胶原酶、accutase还是dispase？

lchanlon

if you culture ips cells in feeder, the collagenase IV is a good choice. But for feeder free, Accutase and dispase is not good. I suggest you use 0.5 mM EDTA in PBS. for 33mm dish, add 2ml of 0.5 mM EDTA, incubate at room temperature for 3-5 minutes, aspirate the EDTA. Then add 1 ml of medium using  1 ml pipet, pipet up and down to disassociate the ips cell and transfer into a new tube.
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补充内容 (2012-9-22 10:12):
( }8 ~! ^) L0 [  fIn our lab, it works well. this protocol from James A Thomson laboratory