Disruption of cytoskeletal integrity impairs Gi-mediated signaling due - 《cell》细胞杂志干细胞之家

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发表于 2009-3-5 23:41 |只看该作者 |倒序浏览 |打印

Wilhelm Bloch1, Yun Fan2, Ji Han1, Sheng Xue2, Torsten Sch?neberg3, Guanju Ji2, Zhong J. Lu2, Michael Walther1, Reinhard Fssler4, Jürgen Hescheler2, Klaus Addicks1 and Bernd K. Fleischmann2

1 Institute of Anatomy I, University of Cologne, Germany2 m: l; r. l. m8 T9 h

2 Institute of Neurophysiology, University of Cologne, Germany& T+ Q- I8 N2 m4 D# j) G  C

3 Institute of Pharmacology, Free University of Berlin, Germany
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4 Department of Experimental Pathology, University of Lund, Sweden
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Address correspondence to B.K. Fleischmann, Institute of Neurophysiology, University of Cologne, Robert-Kochstr. 39, D-50931 Cologne, Germany. Tel.: 49-221-478-6940. Fax: 49-221-478-3834. E-mail: akp17@uni-koeln.de
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Abstract

?1 integrins play a crucial role as cytoskeletal anchorage proteins. In this study, the coupling of the cytoskeleton and intracellular signaling pathways was investigated in ?1 integrin deficient (-/-) embryonic stem cells. Muscarinic inhibition of the L-type Ca2  current (ICa) and activation of the acetylcholine-activated K  current (IK,ACh) was found to be absent in ?1 integrin-/- cardiomyocytes. Conversely, ? adrenoceptor-mediated modulation of ICa was unaffected by the absence of ?1 integrins. This defect in muscarinic signaling was due to defective G protein coupling. This was supported by deconvolution microscopy, which demonstrated that Gi exhibited an atypical subcellular distribution in the ?1 integrin-/- cardiomyocytes. A critical role of the cytoskeleton was further demonstrated using cytochalasin D, which displaced Gi and impaired muscarinic signaling. We conclude that cytoskeletal integrity is required for correct localization and function of Gi-associated signaling microdomains.
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Key Words: embryonic stem cells; signal transduction; ion channels; G proteins; 1 integrins6 J/ D( ^: J7 x7 Q6 d3 b5 `

Introduction, t: P8 Y2 F+ E% {' n0 _

Heart function, in particular the rhythm (chronotropy) and force of contraction (inotropy), is regulated by ?-adrenoceptors and muscarinic type 2 (M2)* receptor–mediated activation/inhibition of L-type Ca2  current (ICa) (chronotropy and inotropy) and acetylcholine-activated K  current (IK,ACh) (chronotropy) (Sakmann et al., 1983). The signaling cascade involved in the modulation of ICa has been described in detail (for review see Trautwein and Hescheler, 1990). In vivo (Redfern et al., 1999) and in vitro studies have suggested that pertussis toxin sensitive G proteins of the Gi family mediate muscarinic depression of ICa (Wickman and Clapham, 1995; Ye et al., 1999; Chen et al., 2001) and activation of IK,ACh (Sowell et al., 1997). The involvement of NO in M2 receptor–mediated depression of ICa in adult heart is controversial (Han et al., 1998; Vandecasteele et al., 1999). We previously demonstrated that the mechanism of muscarinic regulation of ICa switches during embryonic development from a NO-mediated depression of basal ICa to direct Gi-mediated lowering of adenylyl cyclase (AC) activity in late stage cardiomyocytes (Ji et al., 1999)., t8 C3 b; K( |) [& r
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Given that cytoskeleton-associated membrane receptors have been implicated in several heart disorders (for review see Towbin, 1998), we determined whether ?1 integrin receptors modulate ionic conductances critical for heart function. Since ?1 integrin-/- mice died shortly after implantation, chimeric mice or in vitro differentiated embryonic stem (ES) cell–derived ?1 integrin-/- cells (Fassler et al., 1996) were used. Previous studies revealed several abnormalities in ?1 integrin–deficient cells including migration and homing defects of haematopoietic cells (Hirsch et al., 1996), alterations in vasculo- and angiogenesis (Bloch et al., 1997), and severely disturbed cardiomyogenesis (Fassler et al., 1996). Although the biophysical characteristics of ionic conductances of ?1 integrin-/- ES cell–derived cardiomyocytes appeared normal, the action potentials generated by deficient cardiomyocytes exhibited a primitive phenotype at all stages of differentiation (Fassler et al., 1996). Furthermore, ?1 integrin-/- ES cell–derived cardiomyocytes exhibited abnormalities in cytoskeletal integrity. Herein, we investigated the role of ?1 integrins in hormonal regulation of ICa using ?1 integrin-/- cardiomyocytes.  e7 W' \: t5 \+ h  B  ]0 u+ O6 D

