................... 1341 |6 Q t8 j" J/ G4 V
References ................................................................................................... 137 |
附件: 你需要登录才可以下载或查看附件。没有帐号？注册
-
总评分: 威望 + 5
包包 + 20
查看全部评分
|
发表于 2012-1-13 13:55
