凝聚胺？什么作用？

yuanyan2010

A highly efficient method of gene trasfer into mammalian cells is through infection with retroviral vectors. The efficiency of retroviral infection is enhanced significantly, 100 to 1,000 fold in some cells, by including polybrene during the infection. Polybrene with DMSO shock is also used to mediate DNA transfer into a variety of cell types, such as: CHO, chicken embryo fibroblases, NIH3T3 cells, and myeloid cells.
9 n* B7 O4 m: b6 ]* oView All »

yuanyan2010

Polybrene Infection / Transfection Reagent
, f0 N3 J4 y& w/ ~, Y. B

yuanyan2010

回复 huangcong1988 的帖子
# {  I% i8 T9 r% \+ }' S; A
7 E3 n, ~9 J  x常规都加的，终浓度在4-8ug/ml

yuanyan2010

millipore

yuanyan2010

回复 huangcong1988 的帖子
8 f4 Z# U7 W( J+ e1 T- X; r/ }0 J& _) \  z$ Q
你到millipore官网看看吧。我具体也不知道多少钱

yuanyan2010

回复 huangcong1988 的帖子* H: l& W# j0 i: q2 G

) M; s3 ^7 y5 |* w3 j是这个，用的时候直接加到培养液就可以了，使终浓度是4-8ug/ml