We report that ?1 integrin-/- cardiomyocytes lacked muscarinic signaling, whereas ?-adrenoceptor–mediated stimulation of ICa remained intact. This specific signaling defect was found to be related to spatial displacement of G proteins of the Gi-family.; g, U/ s# B) I3 L" Z; f2 P

Results
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Muscarinic depression of basal ICa was found to be absent in ?1 integrin-/- ES cell–derived cardiomyocytes

As previously reported (Ji et al., 1999), prominent inhibition (44.7 ± 8%; Fig. 1 d) of basal ICa density was observed upon application of the muscarinic agonist carbachol (CCh) (1 μM) (Fig. 1 a) in a large percentage of early developmental stage (EDS) cardiomyocytes (69%, n = 13; Fig. 1 c). Similarly, in the ?1 integrin /- ES cell–derived EDS cardiomyocytes, basal ICa density was depressed in half of the cells tested by 44 ± 8% (n = 10). In contrast, ?1 integrin-/- cells did not exhibit CCh-mediated inhibition of basal ICa (n = 37) (Fig. 1, b, c, and e). To rule out that a shift in the voltage dependence of ICa underlies the CCh effect after the step to 0 mV, the current–voltage (I/V) relationship was defined in the presence and absence of CCh (n = 5). As depicted in Fig. 1 f, no CCh-mediated depression of ICa was observed at the chosen potentials.  ?+ B  ^+ C8 x9 L2 t. ?8 [) \
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Figure 1. Muscarinic modulation of basal ICa is absent in ?1 integrin-/- ES cell–derived cardiomyocytes. (a) Time course of peak ICa in a representative wt EDS ES cell–derived cardiomyocyte. Prominent inhibition of basal ICa was observed after application of carbachol (1 μM). The CCh effect could be reversed by washout. Each data point in the time course was evoked by a 20-ms depolarization from a holding potential of -50 mV to a test potential of 0 mV. The upper trace indicates the holding current. (b) A representative ?1 integrin-/- EDS cardiomyocyte demonstrates the absence of the CCh-induced inhibition of basal ICa (50 ms depolarization from -50 mV to 0 mV). The time of current recordings (1 and 2, inset) is indicated. (c) Percentage of wt and ?1 integrin-/- cardiomyocytes displaying CCh-induced inhibition of basal ICa. (d) ICa density in wt cells with CCh response. (e) ICa density in ?1 integrin-/- cells in absence and presence of CCh. (f) Traces (left) and I/V relationship (right) of ICa recorded in an ES cell–derived EDS ?1 integrin-/- cardiomyocyte (1, control; 2, in presence of CCh). ICa was evoked by 50-ms depolarizations from -40 mV to  40 mV in 10-mV increments (holding potential, -50 mV). *Indicates statistical significance (paired t test, p-value , r! ]: c' E4 B& J% C
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?-adrenoceptor–mediated stimulation of ICa was preserved in ?1 integrin-/- cardiomyocytes0 q8 P5 |7 a9 B% K

