Nat Protocol

flydayzhu

http://www.nature.com/nprot/jour ... nprot.2011.444.html
2 x% H) t$ t: l3 {; L. {5 q4 N; G4 F. }( i5 t- W# ^3 ~6 y' H& p
Nat Protoc. 2012 Jan 19;7(2):256-67. doi: 10.1038/nprot.2011.444.- }! j$ b- Q4 B
Using formaldehyde-assisted isolation of regulatory elements (FAIRE) to isolate active regulatory DNA.8 t' E5 J: f6 h' P% a+ r9 R! A
Simon JM, Giresi PG, Davis IJ, Lieb JD.3 L9 o$ C$ p- s1 L: U- ]: }
Source
6 M, r5 I. k4 K' F; z4 g: \2 a  FLineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
. N' Q' b; s2 z) y3 {5 r" R# w% Z; B1 \( w8 B, j% S
Abstract
  `6 A' ~5 @# b" i, J' pEviction or destabilization of nucleosomes from chromatin is a hallmark of functional regulatory elements in eukaryotic genomes. Historically identified by nuclease hypersensitivity, these regulatory elements are typically bound by transcription factors or other regulatory proteins. FAIRE (formaldehyde-assisted isolation of regulatory elements) is an alternative approach to identify these genomic regions and has proven successful in a multitude of eukaryotic cell and tissue types. Cells or dissociated tissues are cross-linked briefly with formaldehyde, lysed and sonicated. Sheared chromatin is subjected to phenol/chloroform extraction and the isolated DNA, typically encompassing 1-3% of the human genome, is purified. We provide guidelines for quantitative analysis by PCR, microarrays or next-generation sequencing. Regulatory elements enriched by FAIRE have high concordance with those identified by nuclease hypersensitivity or chromatin immunoprecipitation (ChIP), and the entire procedure can be completed in 3 d. FAIRE has low technical variability, which allows its usage in large-scale studies of chromatin from normal or diseased tissues.

hzh0525

11111111111111111111111111              

boya888

11 11 11