Cold Spring Harb Perspect Biol

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http://cshperspectives.cshlp.org ... 12401.full.pdf+html9 Z& N" c: t) \. I
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Deconstructing Pancreas Developmental Biology9 I5 W# |" b( @- ^
Cecil M. Benitez1,4, William R. Goodyer1,4 and Seung K. Kim1,2,3, C* l3 I1 k' R2 d  U2 P% q
1Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305-5329
# f* G9 Z' i( k& z2Department of Medicine (Oncology Division), Stanford University School of Medicine, Stanford, California 94305-5329% U# d  b6 d) y/ v. j% B
3Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305-5329  t! h0 }9 K( N2 P% Y5 c
Correspondence: seungkim{at}stanford.edu
% F  I& g5 p2 A; H" n0 u' eAbstract
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" z; e# k4 L. E6 V0 V/ @" L% kThe relentless nature and increasing prevalence of human pancreatic diseases, in particular, diabetes mellitus and adenocarcinoma, has motivated further understanding of pancreas organogenesis. The pancreas is a multifunctional organ whose epithelial cells govern a diversity of physiologically vital endocrine and exocrine functions. The mechanisms governing the birth, differentiation, morphogenesis, growth, maturation, and maintenance of the endocrine and exocrine components in the pancreas have been discovered recently with increasing tempo. This includes recent studies unveiling mechanisms permitting unexpected flexibility in the developmental potential of immature and mature pancreatic cell subsets, including the ability to interconvert fates. In this article, we describe how classical cell biology, genetic analysis, lineage tracing, and embryological investigations are being complemented by powerful modern methods including epigenetic analysis, time-lapse imaging, and flow cytometry-based cell purification to dissect fundamental processes of pancreas development.

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