The absence of muscarinic signaling could be explained by perturbed intracellular signaling components including AC, cAMP, or protein kinase A. Therefore, we determined whether additional regulatory pathways, and in particular the ?-adrenoceptor–mediated stimulation of ICa, were defective in ?1 integrin-/- cardiomyocytes. All wild-type (wt) late developmental stage (LDS) cardiomyocytes exhibited an increased current density (41.4 ± 6.8%, n = 11; Fig. 2 , a and d) after prestimulation with the ?-adrenoceptor agonist isoprenaline (ISO) (0.1 μM). Similarly, ISO increased the ICa density by 64.6 ± 12% (n = 20) in all ?1 integrin-/- cardiomyocytes tested (Fig. 2, b and e). This ISO-induced upregulation of ICa was accompanied by a slight left shift in the I/V relationship (n = 5). However, superfusion with varying concentrations (1–10 μM) of CCh after ?-adrenergic prestimulation did not suppress ICa in these cardiomyocytes (n = 20; Fig. 2, c and e). By contrast, CCh-inhibited ISO prestimulated ICa by 37.3 ± 7% (n = 11; Fig. 2 c) in 82% of wt LDS cells.
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Figure 2. Absence of muscarinic inhibition of ISO-prestimulated ICa in ?1 integrin-/- ES cell–derived cardiomyocytes. (a) ICa in a representative wt LDS ES cell–derived cardiomyocyte demonstrated prominent stimulation by ISO (0.1 μM) and subsequent inhibition by CCh. The CCh effect was reversed by ISO washout. (b) ICa in a representative ?1 integrin-/- ES cell–derived LDS cardiomyocyte displayed strong stimulation by ISO; however, no inhibition by subsequent application of CCh was observed. (c) Percentage of wt and ?1 integrin-/- cardiomyocytes displaying CCh-induced depression of ISO-prestimulated ICa. (d) ICa density in wt cells with ISO and CCh response. (e) ICa density in ?1 integrin-/- cells exposed to ISO and CCh. (f–g) Immunostaining for Gi expression in wt (f) and ?1 integrin-/- ES cell–derived cardiomyocytes (g). *Indicates statistical significance (paired t test, p-value + j- Y5 y6 _" A1 a4 g! D8 A
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To address whether the signaling defect was caused by a quantitative reduction of muscarinic receptors, QNB saturation binding studies were performed. There were no differences in the Bmax values for ? integrin-/- (33.7 ± 1.3 fmol/mg membrane protein) and wt ES cells (33.3 ± 3.1 fmol/mg membrane protein); therefore, a change in total muscarinic binding sites was excluded. Furthermore, immunocytochemical studies proved that Gi is expressed in both, wt (Fig. 2 f) and ?1 integrin-/- (Fig. 2 g) cardiomyocytes.
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Muscarinic signaling was restored in ?1 integrin rescue ES cell–derived cardiomyocytes
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To confirm that the muscarinic signaling defect was evoked specifically by the absence of the ?1 integrin gene, we next investigated the hormonal modulation of ICa in ?1 integrin rescue ES cell–derived cardiomyocytes. As depicted in Fig. 3 a, muscarinic receptor activation inhibited basal ICa by 32.3 ± 6% (Fig. 3 d) in almost half (42%, n = 19; Fig. 3 d) of the EDS cells. In 36% (n = 33; Fig. 3 c) of the LDS ?1 integrin rescue cardiomyocytes, CCh (1-10 μM) suppressed ISO prestimulated ICa by 21 ± 6% (Fig. 3, b and d). The reduction in muscarinic inhibition and altered morphology of ?1 integrin reconstituted cells (see Fig. 5 i) suggests that the rescue was incomplete.

Figure 3. Muscarinic modulation of ICa is restored in ?1 integrin rescue ES cell–derived cardiomyocytes. Other signaling cascades are intact in ?1 integrin-/- ES cell–derived cardiomyocytes. (a) Basal ICa in a representative ?1 integrin rescue EDS cardiomyocyte was inhibited by CCh. The CCh effect could be reversed by wash out. (b) ISO-prestimulated ICa in a representative ?1 integrin rescue LDS cardiomyocyte was depressed by CCh. The CCh effect was reversed by ISO washout. (c) Percentage of ?1 integrin rescue EDS and LDS cardiomyocytes responding to application of CCh or ISO and CCh. (d) Statistical analysis of ICa density in ?1 integrin rescue EDS and LDS cardiomyocytes with CCh or ISO and CCh responses. (e) The NOS inhibitor L-NMMA (0.2 mM) augmented basal ICa in ?1 integrin-/- EDS cardiomyocytes. This effect could be partially reversed by agonist wash out. (f) Atrial natriuretic peptide (ANP; 0.1 μM) induced a prominent depression of ICa in ?1 integrin-/- LDS cells. *Indicates statistically significant difference (paired t test, p-value
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Figure 5. Distribution pattern of Gi in wt, ?1 integrin-/-, ?1-integrin rescue, and cytochalasin D–treated cardiomyocytes. (a, b, and f) Deconvolution microscopy was used to characterize the distribution pattern of Gi in wt (a), ?1 integrin-/- (b), and cytochalasin D–treated (10–20 μM for 4 h) cardiomyocytes (f). (e) Distribution of Gi in ?1 integrin rescue cardiomyocytes. (c and d) Gs staining in wt (c) and ?1 integrin-/- cardiomyocytes (d). (g, h, and i) DIC images of wt (g), ?1 integrin-/- (h), and ?1 integrin rescue (i) cardiomyocytes. Bars: (a–f) 5 μm; (g–i) 25 μm.

