有没有朋友研究BFGF这种物质？它去分化的机理是什么？

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Basic FGF, also known as FGF-2, has a wide range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration.Basic FGF has been used as a essential component of embryonic stem cell culture medium to maintain cells in an undifferentiated state. " [2 i/ S# k5 M

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( ~7 |( p( p$ s& E% q对不起，我没有做个这个实验，不敢乱说。还有就是关于信号通路还有小分子的作用等，我觉得现在的结论很是不完善，现在paper的观点都是实验真正做出来了，才提出通路，而不是靠逻辑推理来看是否起激活或者是抑制作用，您还不如直接使一下，看到底对您的细胞起什么作用，这样的结果更加放心。7 I8 r; N  K0 X/ `0 t- u7 E% M% c* ?
从前人发表文章的观点来看，bFGF对维持外胚层EpiSC状态起到很重要的作用，想要更进一步达到内细胞团ES状态，则需要激活Lif/BMP4/2i等小分子蛋白控制的通路或者是依靠Klf2,klf4.Nanog,Nr5a2过表达等等