Hence, functional responses suggest that ?1 integrin deletion resulted in a selective loss of muscarinic modulation of both, basal and ISO-prestimulated ICa, which could be restored upon reexpression of ?1 integrins.
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Nitric oxide and atrial natriuretic peptide–mediated depression of ICa is functionally intact in ?1 integrin-/- cardiomyocytes

We next asked whether other signaling pathways involved in the regulation of ICa besides the ?-adrenoceptor were affected by the ?1 integrin deficit. Previous work in our group demonstrated constitutive nitric oxide synthase (NOS) 2 activity in EDS cells (Ji et al., 1999). Consistent with this observation, the NOS inhibitor N-methyl-L-arginine (L-NMMA, 0.2 mM) enhanced ICa by 30 ± 4% in the majority (75%, n = 8) of ?1 integrin-/- EDS cells tested (Fig. 3 e). Similarly, as described for wt cardiomyocytes (Ji et al., 1999; Maltsev et al., 1999), application of the nonselective phosphodiesterase (PDE) inhibitor isobutylmethylxanthine (0.2 mM) increased the ICa density by 67.4 ± 7% (n = 7) in ?1 integrin-/- EDS, and by 41.9 ± 7% (n = 8) in ?1 integrin-/- LDS cardiomyocytes. The PDE2 subtype-specific antagonist erythro-9-(2-hydroxyl-3-nonyl)adenine (30 μM) also increased ICa density by 28.4 ± 5% (n = 7) in ?1 integrin-/- EDS cardiomyocytes. Together, these findings demonstrate that these principal signaling cascades remained intact in ?1 integrin-/- cardiomyocytes. By contrast to the missing CCh effect, atrial natriuretic peptide (ANP; rat fragment 3–28, 0.1 μM) depressed basal ICa density by 31.9 ± 3.9% (n = 8) in ?1 integrin-/- cardiomyocytes (Fig. 3 f) as previously reported for wt ES cell–derived cardiomyocytes (Ji et al., 1999).
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The defect in muscarinic signaling was found to reside at the G protein level$ L- r. |  ~) G, D8 s
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M2 receptor expression and key intracellular signaling cascades appeared intact in ?1 integrin-/- cardiomyocytes. Therefore, we next examined whether the signaling defect was related to dysfunction of the pertussis toxin–sensitive G protein Gi. We previously reported that intracellular application of GTP--S in guinea pig cardiomyocytes resulted in a slight stimulation of ICa (Hescheler et al., 1987), due to the opposing action of Gs and Gi proteins. Fig. 4 demonstrates that GTP--S induced a small increase in ICa by 35.9 ± 18% (n = 11; Fig. 4, c and d) in wt LDS cardiomyocytes. Similarly, in integrin-reconstituted ES cell–derived cardiomyocytes, GTP--S induced a small increase (46 ± 18.5%, n = 4) of ICa (data not shown). By contrast, GTP--S caused a significant augmentation of ICa density (130.4 ± 37%, n = 12; Fig. 4, b–d) in ?1 integrin-/- LDS cardiomyocytes that otherwise declined steadily in the absence of GTP--S (Fig. 4 a). This suggests that the predominant action of GTP--S is to activate Gs and that the ?1 integrin-related signaling defect is due to a disturbance in G protein signaling.
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Figure 4. Role of G proteins in the muscarinic signaling defect. (a and b) Time course of peak ICa in a representative ?1 integrin-/- LDS cardiomyocyte in the absence (a) or presence (b) of GTP--S (0.3 mM) in the patch pipette. (c) Current density of control ICa after break in and upon maximal stimulation by GTP--S in wt and ?1 integrin-/- cardiomyocytes. (d) The percentage increase in ICa was significantly greater in the ?1 integrin-/- cardiomyocytes. *Indicates statistically significant difference (unpaired t test, p-value
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Unlike adult cardiomyocytes, EDS and LDS cells did not respond to agonists of other Gi-coupled receptors including adenosine or somatostatin. Therefore, we used GTP--S–mediated activation of IK,ACh to examine Gi-coupling pathways. Although IK,ACh could be activated in wt (n = 14) and ?1 integrin rescue cells (n = 4, data not shown), no GTP--S–evoked activation of IK,ACh could be observed in ?1 integrin-/- cardiomyocytes (n = 14), consistent with the effect on ICa regulation.

Gi distribution is altered in ?1 integrin-/- ES cell–derived cardiomyocytes  _& Z6 |  z( f7 `! |
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Immunostaining was used to investigate the patterns of G protein expression. Antibodies against G subunits were used because of the large number of possible ? and  subunits that could be involved in receptor coupling. Differences in the cellular distribution of Gi and Gs, Go, the M2 receptor, and L-type Ca2  channels (VDCC) (data not shown) were found in wt cells. The vast majority of murine embryonic (68.2 ± 11.7%, n = 489) and wt ES cell–derived (64.0 ± 24.0%, n = 210) cardiomyocytes displayed a network-like pattern of Gi expression, whereas relatively few (20.8 ± 3.6%, n = 441) ?1 integrin-/- cardiomyocytes displayed this pattern. Consistent with this finding, nearly all cells in whole embryoid bodies (EBs) (90.8 ± 7.9%, n = 1,000, five experiments) exhibited a linear network-like distribution pattern of Gi expression, whereas few (10.5 ± 6.1%, n = 800, 4 experiments) ?1 integrin-/- EBs had this pattern.

We next investigated the subcellular distribution of these G proteins at higher resolution using deconvolution microscopy. Cardiomyocytes, which were identified by -actinin staining (Kolossov et al., 1998), exhibited a linear- and network-like distribution pattern of Gi in wt (Fig. 5 a) cells and diffuse staining of Gs in the wt (Fig. 5 c) cells. ?1 integrin-/- ES cell–derived cardiomyocytes exhibited diffuse staining for Gi (Fig. 5 b) and Gs (Fig. 5 d). Consistent with our functional results, a network-like distribution was also observed in the ?1 integrin rescue cells (Fig. 5 e), whereas Go was diffusely distributed throughout all cells (data not shown). In addition to distinct patterns of Gi distribution, ?1 integrin-/- ES cell–derived cardiomyocytes had different shapes as compared with the wt (Fig. 5, g and h) (Fassler et al., 1996). ?1 integrin rescue cells showed a partial morphological reversal (Fig. 5 i).
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Together, these findings are consistent with the electrophysiological results, which suggest that Gi underlies the muscarinic signaling defect.% L: q  k) P9 H
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Gi colocalizes with focal contact proteins) `; m. e" V8 H8 u. C9 ]

A similar network-like distribution of Gi has been reported for ?1 integrin focal adhesion–associated molecules such as talin and vinculin (Kostin et al., 1998; Imanaka-Yoshida et al., 1999). We have identified a unique architecture for Gi in the ?1 integrin-/- cells and found that talin and vinculin exhibit a similar diffuse distribution as Gi (data not shown). We subsequently analyzed the interaction between ?1 integrins, talin, and vinculin with Gi using deconvolution and electron microscopy. These approaches failed to demonstrate a close colocalization of ?1 integrins and Gi (Fig. 6 a). Deconvolution microscopy did reveal, however, spatial colocalization between Gi and both vinculin and talin (Fig. 6, b and c) in wt cells. Electron microscopy of double immunogold–labeled cells further revealed a relatively close colocalization between Gi and vinculin (40–150 nm) in embryonic (Fig. 6 d) and adult mouse/rat cardiomyocytes (Fig. 6 e), suggesting that direct protein–protein interaction is highly unlikely.
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Figure 6. Gi, talin, and vinculin colocalize in wt cells. (a and b) Deconvolution microscopy of ES cell–derived cells exhibited no overlap of Gi and ?1 integrins (a), but colocalization of Gi and talin (b). (c) Higher magnification showing colocalization of Gi and talin. (d and e) Immunogold staining for Gi and vinculin in murine embryonic cardiomyocytes (d) and in adult mouse heart (e). Vinculin and Gi were labeled with 5- and 15-nm gold particles, respectively. The distance between pairs of gold particles was 40–150 nm. Bars: (a and b) 6 μm; (c) 2.5 μm; (d and e) 90 nm.
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Cytochalasin D recapitulates the functional and morphological changes induced by ?1 integrin deficiency
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To further test whether muscarinic signaling is dependent on the integrity of the cytoskeleton, isolated murine embryonic cardiomyocytes (E11.5, E17.5) were incubated with cytochalasin D (10–20 μM) to immobilize the cytoskeleton. Cytochalasin D–treated cardiomyocytes (E11.5) displayed fast run down of ICa, and the majority (85.7%) of cells tested were unresponsive to CCh (n = 21). By contrast, CCh depressed basal ICa by 45.7 ± 3% (n = 5) in 60% of untreated cells. ISO-mediated stimulation of ICa remained intact in cytochalasin D pretreated murine cardiomyocytes (n = 5, E17.5). Cytochalasin D treatment also induced a diffuse distribution of Gi in both, ES cell–derived cells and murine embryonic cardiomyocytes (Fig. 5 f), consistent with the pattern observed in the ?1 integrin-/- cardiomyocytes.4 J1 G. a  k3 g& ~% z

Discussion
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In this study, we provide evidence that ?1 integrin deletion results in a selective loss of muscarinic signaling in cardiomyocytes. This signaling defect is clearly related to the lack of ?1 integrins because reconstitution with ?1 integrins reversed the altered morphology and the muscarinic signaling in ?1 integrin-/- ES cells.

Evidence for Gi dysfunction in ?1 integrin-/- cardiomyocytes

To date, ? integrins have been shown to play an important role primarily in tyrosine kinase–mediated signaling (see for review Ruoslahti, 1997). Furthermore, integrin-dependent cell anchorage regulates the efficiency of signaling from G protein–coupled receptors (Short et al., 2000). In ?1 integrin-/- cardiomyocytes, except for an absence of muscarinic regulation of ICa and IK,ACh, no other signaling defects could be detected. Our functional data pinpoint the signaling defect at the level of Gi, as intracellular dialysis with GTP--S resulted in pronounced enhancement of Gs-mediated stimulation of ICa and defective activation of IK,ACh in the ?1 integrin-/- cardiomyocytes. In previous investigations, antibody neturalization of integrins in situ revealed a role for integrins in the modulation of ICa or IBa (Wu et al., 1998). A recent study found that cat cardiomyocytes cultured on laminin-coated cover slips had disturbed cholinergic and ?-adrenergic responses (Wang et al., 2000a, b). These data are consistent with our findings and indicate a critical role for integrins in both receptor coupling and ion channel modulation. Clark et al. (1998) suggested that changes in integrin matrix interactions might underlie defects in signaling molecule clustering. Herein, we provide mechanistic evidence that receptor expression remains unchanged upon ?1 integrin deletion; however, the altered distribution pattern of Gi proteins is observed only under conditions that also result in a muscarinic signaling defect.
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Role of the cytoskeleton in G protein function& W3 n$ b7 ^6 ^( P4 y5 W+ B
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Our work also demonstrates a focal adhesion–related intracellular distribution for Gi, but not Gs, and Go. The idea that components of the cytoskeleton can determine G protein localization and function (for review see Janmey, 1998) has been proposed before by Ueda et al. (1997) and Ibarrondo et al. (1995) who found a direct association between the 12 subunit and between Gq/G11 and actin filaments, respectively. Furthermore, the activation and release of Gi2 from the cytoskeleton has been reported (Sarndahl et al., 1996). Consistent with our functional findings, the subcellular distribution of Gi was severely altered by the ?1 integrin deficiency in cardiomyocytes. Our findings are also consistent with previous studies using antisense approaches (Kleuss et al., 1991, 1993), where, in contrast to G protein knock-out models (Rudolph et al., 1996), selective G protein–mediated receptor–effector coupling could not be reconstituted at all by other G proteins. This is also supported by recent work, where selective G protein ?/ complexes were shown to be involved in the inhibition of neuronal Ca2  channels (Diverse-Pierluissi et al., 2000). This study suggests that the specificity of signal transduction is not only related to protein–protein interaction, but also to the spatial arrangement of signaling components.
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Due to the similar cellular distribution observed for Gi and that reported for the focal contact–associated proteins talin and vinculin, we tested their spatial relation to one another in cardiomyocytes. In wt cardiomyocytes, Gi and both talin and vinculin colocalized, whereas they did not in ?1 integrin-/- cardiomyocytes. The association between these molecules was further demonstrated by inhibiting the polymerization of G actin to F actin with cytochalasin D (Vasioukhin et al., 2000), which resulted in a similar alteration in cellular morphology and signaling, as observed in ?1 integrin-/- cells.3 F) r) c$ @' K) z* G

The parallel relationship between cellular structure and function that we observe in cardiomyocytes suggests that ?1 integrins play an important role in organizing the spatial association between signaling molecules such as Gi and focal adhesion–associated proteins. A similar relationship was demonstrated for caveolin (Anderson, 1998) and PDZ domains (Fanning and Anderson, 1999). It was proposed that anchoring proteins of the PSD-95/SAP90 family are required for G protein stimulation of the membrane-associated synaptic ion channel Kir3.2c (Hibino et al., 2000). An important role of signaling microdomains in cardiomyocytes has been demonstrated by Jurevicius and Fischmeister (1996), who demonstrated that colocalization of AC/cAMP and PDEs leads to a tight regulated local control of the ISO-mediated upregulation of ICa.  Y) z% k' G( X$ b

Thus, we propose that ?1 integrin deletion results in altered cytoskeletal architecture due to the loss of important anchorage proteins. This causes spatial displacement of Gi proteins and both a physical and functional disruption of signaling microdomains./ [+ H1 L0 u; B2 X( T$ M9 N
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Materials and methods

ES cell preparation' i# ~9 z' H8 D7 M
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Studies used ES cells of the D3 line (wt) (Doetschman et al., 1985; Wobus et al., 1991), the heterozygous ?1 integrin /- ES cell line, the ?1 integrin-/- ES cell line generated on D3 background (Fassler et al., 1995), and the ?1 integrin rescue ES cell line. This line was generated by reexpressing ?1 integrins in ?1 integrin-/- ES cells under control of the phosphoglycerate kinase promoter. wt and ?1 integrin /- ES cells were cultured and differentiated into spontaneously beating cardiomyocytes as previously described (Wobus et al., 1991). EDS cells were derived from EBs plated for 3–4 d and LDS cells from EBs 9–12 d after plating. ?1 integrin-/- and ?1 integrin rescue ES cells were grown on gelatin-coated cover slips without feeder cells in medium supplemented with 5 ng/ml recombinant human leukemia inhibitory factor (Fassler et al., 1996). ?1 integrin-/- ES cells were cultured for 3 d in hanging drops and 3 d in suspension before plating on gelatin-coated plates. Because of the delayed onset of beating, ?1 integrin-/- cardiomyocytes derived from EBs plated for 6–10 d were defined as EDS, and those cultured for 14–18 d were defined as LDS cells (Fassler et al., 1996). ?1 integrin rescue ES cells were maintained in presence of G418 (300 μg/ml). Single cardiomyocytes were isolated from clusters of spontaneously beating areas as previously described (Ji et al., 1999). Murine embryonic (E11.5, E17.5) ventricular cardiomyocytes were harvested from superovulated mice (Fleischmann et al., 1998). The heart was removed from the embryo, and the cardiomyocytes were isolated by collagenase digestion.4 h; \2 ?8 R8 V' ~+ U# r' W3 l. ]( E
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Cytochalasin D treatment1 X  }6 K4 E  |3 g3 r( U& q7 p
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12 h after dissociation, murine embryonic cardiomyocytes were treated with cytochalasin D for times indicated (15 min–16.5 h). Murine embryonic cells were incubated with 10 μM cytochalasin D and ES cell–derived cardiomyocytes with 20 μM cytochalasin D.
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Electrophysiology
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Only spontaneously beating single cardiomyocytes were selected for patch clamp recordings as previously described (Ji et al., 1999; Viatchenko-Karpinski et al., 1999). Whole cell or perforated patch measurements yielded similar results. Depolarizing pulses lasting 20 or 50 ms were applied at a frequency of 0.33 and 0.2 Hz, respectively, with an Axopatch 200-A (Axon Instruments, Inc.) or an EPC-9 amplifier (Heka). For most recordings, currents were leak subtracted using a P/n leak subtraction protocol (trace in Fig. 3, not leak subtracted). Data were acquired at a sampling rate of 2–10 kHz, filtered at 1 kHz, stored on hard disk, and analyzed off-line using the ISO2 (MFK) or Pulse/Pulse-Fit (Heka) software package. Analysis of the (CCh) and the ISO effects was performed as previously described. Only those cells exhibiting a change in ICa density of >5% after ISO or CCh application were analyzed. The GTP--S effect was compared with the response of untreated cells on the same experimental day. The average change in ICa density was calculated during the period of 180 s after break in (wt, n = 9; ?1 integrin-/-, n = 10; rescue cardiomyocytes, n = 5) and subtracted from the GTP--S response.: N9 H0 f5 B! m) U
2 R, P- }/ v, D% f; w$ ?3 F
IK,ACh recordings were made with equimolar K  concentrations to increase current amplitudes and GTP--S (0.3 mM) in the pipette. Voltage ramp depolarizations (150 ms, -100–80 mV) were applied at a frequency of 0.1 Hz immediately after break in. Control ramp currents obtained before current activation were subtracted from IK,ACh currents, which were evident 1–2 min after membrane rupture. All currents were recorded from cells adhered to glass cover slips in a temperature-controlled (35°C) recording chamber. For ICa, the internal solution contained 120 mM CsCl, 3 mM MgCl2, 5 mM MgATP, 10 mM EGTA, 5 mM Hepes, pH 7.4 (adjusted with CsOH), the external solution contained 120 mM NaCl, 5 mM KCl, 3.6 mM CaCl2, 20 mM TEA-Cl, 1 mM MgCl2, 10 mM Hepes, pH 7.4 (adjusted with TEA-OH). For IK,ACh, the internal solution contained 40 mM KCl, 100 mM kaspartate, 5 mM MgATP, 2 mM EGTA, and 10 mM Hepes (pH adjusted to 7.4 with KOH), and the extracellular solution contained 140 mM KCl, 2 mM MgCl2, 5 mM NaCl, 1.8 mM CaCl2, 5 mM glucose, and 5 mM Hepes (pH adjusted to 7.4 with KOH). For perforated patch clamp recordings, amphotericin B (Sigma-Aldrich) was dissolved in DMSO and used at a final concentration of 250–500 μg/ml. Current measurements were initiated after the series resistance reached 10–20 M. All substances were obtained from Sigma-Aldrich.; @  T/ C5 ~& D/ D$ x8 I# d
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Data are expressed as mean ± SEM, paired or unpaired Student's t test was used to analyze electrophysiological data, and a p-value of ) X# |' l* J8 v) p  I) |

Radioligand binding assays  M% s2 U! G1 h  x9 s' t3 ]0 ~
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EBs were harvested, and membrane homogenates were prepared. In brief, homogenates were incubated for 3 h at 22°C in 25 mM sodium phosphate (pH 7.4) containing 5 mM MgCl2. Saturation binding experiments were carried out with (-)-N-quinuclidinyl benzilate (-QNB; 42.0 Ci/mmol; NEN Life Science Products). Binding was performed with saturating concentrations of each radioligand (2 nM), and nonspecific binding was defined as binding in the presence of 2.5 μM atropine. Protein concentrations were determined by the Bradford method.) C: o- f8 K6 g7 p7 f  d( W) A. h

Immunocytochemistry$ M8 F, k  j3 t: E6 E3 k6 ]

Whole EBs, EB and murine embryonic heart-derived isolated cells were incubated overnight with antibodies at 4°C. Antibodies included mouse anti–rat actinin (1:800; Sigma-Aldrich), monoclonal antitalin or antivinculin (1:100; Chemicon International), polyclonal rat anti–mouse ?1 integrin (Sigma-Aldrich), rabbit polyclonal anti-M2 receptor (1:2,000; R&D Systems), 1C (1:200; Alomone Labs), and anti–G protein -subunits. G protein antibodies against Gi1–3, Go, and Gs subunits (Santa Cruz Biotechnology, Inc.) were used at a 1:500 dilution. Next, cells were stained with Cy3- (or Cy2-) conjugated goat anti–rabbit (or goat anti–mouse) Ig (1:800, Sigma-Aldrich) and with a biotinylated goat anti–mouse (or anti–rabbit) IgG (1:400, Dako) or a biotinylated anti–rat antibody (Amersham Pharmacia Biotech) followed by streptavidin-conjugated Alexa FluorTM488 (1:200; Mo Bi Tec), streptavidin-conjugated Cy2 (1:100, Amersham Pharmacia Biotech), or Cy3 (1:600, Amersham Pharmacia Biotech).% |. I6 j& \$ G, w$ G/ u, W
( C- t; n- t, v
Morphological analysis

The distribution pattern of Gi was analyzed by fluorescence microscopy (ZEISS) in wt (EDS, 7 3; LDS, 7 11), ?1 integrin-/- (EDS, 6 10; LDS, 6 14), and murine embryonic cardiomyocytes (E11.5/12.5, E17.5). All cardiomyocytes, identified by -actinin costaining were analyzed on each slide (ranging from 49 to 174). Statistical analysis was performed using the unpaired Student's t test. DIC pictures were taken using an appropriately equipped Axiovert 135 microscope (ZEISS).5 i+ u9 k* Q) Z1 S- r/ w

Deconvolution microscopy' ^. I3 s2 P7 K: r: ^0 s

For each specimen, an image stack (64 planes) was collected using an inverted ZEISS Axiovert 100 M with a ZEISS 63 Planapochromat oil immersion objective. Bandpass filter sets for Cy2 and Cy3 (AHF) without overlap in the fluorescence emission spectrum were used. The thickness of each optical section was 100 nm to assure appropriate optical resolution for the subsequent iterative deconvolution process. A three-dimensional deconvolution algorithm was applied to the image stacks (Schaefer, 1997) to increase the image resolution for colocalization (KS 400; ZEISS). Both theoretical point-spread functions (PSFs) based on microscope and specimen data and measured PSFs, calculated from fluorescent beads with a diameter of 1 μm (Molecular Probes, T-14792), were used. A conjugated gradient maximum likelihood deconvolution algorithm with 60 iterations was performed on the image stacks. Output intensities were scaled between gray value 0 and 255. Generally, theoretical and measured PSFs generated comparable results with slightly higher resolution in the resulting images by using the measured PSFs.
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Immunogold labeling
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Adult and embryonic mouse or rat heart tissue was perfused with 4% PFA in 0.1 M PBS or with 2% PFA/2% glutardialdehyde at 4°C. After fixation, the tissue was embedded with araldit or LR-white. The tissue grids were treated with 50 mM TBS for 15 min and blocked with 4% BSA in TBS for 1 h at room temperature. Then, primary antibodies against vinculin or talin (1:30–50) or ?1 integrin (1:100; PharMingen) and a rabbit polyclonal antibody for Gi were used at room temperature for 2 h. Grids were rinsed four times with 0.05% Tween-20–TBS, incubated with 5-nm gold-conjugated anti–mouse (or anti–rabbit) IgG (1:30–50) for 1 h, and treated with biotinylated goat anti–rabbit or anti–mouse (1:50; Dako) for 1 h at room temperature. Finally, specimens were incubated with 15-nm gold-labeled streptavidin (1:30; Zymed Laboratories) for 1 h followed by 2% glutardialdehyde fixation for 10 min. For contrast, grids were treated with 2% uranylacetate in 70% ethanol for 20 min and PBS for 7 min.

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